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学者姓名：唐点平
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Abstract ：
The yolk-shell structure has become a research hotspot in the field of photocatalysis in recent years due to its stability, movable yolk, and internal voids. However, yolk-shell has not been fully explored and applied in photoelectrochemical (PEC) immunoassays. In this work, a smartphone-based portable PEC immunoassay was constructed by using high-entropy yolk-shell Au@(ZnCdMnGaCu)(x)S as a photosensitive material, showing satisfactory detection with the assistance of localized surface plasmon resonance (LSPR). Specifically, a typical sandwich reaction introduces a detection antibody modified and labeled with gold nanoparticles (Au NPs) of alkaline phosphatase (ALP) into the system with the presence of prostate-specific antigen (PSA). Ascorbic acid (AA) content increased with increasing PSA and showed a linear enhancement of photocurrent at PSA concentrations of 0.01-50 ng mL(-1) with a limit of detection of 8.94 pg mL(-1). Additionally, the proposed assay technology not only provides a portable detection system for the immediate detection of tumor markers, but also offers a promising paradigm for the development of high-entropy materials.
Keyword ：
High-entropy yolk-shell Au@(ZnCdMnGaCu)(x)S High-entropy yolk-shell Au@(ZnCdMnGaCu)(x)S Localized surface plasmon resonance Localized surface plasmon resonance Photoelectrochemical immunoassay Photoelectrochemical immunoassay Portable inspection system Portable inspection system Prostate-specific antigen Prostate-specific antigen Smartphone Smartphone
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| GB/T 7714 | Wan, Xinyu , Wang, Xin , Wang, Haiyang et al. Localized surface plasmon resonance-enhanced photoelectrochemical immunoassay based on high-entropy yolk-shell Au@(ZnCdMnGaCu)xS [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 422 . |
| MLA | Wan, Xinyu et al. "Localized surface plasmon resonance-enhanced photoelectrochemical immunoassay based on high-entropy yolk-shell Au@(ZnCdMnGaCu)xS" . | SENSORS AND ACTUATORS B-CHEMICAL 422 (2025) . |
| APA | Wan, Xinyu , Wang, Xin , Wang, Haiyang , Wei, Qiaohua , Tang, Dianping . Localized surface plasmon resonance-enhanced photoelectrochemical immunoassay based on high-entropy yolk-shell Au@(ZnCdMnGaCu)xS . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 422 . |
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Photocurrent-polarity conversion strategies are typically realized by constructing complex photovoltaic electrodes or changing the relevant conditions, but most involve poor photogenerated carrier transfer efficiency and cumbersome experimental steps. To this end, a photoelectrochemical (PEC) biosensor by utilizing ascorbic acid (AA)-induced photocurrent-polarity-switching was proposed for the detection of carcinoembryonic antigen (CEA). Under light excitation, the electron donor AA was oxidized by the photogenerated holes of photoactive material Co-NC/CdS, resulting in the conversion of cathodic photocurrent to the anodic direction. In the presence of the target CEA, alkaline phosphatase (ALP) was introduced into the microplates by the sandwiched immunoreaction, which then catalyzed the production of AA from ascorbic acid-2-phosphate (AAP). Finally, the catalytic product AA was transferred onto Co-NC/CdS-modified screen-printed carbon electrode, thus activating photocurrent-polarity-switching platform. The anodic photocurrent values gradually increased with increasing CEA concentration in the range of 0.02-80 ng mL(-1) and reached a limit of detection (LOD) of 8.47 pg mL(-1) (S/N = 3). In addition, the results of actual sample detection prove the reliability of the constructed PEC biosensor. Importantly, this work relies on a mobile smartphone wireless Bluetooth device coupled with the PEC biosensor for immediate detection, providing another idea for detecting CEA in clinical diagnosis.
