One-phase-low-pH　method　is　a　simple,　efficient　and　easy-to-use　biogrouting　method　for　biomineralization　based　on　an　Enzyme　Induced　Carbonate　Precipitation　(EICP)　process.　This　method　utilizes　the　low-pH　biotreatment　solution　(a　mixture　of　urease　solution　and　cementation　solution)　to　provide　a　lag　period　for　the　biomineralization　process,　allowing　the　biotreatment　solution　to　be　uniformly　distributed　within　the　soil　and　thereby　improving　the　uniform　distribution　of　calcium　carbonate.　The　existing　one-phase-low-pH　method　uses　a　low　pH　urease　solution　to　prepare　the　biotreatment　solution.　However,　long-term　exposure　to　a　low　pH　environment　may　result　in　a　decrease　in　activity　or　even　inactivation　of　urease,　which　is　not　conducive　to　the　practical　application　of　this　technology.　In　this　study,　a　modified　one-phase-low-pH　method　using　low　pH　cementation　solution　is　proposed.　Three　sets　of　tests,　including　urease　activity　durability　tests,　solution　tests,　and　sand　column　treatment　tests,　were　conducted　in　this　study　to　clarify　the　necessity　and　feasibility　of　the　modified　method.　The　test　results　showed　that　the　acidic　environment　accelerated　the　decrease　of　urease　activity　over　time.　This　phenomenon　would　be　more　pronounced　at　a　lower　pH,　and　urease　would　be　immediately　inactive　at　a　pH　lower　than　4.5.　Meanwhile,　a　high　chemical　concentration　would　also　lead　to　a　decrease　in　activity　or　even　inactivation　of　urease.　If　urease　is　active　and　the　initial　pH　of　the　biotreatment　solution　is　higher　than　4.5,　the　pH　of　the　biotreatment　solution　will　rapidly　rise　to　a　weakly　alkaline　state　and　enzyme-induced　carbonate　precipitation　can　occur.　A　biotreatment　solution　that　would　produce　relatively　uniform　biomineralization　can　be　prepared　by　using　cementation　solution　with　a　pH　range　of　1.25-3.5　and　bacterial　urease　solution　in　a　volume　ratio　of　1:1.　For　the　sand　column　with　relatively　uniform　biomineralization,　the　pH　of　the　cementation　solution　(or　the　initial　pH　of　the　biotreatment　solution)　has　a　negligible　effect　on　the　strength　enhancement　for　similar　calcium　carbonate　content.