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学者姓名:崔明娟
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Abstract :
酶诱导碳酸钙沉淀技术(EICP)是一种环保高效的土体加固技术,钙源的种类可能会影响其加固效果。为定量化分析钙源对EICP固化钙质砂的影响,本文开展了不同钙源(氯化钙、醋酸钙、乳酸钙、硝酸钙)和不同Ca
Keyword :
微观机理 微观机理 无侧限抗压强度 无侧限抗压强度 渗透性 渗透性 酶诱导碳酸钙沉淀技术(EICP) 酶诱导碳酸钙沉淀技术(EICP) 钙源 钙源 钙质砂 钙质砂
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GB/T 7714 | 姜启武 , 黄明 , 崔明娟 et al. 钙源和Ca [J]. | 工程地质学报 , 2025 , 33 (03) : 908-918 . |
MLA | 姜启武 et al. "钙源和Ca" . | 工程地质学报 33 . 03 (2025) : 908-918 . |
APA | 姜启武 , 黄明 , 崔明娟 , 宋卿 , 许凯 , 李爽 . 钙源和Ca . | 工程地质学报 , 2025 , 33 (03) , 908-918 . |
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Enzyme-induced carbonate precipitation (EICP) has emerged as an environment-friendly solution for soil improvement. As a composite material, it is challenging to determine the micromechanical properties of EICP-reinforced sand using common macromechanical tests. In this work, a systematic study was conducted to determine the micromechanical properties of EICP-reinforced sand. The development of the micromechanical properties obtained from indentations along the route of "sand particle-CaCO3-sand particle" was examined. The width of the interfacial transition zone (ITZ) in EICP-reinforced sand was investigated. The effect of the reaction environment on ductility (i.e., the ratio of elastic modulus over hardness) of CaCO3 was investigated. The experimental results have identified that the width of ITZ in EICP-reinforced sand ranges from 0 to 180 mu m, which is significantly influenced by the crystal crystallinity or crystal morphology of CaCO3. The presence of porous media (i.e., sand particles) leads to the decrease in impurity content in the crystal formation environment, resulting in the lower ductility of CaCO3 accordingly. The mean value of fracture toughness of CaCO3 precipitation was identified to be the lowest one among sand particles, CaCO3 precipitation, and sand particles-CaCO3 interface. The lowest fracture toughness of CaCO3 indicating the failure of biocementation is derived from the CaCO3-CaCO3 breakage.
Keyword :
Bio-geotechnics Bio-geotechnics Enzyme-induced carbonate precipitation (EICP) Enzyme-induced carbonate precipitation (EICP) Microindentation test Microindentation test Micromechanical properties Micromechanical properties Soil stabilization Soil stabilization
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GB/T 7714 | Xu, Kai , Huang, Ming , Cui, Mingjuan et al. Micromechanical properties and bonding fracture of EICP-reinforced sand analyzed using microindentation test [J]. | ACTA GEOTECHNICA , 2025 , 20 (7) : 3543-3561 . |
MLA | Xu, Kai et al. "Micromechanical properties and bonding fracture of EICP-reinforced sand analyzed using microindentation test" . | ACTA GEOTECHNICA 20 . 7 (2025) : 3543-3561 . |
APA | Xu, Kai , Huang, Ming , Cui, Mingjuan , Jin, Guixiao , Li, Shuang . Micromechanical properties and bonding fracture of EICP-reinforced sand analyzed using microindentation test . | ACTA GEOTECHNICA , 2025 , 20 (7) , 3543-3561 . |
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Biomineralization has been used for the treatment of calcareous sand to improve its properties. Although many studies have been performed on the biomineralized calcareous sand under freshwater conditions, few studies were focused on the behaviors of calcareous sand in seawater. As the freshwater is scarce in island areas, the freshwater-based biomineralization technology may be unsuitable for the treatment. In this study, seawater-based bacterial enzyme induced carbonate precipitation (BEICP) was proposed to treat calcareous sand. A series of tests were conducted to verify the feasibility and efficiency of this treatment method through investigating the effects of seawater on the biomineralization and the properties of biomineralized calcareous sand in comparison with microbially induced carbonate precipitation (MICP). Test results reveal that seawater leads to the decrease of urease activity of bacterial cells and urease. NaCl, MgCl2, Na2SO4, and CaCl2 are the main inhibitory components in seawater, of which MgCl2 and CaCl2 have a strong influence on the urease activity of bacterial cells and urease, respectively. Compared to MICP treatment, BEICP-treated calcareous sand exhibits higher unconfined compressive strength and better biomineralization effects. The findings of this study can contribute to the application of biomineralization technology in island areas. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.