Keyword ：
Carcinoembryonic antigen Carcinoembryonic antigen Photocurrent-polarity-switching Photocurrent-polarity-switching Photoelectrochemical immunoassay Photoelectrochemical immunoassay Point-of-care Point-of-care Wireless bluetooth Wireless bluetooth
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| GB/T 7714 | Wang, Xin , Wang, Haiyang , Wan, Xinyu et al. Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 267 . |
| MLA | Wang, Xin et al. "Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching" . | BIOSENSORS & BIOELECTRONICS 267 (2025) . |
| APA | Wang, Xin , Wang, Haiyang , Wan, Xinyu , Wei, Qiaohua , Zeng, Yongyi , Tang, Dianping . Smartphone-based point-of-care photoelectrochemical immunoassay coupling with ascorbic acid-triggered photocurrent-polarity conversion switching . | BIOSENSORS & BIOELECTRONICS , 2025 , 267 . |
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The judicious utilization of antibiotics has established a robust bulwark for human health. However, their improper usage has engendered deleterious ramifications on the environment, underscoring the imperative for developing efficacious and cost-effective detection and degradation platforms. This study presents a sulfur-modified iron-cobalt bimetallic single-atom nitrogen-doped carbon catalyst (S-FeCo-NC) with a noncopper active center. In contrast to conventional laccase, which utilizes copper as its active center, the S-FeCo-NC catalyst exhibits multiple enzyme activities, including laccase-like, peroxidase-like, and catalase-like functions, with iron and cobalt serving as the active centers. As a proof of concept, the combined laccase-like and catalase-like functions of S-FeCo-NC were used as independent signal outputs, while a multienzyme cascade dual-mode assay system was designed for the rapid detection of tetracycline (TC) in combination with peroxidase-like enzymes. In this system, oxygen directly participated in the catalytic process of laccase-like as an electron acceptor, while catalase-like peroxidase efficiently catalyzed the production of O2 from H2O2. The elevated concentration of O2 offered a unique advantage for the increased catalytic activity of the laccase-like enzyme, which outputs visually resolved colorimetric signals using stable 4-aminopyridine with oxidized TC. Furthermore, the peroxidase-like activity of S-FeCo-NC catalyzed the generation of OH radicals with strong oxidative properties, and these radicals carried out effective oxidative decomposition of TC. The signal output of the response of the catalytic process was performed using differential pulse cyclic voltammetry, which further improved the sensitivity and accuracy of the detection. The experimental findings demonstrate that the detection system exhibits a favorable response signal to TC within the range of 0.005-500 mu M, with its detection range reaching 0.5-500 and 0.005-1.00 mu M, respectively, and the detection limit is as low as 0.22 mu M and 1.68 nM, respectively. This cascade dual-mode detection system, based on multienzyme activity, has been shown to significantly enhance the catalytic activity of laccase, while also demonstrating stability in a lower detection range. This suggests that it may offer a novel approach for the sensitive detection and degradation of environmental pollutants.
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| GB/T 7714 | Wang, Yunsen , Tian, Shuo , Chen, Shuyun et al. S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7526-7535 . |
| MLA | Wang, Yunsen et al. "S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7526-7535 . |
| APA | Wang, Yunsen , Tian, Shuo , Chen, Shuyun , Li, Meijin , Tang, Dianping . S-Modified MOF Nanozyme Cascade System with Multi-Enzyme Activity for Dual-Mode Antibiotic Assay . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7526-7535 . |
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Accurate and rapid identification of expired and spoiled food is crucial for food conservation, reducing resource wastage, and preventing food poisoning. This paper presents a portable electrochemical sensing platform supported by a miniature electrochemical workstation and an intelligent pH recognition system, enabling detection of L-lactic acid (L-LA) in food products without enzyme involvement. The system was based on a Cu2O@CuO multifaceted extended spatial hexapod structure. The synthesized Cu2O@CuO was characterized by a welldefined multifaceted structure. Significant enzyme-free catalysis was exhibited, and pH responses dominated by anthocyanins were identified through an intelligent image acquisition system. Additionally, we developed an electrochemical detection device for pH assistance during target testing, addressing the limitations of current electrochemical sensors' complex signal acquisition components using 3D-printed fabrication techniques and smartphones. The proposed multifunctional electrochemical workbench based on Cu2O@CuO was found to offer a preferable linear detection range of 0.1-1000 mu M for L-LA, with a low detection limit of 0.027 mu M. The visualization of pH determination was introduced as a novel approach for developing advanced electrochemical workbenches. In conclusion, pH-assisted portable electrochemical detection systems hold great potential for immediate food safety identification, particularly in resource-limited areas, facilitating prompt diagnosis and ensuring food safety.
