Developing　a　high-performance　modification　protocol　is　critical　for　efficiently　fabricating　affinity　monolith.　Herein,　by　using　2,4,6-trichloro-1,3,5-triazine　as　the　linker,　a　simple　triazine-bridged　approach　was　proposed　for　efficiently　fabricating　aptamer-grafted　polyhedral　oligomeric　silsesquioxane-polyethyleneimine　affinity　monolith　with　high　specificity　toward　ochratoxin　A.　Experimental　parameters,　column　characteristics　and　specificity　performances　of　the　resultant　affinity　monolith　were　investigated　in　detail.　Under　the　optimal　conditions,　2,4,6-trichloro-1,3,5-triazine　was　rapidly　grafted　on　the　polyamine　matrix,　and　effectively　applied　to　the　subsequent　bridge　linkage　of　aptamers.　It　was　simple　and　effective,　which　resulted　in　a　significant　decrease　of　modification　time,　excellent　properties　including　the　high　coverage　density　of　aptamer　up　to　1799 pmol/μL　and　sensitive　detection　of　ochratoxin　A　as　low　as　10　pg/mL　in　beer　samples.　This　protocol　provides　a　facile　approach　for　fabricating　aptamer-grafted　polyamine　affinity　monoliths　with　highly　selective　discrimination　performance.　©　2019　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim