Common　colorimetric　immunoassays　only　show　monochromatic　intensity　variations,　and　the　absorbance　intensity　is　utilized　for　the　quantitative　detection　of　the　analytes.　Herein,　we　demonstrate　for　the　first　time　that　Au　Nanobipyramid@Ag　Nanorods　(Au　NBP@Ag)　is　an　ideal　multicolor　chromogenic　substrate　for　immunoassays　which　combines　the　absorbance　intensity　with　the　Localized　Surface　Plasmon　Resonance　(LSPR)　peak　shifts　are　utilized　for　quantitative　detection　of　the　analytes,　and　vivid　color　variations　are　used　for　semi-quantitative　detection.　Our　investigation　reveals　that　unlike　the　single　metallic　nanoparticles　such　as　gold　nanorods　which　show　one-way　LSPR　peak　shifts,　Au　NBP@Ag　Nanorods　shows　an　interesting　multi-way　peak　shifts:　the　LSPR　peak　would　initially　blue　shift,　and　then　red　shift,　finally　blue　shift　again　during　the　etching　process.　As　a　result,　a　series　of　vivid　colors　can　be　observed.　The　results　indicate　that　the　correlation　linear　range　for　the　detection　of　Squamous　cell　carcinoma　antigen　(SCCA)　was　2.5-105　ng/mL.　Although　only　the　detection　of　SCCA　is　demonstrated　in　this　work,　this　method　is　easy　to　expand　to　detect　other　biomarkers　since　our　method　is　based　on　conventional　immunoassay　strategy.　(C)　2018　Elsevier　B.V.　All　rights　reserved.