Query：
学者姓名：陈国南
Refining：
Year
Type
Indexed by
Source
Complex
Former Name
Co-
Language
Clean All
Abstract ：
目的:建立高效液相色谱质谱联用(HPLC-MS)测定青风藤中生物碱的方法。方法:以70%乙醇进行索氏提取,HPLC-MS分析方法定性,HPLC分析方法定量。色谱条件为反相色谱柱Agilent ZORBAX SB-C_(18)(5μm,3.0mm×250mm),柱温:30℃,进样量:10μL,流动相甲醇(A)-5mmol/L醋酸铵缓冲液(B)=55∶45(V∶V),流速:0.5mL/min,进行线性梯度洗脱。结果:在最佳的分离条件下,5种生物碱在25min内得到很好的分离效果,且适用于青风藤中青藤碱和木兰花碱的定量分析。经测定,所购青风藤药材中青藤碱和木兰花碱的含量分别为6.11和3.22mg/...
Keyword ：
木兰花碱 木兰花碱 生物碱 生物碱 青藤碱 青藤碱 青风藤 青风藤 高效液相色谱质谱联用技术 高效液相色谱质谱联用技术
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 王丽丽 , 王静楠 , 许惠凤 et al. 高效液相色谱质谱联用技术同时测定青风藤生物碱的方法 [J]. | 中华中医药杂志 , 2021 , 36 (09) : 5606-5609 . |
| MLA | 王丽丽 et al. "高效液相色谱质谱联用技术同时测定青风藤生物碱的方法" . | 中华中医药杂志 36 . 09 (2021) : 5606-5609 . |
| APA | 王丽丽 , 王静楠 , 许惠凤 , 余丽双 , 叶蕻芝 , 陈国南 et al. 高效液相色谱质谱联用技术同时测定青风藤生物碱的方法 . | 中华中医药杂志 , 2021 , 36 (09) , 5606-5609 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
以鲫鱼为代表性研究对象,采用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)技术,分别研究了3种农药(敌百虫、高效氯氰菊酯和吡虫啉)对其体内代谢的影响.通过Masslynx软件在MSE模式下采集数据,使用UNIFI软件进行自动检测和数据过滤,并将差异性物质与在线代谢组数据库进行比较,鉴定出甘油三酯类(TGs)物质具有明显差异.其中,吡虫啉对TGs代谢的影响最大,且肝脏的代谢差异比大脑更显著,低浓度的急性暴露诱导肝脏中的TGs积累,其含量与暴露浓度之间的关系符合Michaelis-Menten方程的增长趋势.结果表明,TGs可在短时间内(≤2 h)灵敏地反映农药吡虫啉的低浓度(≤2...
Keyword ：
农药污染 农药污染 甘油三酯 甘油三酯 生物标记物 生物标记物 超高效液相色谱-四极杆飞行时间质谱 超高效液相色谱-四极杆飞行时间质谱
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 何聿 , 夏倩 , 王文莉 et al. UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物 [J]. | 高等学校化学学报 , 2020 , 41 (03) : 431-438 . |
| MLA | 何聿 et al. "UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物" . | 高等学校化学学报 41 . 03 (2020) : 431-438 . |
| APA | 何聿 , 夏倩 , 王文莉 , 罗芳 , 陈金凤 , 郭雅婷 et al. UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物 . | 高等学校化学学报 , 2020 , 41 (03) , 431-438 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Pesticides exert a long-term negative influence on the aquatic organism and even the aquatic environment. It is necessary to find some potential biomarkers to give insight into the pesticides pollution and provide a rapid , sensitive , early-warning method. In this study , ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS) was applied to analyze the effect of pesticides on the metabolomics in liver and brain of Crucian carp. Three pesticides, namely trichlorfon, beta-cypermethrin and imidacloprid were investigated , respectively. Data was collected by masslynx software in MSE mode , analyzed by UNIFI software for automatic detection and data filtering, compared to the online metabolome database. The metabolic difference of liver was more significant than that of the brain. The triglycerides (TGs) species were identified as the different metabolites. Among these three pesticides, imidacloprid had the most significant influence on the metabolism of TGs. The low concentration of acute exposure induced the accumulation of TGs in the liver, and the relationship between the content and the exposure concentration was in line with the growth trend of Michaelis-Menten equation. The results showed that TGs can sensitively reflect the low concentration exposure of imidacloprid pesticides (<= 20 ng/mL) in a short period of time (<= 2 h) , which can be used as a potential biomarker to investigate the toxicity of imidacloprid to aquatic organisms , and help to establish a new method for estimation the pesticides contamination in aquatic environment.
