In　this　work,　a　novel　molecular　imprinted　polymer　(MIP)　was　prepared　with　＂one-pot＂　process　using　acrylamide2-methylpropanesulfonic　acid　(AMPS)　as　the　functional　monomer　and　ochratoxin　A　(OTA)　as　the　template　molecule.　The　morphology　and　structure　of　the　resulted　MIP　were　characterized,　and　its　adsorption　performance　and　mechanism　for　the　analytes　were　also　investigated.　The　results　showed　that　the　recovery　of　MIP　for　OTA　was　84.8%,　while　that　of　non-imprinted　polymer　(NIP)　was　only　4.5%.　Moreover,　the　recovery　ratio　of　OTA　to　ochratoxin　B　(OTB)　on　MIP　was　74.4　at　pH　8.5,　even　though　OTB　content　was　100　times　as　much　as　OTA　content　in　the　mixture　of　mycotoxins.　The　limit　of　detection　of　this　method　based　on　MIP　combined　with　high　performance　liquid　chromatography-fluorescence　detection　(HPLC-FLD)　for　OTA　was　0.07　ng　mL(-1),　which　was　lower　than　2　ng　mL(-1)　from　the　European　Union　limit　for　beer.　The　spiked　recoveries　of　OTA　were　in　the　range　of　95.0　+/-　1.5%-97.1　+/-　3.1%,　which　showed　that　the　obtained　MIP　had　great　potential　to　be　applied　as　a　specific　adsorbing　material　for　mycotoxins　in　complex　samples.