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Abstract:
Antibiotics are widely used as drugs to treat human and animal diseases. However, the widespread use of antibiotics has induced environmental contamination by antibiotics and development of resistant bacteria. There thus a need for advanced methods to remove antibiotics, e.g. from natural waters. Here we used the concept that antigens and antibodies bind specifically to remove tetracycline. Magnetic Fe 3 O 4 –polyamidoamine(PAMAM)–antibody complexes were synthesized to remove tetracycline at optimum pH and temperature. In vitro cytotoxicity of tetracycline/treated solutions was evaluated in human skin fibroblasts (HSF) cells by using MTT dye assay, along with flow cytometry study for cell cycle distributions and cell apoptosis. The results show that the toxicity of tetracycline solutions was highly reduced after treatment by magnetic Fe 3 O 4 –PAMAM–antibody complexes, and the survival rate of the cells was increased. The tetracycline may induce the disintegration of the nucleosomes of the HSF cells and eventually lead to the cell apoptosis. The results show that the method of removing tetracycline has higher biosafety. © 2018, Springer Nature Switzerland AG.
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Environmental Chemistry Letters
ISSN: 1610-3653
Year: 2019
Issue: 1
Volume: 17
Page: 543-549
5 . 9 2 2
JCR@2019
1 5 . 0 0 0
JCR@2023
ESI HC Threshold:184
JCR Journal Grade:1
CAS Journal Grade:3
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count: 8
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 0
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