We　have　designed　a　new　molecularly　imprinted　co-polymer　(MIP)　for　the　sensitive　detection　of　streptomycin　(STR)　in　food　using　enzymes　as　signal　amplification.　The　MIP　was　fabricated　via　co-polymerization　of　aniline　and　o-phenylenediamine　on　gold　substrate　in　the　presence　of　STR　as　template.　The　assay　is　based　on　competitive　binding　of　free　STR　and　glucose　oxidase-labeled　STR　(GOx-STR)　to　the　imprinters　on　the　MIP.　On　addition　of　glucose,　hydrogen　peroxide　is　formed　that　is　detected　by　differential　pulse　voltammetry.　Under　optimal　conditions,　the　decrease　of　the　catalytic　current　is　proportional　to　the　STR　concentration　in　the　range　from　0.01　to　10ngmL1,　with　a　detection　limit　(LOD)　of　7.0pgmL1　STR　(at　3sB).　Intra-　and　inter-assay　coefficients　of　variation　(CVs)　are＜10.5%.　The　system　was　further　validated　and　evaluated　with　STR-spiked　samples　including　honey　and　milk,　and　the　recovery　was　between　82　and　124.2%.