Keyword :
Bacterial enzyme-induced carbonate precipitation Bacterial enzyme-induced carbonate precipitation Biomineralization Biomineralization Calcareous sand Calcareous sand Seawater Seawater Strength Strength
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GB/T 7714 | Cui, M.-J. , Wu, J.-B. , Lai, H.-J. et al. Seawater-based bacterial enzyme induced carbonate precipitation for biomineralization of calcareous sand [J]. | Acta Geotechnica , 2025 . |
MLA | Cui, M.-J. et al. "Seawater-based bacterial enzyme induced carbonate precipitation for biomineralization of calcareous sand" . | Acta Geotechnica (2025) . |
APA | Cui, M.-J. , Wu, J.-B. , Lai, H.-J. , Huang, M. , Zheng, J.-J. , Hu, X. et al. Seawater-based bacterial enzyme induced carbonate precipitation for biomineralization of calcareous sand . | Acta Geotechnica , 2025 . |
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Heavy metal pollution in landfill soil poses a dual challenge of environmental toxicity and resource depletion. Enzyme-induced carbonate precipitation (EICP) was systematically evaluated as a sustainable stabilization method for cadmium (Cd), lead (Pb), and chromium (Cr) under both solution- and soil-phase conditions. Laboratory-scale experiments demonstrated that EICP achieved over 80% removal efficiency for Cd, Pb, and copper (Cu) in solution-phase systems, while soil-phase trials focused on Cd, Pb, and Cr to simulate realistic field conditions. Optimal performance was achieved using a 1:1 molar ratio of soybean-derived urease (1.0 U/mL) to CaCl2 (0.5 M), with Cd stabilization reaching 91.5%. Vacuum-assisted filtration improved treatment uniformity by 29.2% in clay soils. X-ray diffraction identified crystalline otavite in Cd systems, while Pb and Cu were stabilized via surface adsorption. Sequential extraction confirmed that over 70% of Cd was transformed into carbonate-bound phases. Treated soils met TCLP leaching standards and reuse criteria, maintaining neutral pH (7.2-8.1) and low salinity. Compared to cement-based methods, EICP avoids CO2 release from calcination and fossil fuel use. Carbon in urea is retained as solid CaCO3, reducing emissions by 0.3-0.5 t CO2-eq per ton of soil. These findings support EICP as a scalable, low-carbon alternative for landfill soil remediation.
Keyword :
biomineralization biomineralization carbonate precipitation carbonate precipitation metal immobilization metal immobilization soil remediation soil remediation urease urease
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GB/T 7714 | Xu, Wangqing , Zheng, Junjie , Cui, Mingjuan et al. Enzyme-Induced Carbonate Precipitation for the Stabilization of Heavy Metal-Contaminated Landfill Soils: A Sustainable Approach to Resource Recovery and Environmental Remediation [J]. | SUSTAINABILITY , 2025 , 17 (10) . |
MLA | Xu, Wangqing et al. "Enzyme-Induced Carbonate Precipitation for the Stabilization of Heavy Metal-Contaminated Landfill Soils: A Sustainable Approach to Resource Recovery and Environmental Remediation" . | SUSTAINABILITY 17 . 10 (2025) . |
APA | Xu, Wangqing , Zheng, Junjie , Cui, Mingjuan , Lai, Hanjiang . Enzyme-Induced Carbonate Precipitation for the Stabilization of Heavy Metal-Contaminated Landfill Soils: A Sustainable Approach to Resource Recovery and Environmental Remediation . | SUSTAINABILITY , 2025 , 17 (10) . |
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Soil contamination by heavy metals presents substantial ecological and geotechnical risks, thereby demanding sustainable remediation strategies. Conventional approaches, including chemical stabilization and microbial-induced carbonate precipitation (MICP), are limited by high costs, ecological disturbances, and sensitivity to environmental stressors. A plant-derived urease-driven enzyme-induced carbonate precipitation (EICP) system was evaluated for immobilizing cadmium (Cd2(+)), lead (Pb2(+)), and zinc (Zn2(+)) in contaminated soils. Systematic screening revealed that jack bean and watermelon seed ureases are optimal catalysts for heavy metal sequestration, achieving efficiencies of 87.3% for Cd2 (+) , 91.5% for Pb2 (+) , and 76.4% for Zn2 (+) . These high efficiencies are attributed to their catalytic specificity and the retained enzymatic activity under environmental stress. Critical process parameters were fine-tuned through iterative experimentation, maintaining a urea-CaCl2 reaction stoichiometry of 1.5:1 molar ratio and calibrating the enzyme dosage to 1.2 U/g of soil matrix. This optimized operational range effectively promoted carbonate mineralization while preserving essential soil hydraulic properties, as evidenced by sustained permeability exceeding 10 (-) (5) cm/s throughout precipitation cycles. Durability assessments under simulated acid rain and freeze-thaw cycles demonstrated 82.5% retention of Cd2(+) and 92.7% retention of unconfined compressive strength, outperforming conventional lime and MICP treatments. X-ray diffraction analysis confirmed the presence of stable crystalline phases. Field validation confirmed that the EICP protocol can be feasibly scaled to real-world sites with operational costs averaging $52 per cubic meter, representing a 61% reduction compared to microbial-based treatments. This plant-based EICP approach offers a scalable and cost-effective solution for ecological restoration and geotechnical stabilization in contaminated soils, demonstrating significant potential for sustainable environmental management.
