A　new　magneto-controlled　microfluidic　device　for　direct　electrochemical　determination　of　squamous　cell　carcinoma　antigen　(SCC-Ag)　in　serum　was　designed　by　using　anti-SCC　antibody　(SCC-Ab)-functionalized　magnetic　mesoporous　nanogold/thionine/NiCo2O4　hybrid　nanostructures　as　immunosensing　probes　(P-1-Ab)　and　horseradish　peroxidase-SCC-Ab　conjugates-labeled　nanogold/graphene　nanosheets　as　signal　tags　(P-2-Ab).　In　the　presence　of　the　analyte　SCC-Ag,　the　sandwich　immunocomplex　was　formed　between　the　immunosensing　probes　and　the　signal　tags.　With　the　aid　of　an　external　magnet,　the　formed　immunocomplex　was　attached　to　the　microfluidic　device.　The　assay　was　implemented　in　newborn　calf　serum　(NBCS)　containing　2.5　mM　H2O2　based　on　the　labeled　peroxidase　on　the　P-2-Ab　toward　the　catalytic　reduction　of　H2O2.　Under　optimal　conditions,　the　increase　in　the　current　was　proportional　to　the　concentration　of　SCC-Ag　from　2.5　pg/mL　to　15　ng/mL.　The　detection　limit　(LOD)　was　1.0　pg/mL　SCC-Ag　at　3s(B).　The　electrochemical　immunoassay　displayed　an　acceptable　precision,　selectivity　and　stability.　Clinical　serum　specimens　were　assayed　with　the　method,　and　the　results　were　in　acceptable　agreement　with　those　obtained　from　the　referenced　electrochemiluminescent　method.　Importantly,　the　method　can　be　suitable　for　on-line　use　in　the　mass　production　of　miniaturized　lab-on-a-chip　devices　and　open　a　new　opportunity　for　protein　diagnostics　and　biosecurity.　(C)　2011　Elsevier　B.V.　All　rights　reserved.