Colloidal　quantum　dots　(QDs)　have　shown　great　promise　in　the　development　of　high-resolution　displays　for　near-eye　visual　communication　with　the　outside　world.　However,　achieving　full-color　high-precision　assembly　of　QDs　at　the　nanoscale　remains　a　critical　challenge.　This　study　proposes　a　novel　strategy　for　the　region-selective　assembly　of　QDs　for　achieving　ultra-high-resolution　light-emitting　devices.　This　approach　leverages　interface-assembled　ultrathin　surface　microstructures　to　create　specific　wettability　patterns,　guiding　the　selective　assembly　of　QDs　on　their　surfaces.　Ultimately,　an　ultra-high-resolution　36　599　pixels　per　inch　(PPI)　light-emitting　device　is　successfully　realized,　with　a　record-high　external　quantum　efficiency　(EQE)　of　18.74%.　The　addition　of　a　molecular　microstructure　enhances　the　fidelity　of　the　assembled　QDs　pattern,　providing　nearly　a　two-fold　increase　in　contrast　ratio　while　effectively　suppressing　device　leakage　current.　Additionally,　the　assembly　process　is　compatible　with　photo-crosslinking　technology,　resulting　in　a　full-color　light-emitting　device　with　an　EQE　of　11.01%.　Our　strategy　paves　a　simple　and　effective　way　for　advancements　in　high-performance　nano-display　technology.