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Antibiotics are widely used as drugs to treathuman and animaldiseases. However, the widespread use of antibiotics has induced environmental contamination by antibiotics and development of resistant bacteria. There thus a need for advanced methods to remove antibiotics, e.g. from natural waters. Here we used the concept thatantigens and antibodies bind specificallyto remove tetracycline. Magnetic Fe3O4-polyamidoamine(PAMAM)-antibody complexes were synthesized to remove tetracycline at optimum pH and temperature. Invitro cytotoxicity of tetracycline/treated solutions was evaluated in human skin fibroblasts (HSF) cells by using MTT dyeassay, along with flow cytometry study for cell cycle distributions and cell apoptosis. The results show that the toxicity of tetracycline solutions was highlyreduced after treatment by magnetic Fe3O4-PAMAM-antibody complexes, and the survival rate of the cells was increased. The tetracycline may induce the disintegration of the nucleosomes of the HSF cells and eventually lead to the cell apoptosis. The results show that the method of removing tetracycline has higher biosafety.
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ENVIRONMENTAL CHEMISTRY LETTERS
ISSN: 1610-3653
Year: 2019
Issue: 1
Volume: 17
Page: 543-549
5 . 9 2 2
JCR@2019
1 5 . 0 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
ESI HC Threshold:184
JCR Journal Grade:1
CAS Journal Grade:3
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