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学者姓名：陈国南
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Abstract ：
以鲫鱼为代表性研究对象,采用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)技术,分别研究了3种农药(敌百虫、高效氯氰菊酯和吡虫啉)对其体内代谢的影响.通过Masslynx软件在MSE模式下采集数据,使用UNIFI软件进行自动检测和数据过滤,并将差异性物质与在线代谢组数据库进行比较,鉴定出甘油三酯类(TGs)物质具有明显差异.其中,吡虫啉对TGs代谢的影响最大,且肝脏的代谢差异比大脑更显著,低浓度的急性暴露诱导肝脏中的TGs积累,其含量与暴露浓度之间的关系符合Michaelis-Menten方程的增长趋势.结果表明,TGs可在短时间内(≤2 h)灵敏地反映农药吡虫啉的低浓度(≤2...
Keyword ：
农药污染 农药污染 甘油三酯 甘油三酯 生物标记物 生物标记物 超高效液相色谱-四极杆飞行时间质谱 超高效液相色谱-四极杆飞行时间质谱
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| GB/T 7714 | 何聿 , 夏倩 , 王文莉 et al. UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物 [J]. | 高等学校化学学报 , 2020 , 41 (03) : 431-438 . |
| MLA | 何聿 et al. "UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物" . | 高等学校化学学报 41 . 03 (2020) : 431-438 . |
| APA | 何聿 , 夏倩 , 王文莉 , 罗芳 , 陈金凤 , 郭雅婷 et al. UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物 . | 高等学校化学学报 , 2020 , 41 (03) , 431-438 . |
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A novel electrochemiluminescence (ECL) biosensor was developed for high sensitive and selective detection of miRNA-21 based on the efficient and specific toehold-mediated strand displacement (TMSD) amplification with Ru(phen)(3)(2+) loaded DNA nanoclews (NCs-Ru(phen)(3)(2+)) as signal tags. The stable DNA nanoclews, synthesized by a simple rolling circle amplification reaction, were employed to load with Ru(phen)(3)(2+) efficiently as ECL signal tags to amplify the signals. As for TMSD, the substrate strand (Sub) was initially hybridized with P1 and P2 to form DNA duplex structures with a toehold 1. miRNA-21 could hybridize with the toehold 1 and trigger the TMSD amplification with the help of assist strand, releasing lots of P1 stands from DNA duplex structures. The TMSD technique realized the converting and amplification of the single miRNA-21 input to lots of output DNA (namely P1) with good selectivity, simultaneously. Output P1 were designed to expand the stem-locked region of HP, which were immobilized on the Au electrodes firstly. Subsequently, the opened HP could hybridize with the Ru(phen)(3)(2+), capturing the ECL signal tags closed to the sensing surface. The ECL intensity of the system had a linear relationship with the logarithm of the miRNA-21 concentration in the range of 1.0 fM to 100 pM with a limit of detection of 0.65 fM. The strategy was further applied to detect miRNA-21 in complex samples, and the result was consistent with the qRT-PCR.
Keyword ：
DNA nanoclews DNA nanoclews Electrochemiluminescence biosensor Electrochemiluminescence biosensor miRNA-21 miRNA-21 Ru(phen)(3)(2+) Ru(phen)(3)(2+) Toehold-mediated strand displacement Toehold-mediated strand displacement
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| GB/T 7714 | Zhang, Ying , Xu, Guoyan , Lian, Guili et al. Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)(3)(2+) loaded DNA nanoclews as signal tags [J]. | BIOSENSORS & BIOELECTRONICS , 2020 , 147 . |
| MLA | Zhang, Ying et al. "Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)(3)(2+) loaded DNA nanoclews as signal tags" . | BIOSENSORS & BIOELECTRONICS 147 (2020) . |
| APA | Zhang, Ying , Xu, Guoyan , Lian, Guili , Luo, Fang , Xie, Qunfang , Lin, Zhenyu et al. Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru(phen)(3)(2+) loaded DNA nanoclews as signal tags . | BIOSENSORS & BIOELECTRONICS , 2020 , 147 . |
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采用紫外可见吸收光谱法和荧光光谱法研究N6-异戊烯基腺嘌呤与牛血清白蛋白间的相互作用.通过Stern-Volmer方程法和紫外可见吸收光谱法,确定N6-异戊烯基腺嘌呤对牛血清白蛋白的荧光猝灭作用主要为静态猝灭;推断两者之间的结合常数在温度为290、295、302、310 K时分别为162143、13400、4326和74353,结合位点数分别为1.1814、0.9074、0.8102和1.1121,结合力主要为氢键或范德华力;通过 Δλ 为60、15 nm时荧光同步扫描光谱的位移情况,推断出N6-异戊烯基腺嘌呤与牛血清白蛋白的结合使牛血清白蛋白的构象发生了一定变化.