Keyword ：
Biomarker Biomarker Pesticides pollution Pesticides pollution Triglycerides Triglycerides Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS) Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS)
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | He Yu , Xia Qian , Wang Wenli et al. Triglycerides as Potential Biomarkers for Pesticides Pollution in Aquatic Environment Using UPLC-QTOF-MS [J]. | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE , 2020 , 41 (3) : 431-438 . |
| MLA | He Yu et al. "Triglycerides as Potential Biomarkers for Pesticides Pollution in Aquatic Environment Using UPLC-QTOF-MS" . | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 41 . 3 (2020) : 431-438 . |
| APA | He Yu , Xia Qian , Wang Wenli , Luo Fang , Chen Jinfeng , Guo Yating et al. Triglycerides as Potential Biomarkers for Pesticides Pollution in Aquatic Environment Using UPLC-QTOF-MS . | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE , 2020 , 41 (3) , 431-438 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A novel electrochemiluminescence (ECL) biosensor was developed for high sensitive and selective detection of miRNA-21 based on the efficient and specific toehold-mediated strand displacement (TMSD) amplification with Ru(phen)(3)(2+) loaded DNA nanoclews (NCs-Ru(phen)(3)(2+)) as signal tags. The stable DNA nanoclews, synthesized by a simple rolling circle amplification reaction, were employed to load with Ru(phen)(3)(2+) efficiently as ECL signal tags to amplify the signals. As for TMSD, the substrate strand (Sub) was initially hybridized with P1 and P2 to form DNA duplex structures with a toehold 1. miRNA-21 could hybridize with the toehold 1 and trigger the TMSD amplification with the help of assist strand, releasing lots of P1 stands from DNA duplex structures. The TMSD technique realized the converting and amplification of the single miRNA-21 input to lots of output DNA (namely P1) with good selectivity, simultaneously. Output P1 were designed to expand the stem-locked region of HP, which were immobilized on the Au electrodes firstly. Subsequently, the opened HP could hybridize with the Ru(phen)(3)(2+), capturing the ECL signal tags closed to the sensing surface. The ECL intensity of the system had a linear relationship with the logarithm of the miRNA-21 concentration in the range of 1.0 fM to 100 pM with a limit of detection of 0.65 fM. The strategy was further applied to detect miRNA-21 in complex samples, and the result was consistent with the qRT-PCR.
Keyword ：
DNA nanoclews DNA nanoclews Electrochemiluminescence biosensor Electrochemiluminescence biosensor miRNA-21 miRNA-21 Ru(phen)(3)(2+) Ru(phen)(3)(2+) Toehold-mediated strand displacement Toehold-mediated strand displacement
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Ying , Xu, Guoyan , Lian, Guili et al. Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)(3)(2+) loaded DNA nanoclews as signal tags [J]. | BIOSENSORS & BIOELECTRONICS , 2020 , 147 . |
| MLA | Zhang, Ying et al. "Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)(3)(2+) loaded DNA nanoclews as signal tags" . | BIOSENSORS & BIOELECTRONICS 147 (2020) . |
| APA | Zhang, Ying , Xu, Guoyan , Lian, Guili , Luo, Fang , Xie, Qunfang , Lin, Zhenyu et al. Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)(3)(2+) loaded DNA nanoclews as signal tags . | BIOSENSORS & BIOELECTRONICS , 2020 , 147 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity was prepared. In the presence of hydrogen peroxide, the system catalyses the oxidation of terephthalic acid to a blue-fluorescent product (excitation=315nm; emission=425nm). Pyrophosphate has a very strong affinity for Cu2 ionand blocks the POx-mimicking activity of theCuBDC NS. If, however, inorganic pyrophosphatase is present, the POx mimicking activity is gradually restored because pyrophosphate is hydrolyzed. The findings were used to design a method for the determination of the activity of inorganic pyrophosphataseby fluorometry. Fluorescence increases linearly in the 1-50mU center dot ml-1inorganic pyrophosphatase activity range. The limit of detection is 0.6mU center dot m-1 (S/N=3).
