Electrochemical　real-time　PCR　(EC-rtPCR)　overcomes　several　shortcomings　of　fluorescence-based　real-time　PCR.　But　traditional　electrochemical　method　has　some　limitations　which　reduce　the　accuracy　and　efficiency　of　testing.　To　overcome　the　disadvantages　of　chronoamperometry　(CA)　we　report　a　novel　electrochemical　method　where　a　peak　current　is　quickly　generated　for　the　current　vs.　time　curve　by　changing　the　waveform　of　voltage　excitation　in　the　working　electrode.　In　particular,　we　derived　a　mathematical　model　to　illustrate　the　principle　of　this　method　and　it　can　also　be　used　to　demonstrate　that　the　peak　current　is　linear　with　regards　to　the　concentration　of　the　target　substance.　Moreover,　we　developed　a　device　with　an　improved　electrochemical　circuit　to　generate　the　voltage　excitation　and　detect　the　peak　automatically.　Finally,　the　device　was　used　to　study　the　electrochemical　behavior　of　K-3[Fe(CN)(6)].　It＇s　shown　that　the　method　has　a　better　signal　to　noise　ratio　and　higher　sensitivity　than　chronoamperometry.　The　obtained　peak　current　is　linear　with　regards　to　the　concentration　of　the　target　substance.