Keyword ：
N6-异戊烯基腺嘌呤 N6-异戊烯基腺嘌呤 牛血清白蛋白 牛血清白蛋白 荧光光谱法 荧光光谱法
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| GB/T 7714 | 叶桦珍 , 陈国南 . N6-异戊烯基腺嘌呤与牛血清白蛋白的相互作用研究 [J]. | 福州大学学报（自然科学版） , 2019 , 47 (4) : 550-553,559 . |
| MLA | 叶桦珍 et al. "N6-异戊烯基腺嘌呤与牛血清白蛋白的相互作用研究" . | 福州大学学报（自然科学版） 47 . 4 (2019) : 550-553,559 . |
| APA | 叶桦珍 , 陈国南 . N6-异戊烯基腺嘌呤与牛血清白蛋白的相互作用研究 . | 福州大学学报（自然科学版） , 2019 , 47 (4) , 550-553,559 . |
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建立了一种利用改性纳米金粒子富集与毛细管电泳-电化学发光(CE-ECL)法测定水产品中4种氟喹诺酮(环丙沙星、恩诺沙星、氧氟沙星、诺氟沙星)类药物残留的分析方法.实验考察了富集条件与CE分离条件,并基于增强Ru (bpy)32+电化学发光的原理,优化了ECL检测条件.结果 表明,在最优条件下,经改性金纳米粒子富集后的4种分析物在0.05 ～ 10.0 μmol/L浓度范围内,其峰高与浓度呈现良好的线性关系,检出限(S/N=3)可达0.2μmol/L,4种目标物的富集倍数达104～ 127倍.该方法用于鳗鱼样品的分析,回收率为94.5％ ～ 112％,相对标准偏差(RSD)均不大干6.3％.
Keyword ：
富集 富集 改性纳米金粒子 改性纳米金粒子 毛细管电泳-电化学发光(CE-ECL) 毛细管电泳-电化学发光(CE-ECL) 氟喹诺酮药物 氟喹诺酮药物
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| GB/T 7714 | 陈宗保 , 王星 , 尹月春 et al. 改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留 [J]. | 分析测试学报 , 2019 , 38 (2) : 176-181 . |
| MLA | 陈宗保 et al. "改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留" . | 分析测试学报 38 . 2 (2019) : 176-181 . |
| APA | 陈宗保 , 王星 , 尹月春 , 陈国南 . 改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留 . | 分析测试学报 , 2019 , 38 (2) , 176-181 . |
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电致化学发光(ECL)检测技术因其具有无需激发光源、仪器简单、灵敏度高、选择性好等特点,被广泛应用于环境分析、生物分析等领域.温度是影响ECL的主要因素之一,在传统的ECL传感器中大多是通过溶液整体加热的方法来控制温度,这种方法操作繁琐,且溶液中的热不稳定性物质及易挥发性物质容易受到影响,因此电极很少工作在最适宜的温度下.热控电极技术可以只提高电极表面温度,而维持溶液的整体温度不变,使用起来具有很好的便利性.作者课题组首次将热控电极引入到ECL传感器的构建中,由于电极表面和溶液之间存在一定的温度梯度,因此可以引发强制对流,从而加快物质的扩散和对流速率;电极表面温度的升高还可以进一步提高电极表面物质的电化学反应速率,这两方面的共同作用提高了ECL检测的灵敏度.同时,利用热控电极可以解决整体加热所引起的背景信号升高,挥发性、热不稳定性物质易受温度影响等问题,而且通过电极加热的方法可去除电极表面的污染物,从而提高ECL检测的重现性.本文综述了近年来基于热控电极技术的ECL传感器的研究进展,主要介绍了热控电极的加热方式、电极种类以及热控电极在ECL中的应用等,并分析了该技术在实际应用中面临的主要问题,对该技术未来的发展趋势进行了展望.
