Redox-active　magnetic　molecularly　imprinted　polymer　(mMIP)　nanospheres　were　first　synthesized　and　functionalized　with　streptomycin　templates　for　highly　efficient　electrochemical　determination　of　streptomycin　residues　(STR)　in　food　by　coupling　with　bioelectrocatalytic　reaction　of　enzymes　for　signal　amplification.　The　mMIP　nanospheres　were　synthesized　by　using　Au(III)-promoted　molecularly　imprinted　polymerization　with　STR　templates　on　magnetic　beads.　Based　on　extraction　of　template　molecules　from　the　mMIP　surface,　the　imprints　toward　STR　templates　were　formed.　The　assay　was　implemented　with　a　competitive-type　assay　format.　Upon　addition　of　streptomycin,　the　analyte　competed　with　glucose　oxidase-labeled　streptomycin　(GOX-STR)　for　molecular　imprints　on　the　mMIP　nanospheres.　With　the　increasing　streptomycin　in　the　sample,　the　conjugation　amount　of　GOX-STR　on　the　mMIP　nanospheres　decreased,　leading　to　the　change　in　the　bioelectrocatalytic　current　relative　to　glucose　system.　Under　optimal　conditions,　the　catalytic　current　was　proportional　to　STR　level　in　the　sample,　and　exhibited　a　dynamic　range　of　0.05-20　ng　mL(-1)　with　a　detection　limit　of　10　pg　mL(-1)　STR　(at　3s(B)).　Intra-　and　inter-assay　coefficients　of　variation　were　below　12%.　The　assayed　results　for　STR　spiked　samples　including　milk　and　honey　with　the　mMIP-based　sensor　were　received　a　good　accordance　with　the　results　obtained　from　the　referenced　high-performance　liquid　chromatography　(HPLC)　method.　(C)　2012　Elsevier　B.V.　All　rights　reserved.