Keyword ：
CuBDC nanosheet CuBDC nanosheet Hydrogen peroxide Hydrogen peroxide Inhibitors Inhibitors Peroxidase mimicking activity Peroxidase mimicking activity Two-dimensional metal-organic frameworks Two-dimensional metal-organic frameworks
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Hu, Shuisheng , Zhu, Lin , Lam, Cheong Wing et al. Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework [J]. | MICROCHIMICA ACTA , 2019 , 186 (3) . |
| MLA | Hu, Shuisheng et al. "Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework" . | MICROCHIMICA ACTA 186 . 3 (2019) . |
| APA | Hu, Shuisheng , Zhu, Lin , Lam, Cheong Wing , Guo, Longhua , Lin, Zhenyu , Qiu, Bin et al. Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework . | MICROCHIMICA ACTA , 2019 , 186 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A surface-enhanced Raman scattering (SERS) substrate with good flexibility and high water absorbing capacity is reported. It consists of a calcium alginate sponge incorporating gold nanoparticles. These are in close contactwith thesponge without the need for amino or sulfhydryl modification. The substrate is capable of detecting the dyes crystal violet (CV) and malachite green (MG) in water directly and rapidly by immersing it into the liquid sample. Preconcentration and separation are not required. The dyes absorbed on the sponge can be detected without drying and thus the whole analytical process can be completed within 3min. The results show that the lowest detectable concentrations are 0.1 and 0.25gL(-1) for CV and MG, respectively. This is lower than the minimum required performance limits set by the European Commission and the US EPA. Moreover, MG and CV can be simultaneously detected in liquid samples due to their different SERS bands (at 1216 and 1534cm(-1), respectively). It should be noted that the molecular structures of MG and CV are very similar. Therefore, the method has a large potential for determination of several analytes simultaneously even in complex sample metrics.
Keyword ：
Crystal violet Crystal violet Gold nanorods Gold nanorods Good flexibility Good flexibility High water absorbing capacity High water absorbing capacity Liquid samples Liquid samples Malachite green Malachite green Metallic nanoparticles Metallic nanoparticles On-site detection On-site detection Rapid analysis Rapid analysis Surface-enhance Raman scattering Surface-enhance Raman scattering
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Fu, Huiping , Chen, Jiaming , Chen, Lijuan et al. A calcium alginate sponge with embedded gold nanoparticles as a flexible SERS substrate for direct analysis of pollutant dyes [J]. | MICROCHIMICA ACTA , 2019 , 186 (2) . |
| MLA | Fu, Huiping et al. "A calcium alginate sponge with embedded gold nanoparticles as a flexible SERS substrate for direct analysis of pollutant dyes" . | MICROCHIMICA ACTA 186 . 2 (2019) . |
| APA | Fu, Huiping , Chen, Jiaming , Chen, Lijuan , Zhu, Xi , Chen, Zhuling , Qiu, Bin et al. A calcium alginate sponge with embedded gold nanoparticles as a flexible SERS substrate for direct analysis of pollutant dyes . | MICROCHIMICA ACTA , 2019 , 186 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Poly(procaterol hydrochloride) (p-ProH) polymeric film was successfully deposited onto the carboxyl multi-walled carbon nanotube (CMWCNT) modified glass carbon electrode (GCE) to construct a p-ProH/CMWCNT composite modified GCE. Due to the synergistic effect of p-ProH and CMWCNT in the composite, the developed sensor can enormously enhance the oxidation peak current of bromhexine hydrochloride (BrH) at ca. + 0.90 V. Based on this appearance, an electrochemical method was established for the sensitive and selective determination of BrH with differential pulse voltammetry (DPV). Various conditions affecting the peak current response of BrH were studied and optimized. Under the best conditions, the oxidation peak current of BrH is linear to its concentration in two linear dynamic ranges of 0.2-1.0 mol L-1 (R = 0.9948) and 1.0-8.0 mol L-1 (R = 0.9956), with a detection limit of 0.1 mol L-1 (S/N = 3). Interference experiment indicated that the as-prepared electrochemical sensor showed wonderful selectivity to the recognition of BrH and was free from disturbance of many other electro-active substances such as dopamine, ascorbic and uric acid. Finally, the practicability of the BrH sensor was verified by the satisfactory results acquired from the BrH determination in pharmaceutical preparation and human serum.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Kong, Dexian , Han, Libin , Wang, Zeming et al. An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride [J]. | RSC ADVANCES , 2019 , 9 (21) : 11901-11911 . |
| MLA | Kong, Dexian et al. "An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride" . | RSC ADVANCES 9 . 21 (2019) : 11901-11911 . |
| APA | Kong, Dexian , Han, Libin , Wang, Zeming , Jiang, Lili , Zhang, Qian , Wu, Qiong et al. An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride . | RSC ADVANCES , 2019 , 9 (21) , 11901-11911 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
电致化学发光(ECL)检测技术因其具有无需激发光源、仪器简单、灵敏度高、选择性好等特点,被广泛应用于环境分析、生物分析等领域.温度是影响ECL的主要因素之一,在传统的ECL传感器中大多是通过溶液整体加热的方法来控制温度,这种方法操作繁琐,且溶液中的热不稳定性物质及易挥发性物质容易受到影响,因此电极很少工作在最适宜的温度下.热控电极技术可以只提高电极表面温度,而维持溶液的整体温度不变,使用起来具有很好的便利性.作者课题组首次将热控电极引入到ECL传感器的构建中,由于电极表面和溶液之间存在一定的温度梯度,因此可以引发强制对流,从而加快物质的扩散和对流速率;电极表面温度的升高还可以进一步提高电极表面物质的电化学反应速率,这两方面的共同作用提高了ECL检测的灵敏度.同时,利用热控电极可以解决整体加热所引起的背景信号升高,挥发性、热不稳定性物质易受温度影响等问题,而且通过电极加热的方法可去除电极表面的污染物,从而提高ECL检测的重现性.本文综述了近年来基于热控电极技术的ECL传感器的研究进展,主要介绍了热控电极的加热方式、电极种类以及热控电极在ECL中的应用等,并分析了该技术在实际应用中面临的主要问题,对该技术未来的发展趋势进行了展望.