Keyword ：
3D-printed device 3D-printed device Electrochemical detection Electrochemical detection Enzyme-free catalysis Enzyme-free catalysis Food quality evaluation Food quality evaluation Visualization Visualization
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| GB/T 7714 | Wu, Di , Wei, Qiaohua , Yu, Zhichao et al. Miniature dual-channel electrochemical 3D-printed sensing platform for enzyme-free screening of L-lactic acid in foodstuffs accompanying pH recognition [J]. | FOOD CHEMISTRY , 2025 , 465 . |
| MLA | Wu, Di et al. "Miniature dual-channel electrochemical 3D-printed sensing platform for enzyme-free screening of L-lactic acid in foodstuffs accompanying pH recognition" . | FOOD CHEMISTRY 465 (2025) . |
| APA | Wu, Di , Wei, Qiaohua , Yu, Zhichao , Gao, Yuan , Knopp, Dietmar , Tang, Dianping . Miniature dual-channel electrochemical 3D-printed sensing platform for enzyme-free screening of L-lactic acid in foodstuffs accompanying pH recognition . | FOOD CHEMISTRY , 2025 , 465 . |
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Nanozyme-based electrochemical biosensors have emerged as an alternative to enzyme-based biosensors for next-generation bioanalysis. However, potential antibody modifications limit the catalytic sites of the nanozyme, thereby reducing sensor sensitivity. Here, a sensitive method for determining carcinoembryonic antigen (CEA) was developed. It involved coupling a cascade enzyme-enzyme-like catalytic reaction using Fe-Co Prussian blue analog nanozymes with high peroxidase-like activity (79.42 U mg-1). Briefly, the transduction of biological signals to chemical signals was achieved through the strategy centered on catalytic electroactive probes. Thereafter, with the assistance of the microelectrochemical workstation, the output of signals was realized. The platform exhibited an ultra-wide range of 0.020-100 ng mL-1 and a detection limit of 0.013 ng mL-1 CEA, which was mainly attributed to the excellent peroxidase activity, good conductivity, and synergistic amplification of current signals of synthesized nanozymes. In addition, the modification-free features greatly reduced the complexity of the bioassay and significantly improves its portability and cost-effectiveness. Overall, this study advances the development of nanozymes and their electrochemical biosensing applications and is expected to extend to the development of miniaturized devices in direct detection environments.
Keyword ：
Bifunctional nanozyme Bifunctional nanozyme Carcinoembryonic antigen Carcinoembryonic antigen Catalytic amplification Catalytic amplification Electrochemical immunoassay Electrochemical immunoassay
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| GB/T 7714 | Chen, Shuyun , Tian, Shuo , Wang, Yunsen et al. Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 278 . |
| MLA | Chen, Shuyun et al. "Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay" . | BIOSENSORS & BIOELECTRONICS 278 (2025) . |
| APA | Chen, Shuyun , Tian, Shuo , Wang, Yunsen , Li, Meijin , Tang, Dianping . Harnessing bifunctional nanozyme with potent catalytic and signal amplification for innovating electrochemical immunoassay . | BIOSENSORS & BIOELECTRONICS , 2025 , 278 . |
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Sluggish charge transfer and rapid electron-hole recombination severely limit the analytical performance of photoelectrochemical (PEC) immunoassays. This work presented a PEC immunosensing strategy that employed a target-induced enzyme-catalyzed reaction to in-situ generate oxygen vacancy (Ov) for amplifying the photocurrent detection of carcinoembryonic antigen (CEA). Concretely, ascorbic acid-2-phosphate (AAP) was catalyzed to produce ascorbic acid (AA) by alkaline phosphatase (ALP) in the presence of CEA. The generated AA could serve as a reducing agent to introduce oxygen vacancy (Ov) into the etching tungsten trioxide (E-WO3) photoanode, resulting in an Ov-enriched E-WO3 (E-WO3-Ov) photoanode. The formation of Ov allowed efficient introduction of defect levels into the energy band structure of E-WO3-Ov photoanode, resulting in high charge transfer and electron-hole separation efficiency for photocurrent amplification. Later, it was applied to fabricate a PEC immunosensor, thus enabling a wide linear range from 0.02 to 80 ng/mL and a low detection limit of 12.9 pg/mL. Overall, this work presented a promising sensing strategy for PEC immunosensors, expanding the scope of potential applications in bioassays and clinical diagnostics.
Keyword ：
Charge transfer Charge transfer Defect level Defect level Electron-hole separation Electron-hole separation Oxygen vacancy Oxygen vacancy Photoelectrochemical immunoassay Photoelectrochemical immunoassay
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| GB/T 7714 | Qin, Jiao , Yu, Zhichao , Wu, Di et al. Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay [J]. | BIOSENSORS & BIOELECTRONICS , 2025 , 279 . |
| MLA | Qin, Jiao et al. "Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay" . | BIOSENSORS & BIOELECTRONICS 279 (2025) . |
| APA | Qin, Jiao , Yu, Zhichao , Wu, Di , Li, Meijin , Tang, Dianping . Target-induced oxygen vacancy on the etching WO3 photoanode for in-situ amplified photoelectrochemical immunoassay . | BIOSENSORS & BIOELECTRONICS , 2025 , 279 . |
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Colorimetric immunoassays are widely used for biomarker detection, offering advantages of simplicity, sensitivity, and cost-effectiveness. Recent advancements focus on improving the catalytic activity of nanozymes for enhancing the sensitivity and accuracy of such assays. Bimetallic CuCo Prussian blue analog (CuCo PBA) has emerged as promising candidates due to their excellent peroxidase-like activity. However, their instant synthesis and integration into immunoassays for the rapid detection of biomarkers like carcinoembryonic antigen (CEA) remain underexplored. This study presents an innovative approach using CuCo PBA nanozymes for colorimetric immunoassays with immediate generation and application. In this study, CuCo PBA nanozymes were synthesized instantly by reacting Cu2+ with K-3[Co(CN)(6)] (<1 min), and their peroxidase-like activity was exploited for a colorimetric immunoassay system targeting CEA. The system demonstrated a clear blue color change upon the reaction of CuCo PBA with H2O2 and 3,3 ',5,5 '-tetramethylbenzidine (TMB), enabling sensitive detection. The assay was optimized for various parameters, including pH, temperature, and material ratio. A linear response was obtained for CEA detection over a concentration range of 0.05-60 ng/mL with a limit of detection (LOD) of 22 pg/mL. The integration of glucose oxidase (GOx) mediated the generation of H2O2, triggering the colorimetric reaction. This instantaneous CuCo PBA-based system effectively detected CEA in human serum samples, highlighting its potential for rapid diagnostic applications. This work introduces a novel approach for rapid and sensitive colorimetric immunoassays using CuCo PBA nanozymes that are synthesized on-demand and immediately applied. The system allows for efficient CEA detection with an exceptionally low detection limit, offering great potential for clinical diagnostics. The instant generation and application of CuCo PBA nanozymes in immunoassays represent a significant advancement in point-of-care testing technologies.
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| GB/T 7714 | Li, Xiaoqin , Lin, Huizi , Chen, Xuwei et al. Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen [J]. | ANALYTICA CHIMICA ACTA , 2025 , 1354 . |
| MLA | Li, Xiaoqin et al. "Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen" . | ANALYTICA CHIMICA ACTA 1354 (2025) . |
| APA | Li, Xiaoqin , Lin, Huizi , Chen, Xuwei , Luo, Fenqiang , Zhang, Rong , Deng, Xiaoyan et al. Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen . | ANALYTICA CHIMICA ACTA , 2025 , 1354 . |
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The rise of antibiotic-resistant bacteria poses a serious global health threat, highlighting the urgent need for novel strategies beyond conventional antibiotic therapies. This study explores the potential of microbe-imprinted polymers (MIPs) as innovative, pathogen-specific affinity agents. Utilizing microbial surface-initiated polymerization, MIPs are in-situ synthesized on the surface of target microbes, creating flexible heteropolymers that precisely replicate microbial surface structures. This method exhibits high affinity (KD = 2.7×108 CFU/mL for E. coli) and selectivity at the strain level. MIPs offer significant advantages over traditional antibodies, including greater stability, cost-effectiveness, and a broader spectrum of binding capabilities, making them effective for identifying and targeting various microbial strains, including unidentified or drug-resistant variants. Moreover, their favorable biocompatibility and functional resilience in diverse environments position MIPs as promising candidates for rapid pathogen detection and therapeutic applications. This research paves the way for advanced biomimetic materials in microbe-specific diagnostics and combating infections, addressing the critical need for effective tools in antibiotic resistance surveillance. © 2025
Keyword ：
Affinity Affinity Antibiotic resistance Antibiotic resistance Antibody mimics Antibody mimics Microbe-imprinted polymers Microbe-imprinted polymers Microbial recognition Microbial recognition
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| GB/T 7714 | Wu, Y. , Zhou, K. , Li, W. et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition [J]. | Chemical Engineering Journal , 2025 , 512 . |
| MLA | Wu, Y. et al. "Microbe-imprinted polymers for rapid drug-resistant bacteria recognition" . | Chemical Engineering Journal 512 (2025) . |
| APA | Wu, Y. , Zhou, K. , Li, W. , Huan, M. , Yu, Z. , Yan, F. et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition . | Chemical Engineering Journal , 2025 , 512 . |
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Herein, we developed a platinum-copper nano-enzyme-linked immunosorbent assay (NLISA) based split diagnostic platform for the ultrasensitive detection of cardiac troponin I (cTnI). The PtCu nanozyme synthesized by one-pot synthesis exhibited ultra-high peroxidase-like activity (35.17 U mg−1), which was about 4.5 times higher than that of the unmodified Pt nanozyme (8.83 U mg−1). Due to the efficient peroxidase-like activity of the copper-platinum complexed nanozyme, transduction and sequential amplification of cTnI biological signals were achieved in combination with a liposome-embedded amplification strategy. The encapsulation efficiency was calculated by introducing a liposomal bilayer model, which showed that the introduction of a single liposomal molecule could amplify the signal up to 870-fold, thus promising a high sensitivity test. Notably, the dynamic response of cTnI was in the range of 0.1–5000 pg mL−1 with an ultra-low detection limit (0.048 pg mL−1). The developed NLISA analysis system provides a new way to discover efficient and sensitive alternatives to ELISA kits, which can meet the practical needs of community healthcare testing conditions and rapid testing in hospitals. © 2024 Elsevier B.V.
