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author:

Qiu, Minghao (Qiu, Minghao.) [1] | Ren, Yuqing (Ren, Yuqing.) [2] | Huang, Lumin (Huang, Lumin.) [3] | Zhu, Xueying (Zhu, Xueying.) [4] | Liang, Tikai (Liang, Tikai.) [5] | Li, Meijin (Li, Meijin.) [6] (Scholars:李梅金) | Tang, Dianping (Tang, Dianping.) [7] (Scholars:唐点平)

Indexed by:

EI Scopus SCIE

Abstract:

The proof-of-concept of sensitive electrochemical immunoassay for the quantitative monitoring of human epidermal growth factor receptor 2 (HER2) is reported. The assay is carried out on iron nitrogen-doped carbon (FeNC) nanozyme-modified screen-printed carbon electrode using chronoamperometry. Introduction of target HER2 can induce the sandwiched immunoreaction between anti-HER2 monoclonal antibody-coated microplate and biotinylated anti-HER2 polyclonal antibody. Thereafter, streptavidin-glucose oxidase (GOx) conjugate is bonded to the detection antibody. Upon addition of glucose, 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) is oxidized through the produced H2O2 with the assistance of GOx and FeNC nanozyme. The oxidized TMB is determined via chronoamperometry. Experimental results revealed that electrochemical immunosensing system exhibited good amperometric response, and allowed the detection of target HER2 as low as 4.5 pg/mL. High specificity and long-term stability are acquired with FeNC nanozyme-based sensing strategy. Importantly, our system provides a new opportunity for protein diagnostics.

Keyword:

Chronoamperometry Electrochemical immunoassay Human epidermal growth factor receptor 2 Iron nitrogen-doped carbon Nanozyme

Community:

  • [ 1 ] [Qiu, Minghao]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China
  • [ 2 ] [Ren, Yuqing]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China
  • [ 3 ] [Huang, Lumin]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China
  • [ 4 ] [Zhu, Xueying]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China
  • [ 5 ] [Liang, Tikai]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China
  • [ 6 ] [Li, Meijin]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China
  • [ 7 ] [Tang, Dianping]Fuzhou Univ, Dept Chem, Key Lab Analyt Sci Food Safety & Biol, MOE & Fujian Prov, Fuzhou 350108, Peoples R China

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Source :

MICROCHIMICA ACTA

ISSN: 0026-3672

Year: 2023

Issue: 10

Volume: 190

5 . 4

JCR@2023

5 . 4 0 0

JCR@2023

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 7

SCOPUS Cited Count: 4

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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