Keyword ：
传感器 传感器 热控电极 热控电极 电致化学发光 电致化学发光
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| GB/T 7714 | 张惠芳 , 陈毅挺 , 罗芳 et al. 基于热控电极的电致化学发光传感器的研究进展 [J]. | 电化学 , 2019 , 25 (2) : 172-184 . |
| MLA | 张惠芳 et al. "基于热控电极的电致化学发光传感器的研究进展" . | 电化学 25 . 2 (2019) : 172-184 . |
| APA | 张惠芳 , 陈毅挺 , 罗芳 , 林振宇 , 陈国南 . 基于热控电极的电致化学发光传感器的研究进展 . | 电化学 , 2019 , 25 (2) , 172-184 . |
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Lead ions (Pb2+) cause harm to human health. Therefore, the development of fast, effective, and convenient sensors for Pb2+ monitoring has received great attention. In this study, a portable method has been proposed for Pb2+ detection using normal electronic balance as a readout. Magnetic bead-catalytic strand is hybridized with platinum nanoparticles (Pt NPs) functioned substrate strand (Pt-Sub) to form double-stranded DNA first. In the presence of Pb2+, the DNAzyme is activated and cleaved at the ribo-adenosine site of the substrate strand and hence causes Pt NPs to be released into the supernatant, which can be easily separated from the Pt-Sub by a magnet. The separated Pt NPs can effectively catalyze the decomposition of H2O2 to produce O-2. In a sealed bottle, the pressure inside the bottle is increased by the generation of oxygen so that the water is discharged from the drainage device, and the weight of the water can be easily and precisely measured by a normal electronic balance. The weighting water has a linear relationship with the concentration of Pb2+ in the range of 2.5-100 nM and the detection limit of 0.83 nM (S/N = 3). The proposed method has been applied to detect Pb2+ in water with satisfactory results. Because the electronic balance is one of the most commonly used analytical tools for the laboratory, it is very practical and convenient without the need for expensive instruments and complicated data processing.
Keyword ：
electronic balance electronic balance lead ions lead ions magnetic beads magnetic beads platinum nanoparticles platinum nanoparticles portable sensor portable sensor
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| GB/T 7714 | Huang, Yaying , Lin, Cuiying , Luo, Fang et al. Ultrasensitive and Portable Assay for Lead(II) Ions by Electronic Balance as a Readout [J]. | ACS SENSORS , 2019 , 4 (9) : 2465-2470 . |
| MLA | Huang, Yaying et al. "Ultrasensitive and Portable Assay for Lead(II) Ions by Electronic Balance as a Readout" . | ACS SENSORS 4 . 9 (2019) : 2465-2470 . |
| APA | Huang, Yaying , Lin, Cuiying , Luo, Fang , Qiu, Bin , Guo, Longhua , Lin, Zhenyu et al. Ultrasensitive and Portable Assay for Lead(II) Ions by Electronic Balance as a Readout . | ACS SENSORS , 2019 , 4 (9) , 2465-2470 . |
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Hydrogen sulfide (H2S) is a highly toxic gas, which poses a serious threat to human health and safety. It is necessary to develop some simple and highly selective and sensitive approach for the detection of hydrogen sulfide. In this study, a highly selective and sensitive fluorescence sensor for H2S detection has been developed on the basis of Cu(II)-dependent DNAzyme. The catalytic DNA strand (Cu-enzyme) was labeled with a quencher (black hole quencher, BHQ1) at the 5'-termini and its corresponding DNA substrate strand (Cu-substrate) was labeled with a 6-carboxyfluorescein (FAM fluorophore) at the 3'-end and an additional quencher was attached on the 5'-end. Cu-enzyme and Cu-substrate could hybridize with each other to form a triplex structure firstly and the FAM emission was quenched by the labeled quenchers. The present of Cu(II) could catalyze the oxidative cleavage of the Cu-substrate and hence caused the releasing of the quencher from the FAM fluorophore and the enhanced fluorescence of the system. However, in the presence of H2S, Cu(II) reacted with H2S to form CuS firstly, hindering the cleavage and the fluorescence enhancement The decrease of fluorescence intensity had a linear relationship with the concentration of H2S in the range between 0.5 and 25 mu M with a detection limit of 0.2 mu M (S/N = 3). The proposed method innovatively used fluorescent labeling technology and DNAzyme sensing to detect gaseous hydrogen sulfide, and it has been successfully applied to detect the contamination of hydrogen sulfide gas in the refuse collectors with a simple purification procedure.
Keyword ：
Cu(II)-dependent DNAzyme Cu(II)-dependent DNAzyme Fluorescence sensor Fluorescence sensor Hydrogen sulfide Hydrogen sulfide
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| GB/T 7714 | Yue, Guiyin , Huang, Da , Luo, Fang et al. Highly selective fluorescence sensor for hydrogen sulfide based on the Cu (II)-dependent DNAzyme [J]. | JOURNAL OF LUMINESCENCE , 2019 , 207 : 369-373 . |
| MLA | Yue, Guiyin et al. "Highly selective fluorescence sensor for hydrogen sulfide based on the Cu (II)-dependent DNAzyme" . | JOURNAL OF LUMINESCENCE 207 (2019) : 369-373 . |
| APA | Yue, Guiyin , Huang, Da , Luo, Fang , Guo, Longhua , Qiu, Bin , Lin, Zhenyu et al. Highly selective fluorescence sensor for hydrogen sulfide based on the Cu (II)-dependent DNAzyme . | JOURNAL OF LUMINESCENCE , 2019 , 207 , 369-373 . |
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A copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity was prepared. In the presence of hydrogen peroxide, the system catalyses the oxidation of terephthalic acid to a blue-fluorescent product (excitation=315nm; emission=425nm). Pyrophosphate has a very strong affinity for Cu2 ionand blocks the POx-mimicking activity of theCuBDC NS. If, however, inorganic pyrophosphatase is present, the POx mimicking activity is gradually restored because pyrophosphate is hydrolyzed. The findings were used to design a method for the determination of the activity of inorganic pyrophosphataseby fluorometry. Fluorescence increases linearly in the 1-50mU center dot ml-1inorganic pyrophosphatase activity range. The limit of detection is 0.6mU center dot m-1 (S/N=3).