Keyword ：
传感器 传感器 热控电极 热控电极 电致化学发光 电致化学发光
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 张惠芳 , 陈毅挺 , 罗芳 et al. 基于热控电极的电致化学发光传感器的研究进展 [J]. | 电化学 , 2019 , 25 (2) : 172-184 . |
| MLA | 张惠芳 et al. "基于热控电极的电致化学发光传感器的研究进展" . | 电化学 25 . 2 (2019) : 172-184 . |
| APA | 张惠芳 , 陈毅挺 , 罗芳 , 林振宇 , 陈国南 . 基于热控电极的电致化学发光传感器的研究进展 . | 电化学 , 2019 , 25 (2) , 172-184 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
采用紫外可见吸收光谱法和荧光光谱法研究N6-异戊烯基腺嘌呤与牛血清白蛋白间的相互作用.通过Stern-Volmer方程法和紫外可见吸收光谱法,确定N6-异戊烯基腺嘌呤对牛血清白蛋白的荧光猝灭作用主要为静态猝灭;推断两者之间的结合常数在温度为290、295、302、310 K时分别为162143、13400、4326和74353,结合位点数分别为1.1814、0.9074、0.8102和1.1121,结合力主要为氢键或范德华力;通过 Δλ 为60、15 nm时荧光同步扫描光谱的位移情况,推断出N6-异戊烯基腺嘌呤与牛血清白蛋白的结合使牛血清白蛋白的构象发生了一定变化.
Keyword ：
N6-异戊烯基腺嘌呤 N6-异戊烯基腺嘌呤 牛血清白蛋白 牛血清白蛋白 荧光光谱法 荧光光谱法
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 叶桦珍 , 陈国南 . N6-异戊烯基腺嘌呤与牛血清白蛋白的相互作用研究 [J]. | 福州大学学报（自然科学版） , 2019 , 47 (4) : 550-553,559 . |
| MLA | 叶桦珍 et al. "N6-异戊烯基腺嘌呤与牛血清白蛋白的相互作用研究" . | 福州大学学报（自然科学版） 47 . 4 (2019) : 550-553,559 . |
| APA | 叶桦珍 , 陈国南 . N6-异戊烯基腺嘌呤与牛血清白蛋白的相互作用研究 . | 福州大学学报（自然科学版） , 2019 , 47 (4) , 550-553,559 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
建立了一种利用改性纳米金粒子富集与毛细管电泳-电化学发光(CE-ECL)法测定水产品中4种氟喹诺酮(环丙沙星、恩诺沙星、氧氟沙星、诺氟沙星)类药物残留的分析方法.实验考察了富集条件与CE分离条件,并基于增强Ru (bpy)32+电化学发光的原理,优化了ECL检测条件.结果 表明,在最优条件下,经改性金纳米粒子富集后的4种分析物在0.05 ～ 10.0 μmol/L浓度范围内,其峰高与浓度呈现良好的线性关系,检出限(S/N=3)可达0.2μmol/L,4种目标物的富集倍数达104～ 127倍.该方法用于鳗鱼样品的分析,回收率为94.5％ ～ 112％,相对标准偏差(RSD)均不大干6.3％.
Keyword ：
富集 富集 改性纳米金粒子 改性纳米金粒子 毛细管电泳-电化学发光(CE-ECL) 毛细管电泳-电化学发光(CE-ECL) 氟喹诺酮药物 氟喹诺酮药物
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈宗保 , 王星 , 尹月春 et al. 改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留 [J]. | 分析测试学报 , 2019 , 38 (2) : 176-181 . |
| MLA | 陈宗保 et al. "改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留" . | 分析测试学报 38 . 2 (2019) : 176-181 . |
| APA | 陈宗保 , 王星 , 尹月春 , 陈国南 . 改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留 . | 分析测试学报 , 2019 , 38 (2) , 176-181 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
