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[期刊论文]

Magneto-controlled electrochemical immunosensor for direct detection of squamous cell carcinoma antigen by using serum as supporting electrolyte

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author:

Li, Q. (Li, Q..) [1] | Tang, D. (Tang, D..) [2] | Tang, J. (Tang, J..) [3] | Unfold

Indexed by:

Scopus

Abstract:

A new magneto-controlled microfluidic device for direct electrochemical determination of squamous cell carcinoma antigen (SCC-Ag) in serum was designed by using anti-SCC antibody (SCC-Ab)-functionalized magnetic mesoporous nanogold/thionine/NiCo 2O 4 hybrid nanostructures as immunosensing probes (P 1-Ab) and horseradish peroxidase-SCC-Ab conjugates-labeled nanogold/graphene nanosheets as signal tags (P 2-Ab). In the presence of the analyte SCC-Ag, the sandwich immunocomplex was formed between the immunosensing probes and the signal tags. With the aid of an external magnet, the formed immunocomplex was attached to the microfluidic device. The assay was implemented in newborn calf serum (NBCS) containing 2.5mM H 2O 2 based on the labeled peroxidase on the P 2-Ab toward the catalytic reduction of H 2O 2. Under optimal conditions, the increase in the current was proportional to the concentration of SCC-Ag from 2.5pg/mL to 15ng/mL. The detection limit (LOD) was 1.0pg/mL SCC-Ag at 3s B. The electrochemical immunoassay displayed an acceptable precision, selectivity and stability. Clinical serum specimens were assayed with the method, and the results were in acceptable agreement with those obtained from the referenced electrochemiluminescent method. Importantly, the method can be suitable for on-line use in the mass production of miniaturized lab-on-a-chip devices and open a new opportunity for protein diagnostics and biosecurity. © 2011 Elsevier B.V.

Keyword:

Functional graphene nanosheets; Magneto-controlled electrochemical immunosensor; Microfluidic device; Multifunctional magnetic beads; Squamous cell carcinoma antigen

Community:

  • [ 1 ] [Li, Q.]Key Lab. of Analysis and Detection for Food Safety, Ministry of Education and Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
  • [ 2 ] [Li, Q.]College of Pharmaceutical Sciences, Zunyi Medical College, Zunyi 563003, China
  • [ 3 ] [Tang, D.]Key Lab. of Analysis and Detection for Food Safety, Ministry of Education and Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
  • [ 4 ] [Tang, J.]Key Lab. of Analysis and Detection for Food Safety, Ministry of Education and Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
  • [ 5 ] [Su, B.]Key Lab. of Analysis and Detection for Food Safety, Ministry of Education and Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
  • [ 6 ] [Chen, G.]Key Lab. of Analysis and Detection for Food Safety, Ministry of Education and Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, China
  • [ 7 ] [Wei, M.]Institute of New Energy Technology and Nano-Materials, Fuzhou University, Fuzhou 350002, China

Reprint 's Address:

  • [Tang, D.]Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education and Fujian Province, Department of Chemistry, Fuzhou University, Fuzhou 350108, China

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Source :

Biosensors and Bioelectronics

ISSN: 0956-5663

Year: 2011

Issue: 1

Volume: 27

Page: 153-159

5 . 6 0 2

JCR@2011

1 0 . 7 0 0

JCR@2023

JCR Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 52

30 Days PV: 1

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