Keyword ：
CuBDC nanosheet CuBDC nanosheet Hydrogen peroxide Hydrogen peroxide Inhibitors Inhibitors Peroxidase mimicking activity Peroxidase mimicking activity Two-dimensional metal-organic frameworks Two-dimensional metal-organic frameworks
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| GB/T 7714 | Hu, Shuisheng , Zhu, Lin , Lam, Cheong Wing et al. Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework [J]. | MICROCHIMICA ACTA , 2019 , 186 (3) . |
| MLA | Hu, Shuisheng et al. "Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework" . | MICROCHIMICA ACTA 186 . 3 (2019) . |
| APA | Hu, Shuisheng , Zhu, Lin , Lam, Cheong Wing , Guo, Longhua , Lin, Zhenyu , Qiu, Bin et al. Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework . | MICROCHIMICA ACTA , 2019 , 186 (3) . |
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脑神经相关的各种生理和病理过程需在众多生理活性物质的共同参与下完成.硫化氢(H2 S)作为第三类内源性气体信号分子,在维持中枢神经系统正常的生理机能方面起重要的调节作用.因此,在活体层次实现H2 S的精准定量分析,将极大推动人类揭秘脑神经过程中的分子机制的研究进程.然而,H2 S具有较强的还原性和易挥发性,其物理化学性质与脑内含巯基化合物相似,实现H2 S的高选择性的分离分析成为其检测的关键科学问题.本文综述了近年来各类型H2 S检测方法的设计原理及研究进展,对相关研究的发展前景进行了展望.
Keyword ：
活体检测方法 活体检测方法 硫化氢 硫化氢 评述 评述
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| GB/T 7714 | 陈仲辉 , 陈宇 , 翁爱彬 et al. 气体信号分子硫化氢的活体检测方法研究进展 [J]. | 分析化学 , 2019 , 47 (10) : 1572-1579 . |
| MLA | 陈仲辉 et al. "气体信号分子硫化氢的活体检测方法研究进展" . | 分析化学 47 . 10 (2019) : 1572-1579 . |
| APA | 陈仲辉 , 陈宇 , 翁爱彬 , 罗芳 , 郭隆华 , 邱彬 et al. 气体信号分子硫化氢的活体检测方法研究进展 . | 分析化学 , 2019 , 47 (10) , 1572-1579 . |
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Foodborne contaminants widely exist in foods, which can lead to various foodborne diseases and food safety issues. The development of quick, sensitive and universal analytical approaches for foodborne contaminants is imperative. Electrochemiluminescent functional nucleic acids (ECL FNAs)-based sensors are a series of sensing devices using FNAs as the recognition elements and ECL as the transducer. Contributing to the specific recognition ability of FNA and the high sensitivity of ECL, ECL FNA-based sensors are considered to be of great application potential for foodborne contaminants monitoring. This review mainly presents the applications of ECL FNA-based sensors for foodborne contaminants (including microorganisms, mycotoxins, allergens, antibiotics, heavy metal ions, pesticides and some illegal additives). In general, the application of ECL FNA-based sensors in the field of food analysis is just in its infancy. Although there are several limitations and challenges, it is envisaged that ECL FNA-based sensors will have broad prospects for food analysis in the future.
Keyword ：
electrochemiluminescent (ECL) electrochemiluminescent (ECL) foodborne contaminants foodborne contaminants functional nucleic acids (FNAs) functional nucleic acids (FNAs) sensor sensor
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| GB/T 7714 | Xu, Huifeng , Zhu, Xi , Wang, Jian et al. Electrochemiluminescent functional nucleic acids-based sensors for food analysis [J]. | LUMINESCENCE , 2019 , 34 (3) : 308-315 . |
| MLA | Xu, Huifeng et al. "Electrochemiluminescent functional nucleic acids-based sensors for food analysis" . | LUMINESCENCE 34 . 3 (2019) : 308-315 . |
| APA | Xu, Huifeng , Zhu, Xi , Wang, Jian , Lin, Zhenyu , Chen, Guonan . Electrochemiluminescent functional nucleic acids-based sensors for food analysis . | LUMINESCENCE , 2019 , 34 (3) , 308-315 . |
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