Keyword ：
Cardiology Cardiology Copper compounds Copper compounds Immunology Immunology Platinum compounds Platinum compounds
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| GB/T 7714 | Lou, Fangming , Zhang, Han , Wang, Yanwen et al. Liposome-embedded PtCu nanozymes for improved immunoassay of accurate myocardial infarction [J]. | Talanta , 2024 , 279 . |
| MLA | Lou, Fangming et al. "Liposome-embedded PtCu nanozymes for improved immunoassay of accurate myocardial infarction" . | Talanta 279 (2024) . |
| APA | Lou, Fangming , Zhang, Han , Wang, Yanwen , Wang, Shaojie , Li, Qunfang , Tang, Dianping . Liposome-embedded PtCu nanozymes for improved immunoassay of accurate myocardial infarction . | Talanta , 2024 , 279 . |
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Background: Measurement of endogenous cellular hydrogen peroxide (H2O2) can provide information on cellular status, and help to understand cellular metabolism and signaling processes, thus contributing to elucidation of disease mechanisms and new diagnostics/therapeutic approaches. Results: In this work, Pt–Cd bimetallic nanozyme was successfully prepared via the solvothermal synthetic method for sensitive detection of H2O2. The synthesized Pt–Cd bimetallic nanozyme could exhibited good electrochemical activity. Then, the materials were analyzed for the electrochemical properties and catalytic properties of H2O2 by cyclic voltammetry and chronoamperometry, respectively. Results indicated that the synthesized nanozyme had superior sensitivity (295 μA⸳mM−1⸳cm−2) and selectivity toward H2O2 with a detection limit of 0.21 μM. Further, the Pt–Cd bimetallic nanozyme displayed good electrochemical properties compared to platinum catalysts alone. The application was extended to determine the produced H2O2 from human hepatocellular carcinoma cells (HepG2) and normal hepatocyte (LO2) samples after ascorbic acid stimulation, thus enabling the early warning of cellular carcinogenesis. Significance: This strategy promises simple, rapid, inexpensive and effective electrochemical sensing and provides a new pathway for the synthesis of bimetallic nanozymes to construct an electrochemical sensor for the sensitive detection of H2O2. © 2024 Elsevier B.V.
Keyword ：
Enzyme-free electrochemical sensor Enzyme-free electrochemical sensor Hepatocellular carcinoma cells Hepatocellular carcinoma cells Intracellularly-originated hydrogen peroxide Intracellularly-originated hydrogen peroxide Pt–Cd bimetallic nanozyme Pt–Cd bimetallic nanozyme
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| GB/T 7714 | Xu, M. , Yu, Z. , Wei, Q. et al. Probing trace of intracellularly-originated hydrogen peroxide based Pt–Cd bimetallic nanozyme on an enzyme-free electrochemical sensor [J]. | Analytica Chimica Acta , 2024 , 1324 . |
| MLA | Xu, M. et al. "Probing trace of intracellularly-originated hydrogen peroxide based Pt–Cd bimetallic nanozyme on an enzyme-free electrochemical sensor" . | Analytica Chimica Acta 1324 (2024) . |
| APA | Xu, M. , Yu, Z. , Wei, Q. , Tang, D. . Probing trace of intracellularly-originated hydrogen peroxide based Pt–Cd bimetallic nanozyme on an enzyme-free electrochemical sensor . | Analytica Chimica Acta , 2024 , 1324 . |
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