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GB/T 7714 | Xu, Wangqing , Zheng, Junjie , Lai, Hanjiang et al. Sustainable heavy metal immobilization in contaminated soils using plant-derived urease-driven biomineralization [J]. | PLOS ONE , 2025 , 20 (9) . |
MLA | Xu, Wangqing et al. "Sustainable heavy metal immobilization in contaminated soils using plant-derived urease-driven biomineralization" . | PLOS ONE 20 . 9 (2025) . |
APA | Xu, Wangqing , Zheng, Junjie , Lai, Hanjiang , Cui, Mingjuan . Sustainable heavy metal immobilization in contaminated soils using plant-derived urease-driven biomineralization . | PLOS ONE , 2025 , 20 (9) . |
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One-phase-low-pH method is a simple, efficient and easy-to-use biogrouting method for biomineralization based on an Enzyme Induced Carbonate Precipitation (EICP) process. This method utilizes the low-pH biotreatment solution (a mixture of urease solution and cementation solution) to provide a lag period for the biomineralization process, allowing the biotreatment solution to be uniformly distributed within the soil and thereby improving the uniform distribution of calcium carbonate. The existing one-phase-low-pH method uses a low pH urease solution to prepare the biotreatment solution. However, long-term exposure to a low pH environment may result in a decrease in activity or even inactivation of urease, which is not conducive to the practical application of this technology. In this study, a modified one-phase-low-pH method using low pH cementation solution is proposed. Three sets of tests, including urease activity durability tests, solution tests, and sand column treatment tests, were conducted in this study to clarify the necessity and feasibility of the modified method. The test results showed that the acidic environment accelerated the decrease of urease activity over time. This phenomenon would be more pronounced at a lower pH, and urease would be immediately inactive at a pH lower than 4.5. Meanwhile, a high chemical concentration would also lead to a decrease in activity or even inactivation of urease. If urease is active and the initial pH of the biotreatment solution is higher than 4.5, the pH of the biotreatment solution will rapidly rise to a weakly alkaline state and enzyme-induced carbonate precipitation can occur. A biotreatment solution that would produce relatively uniform biomineralization can be prepared by using cementation solution with a pH range of 1.25-3.5 and bacterial urease solution in a volume ratio of 1:1. For the sand column with relatively uniform biomineralization, the pH of the cementation solution (or the initial pH of the biotreatment solution) has a negligible effect on the strength enhancement for similar calcium carbonate content.
Keyword :
Biomineralization Biomineralization Enzyme induced carbonate precipitation (EICP) Enzyme induced carbonate precipitation (EICP) Low pH cementation solution Low pH cementation solution One-phase-low-pH biogrouting method One-phase-low-pH biogrouting method Urease activity Urease activity
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GB/T 7714 | Chen, Yi-Wei , Cui, Ming-Juan , Lai, Han-Jiang et al. Modified one-phase-low-pH EICP method using low-pH cementation solution for soil biomineralization [J]. | ACTA GEOTECHNICA , 2025 , 20 (8) : 4133-4146 . |
MLA | Chen, Yi-Wei et al. "Modified one-phase-low-pH EICP method using low-pH cementation solution for soil biomineralization" . | ACTA GEOTECHNICA 20 . 8 (2025) : 4133-4146 . |
APA | Chen, Yi-Wei , Cui, Ming-Juan , Lai, Han-Jiang , Zheng, Jun-Jie , Ren, Yu-Xiao . Modified one-phase-low-pH EICP method using low-pH cementation solution for soil biomineralization . | ACTA GEOTECHNICA , 2025 , 20 (8) , 4133-4146 . |
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Microbial-induced carbonate precipitation (MICP) technique has the potential to be an eco-friendly and sustainable solution for engineering problems. Despite the extensive amount of research that has been conducted recently on the MICP technique, there are few studies on the constitutive model of MICP-treated specimens. In this study, the statistical damage constitutive model of MICP-treated specimens was established based on the statistical theory and damage mechanics theory. The proposed model assumed that the microelement strength of biocemented sand follows the lognormal distribution and the Drucker-Prager criterion. The parameters S0 and F0 in the constitutive model were determined, and their physical significance was then discussed. The reasonableness of the proposed model was verified by comparing the theoretical results and the experimental results. The evolution of the damage variable (D), parameter S0, and parameter F0 with different calcium carbonate content (CCC) was analyzed. The statistical damage model based on the lognormal distribution was then compared with that based on the Weibull distribution. The results show that the parameter F0 and S0 can reflect the limiting strength and brittleness of MICP-treated specimens.. The specimens with higher cementation tend to have a higher accelerated damage rate. The damage variables eventually reach a stable value as the axial deformation increases. The proposed model can reflect the strain softening and strain hardening phenomena well, which can also represent the shear expansion and shear contraction characteristics of the volume strain curve. Overall, the research in this study can provide some theoretical support for the engineering application of MICP-treated specimens.
Keyword :
Calcium carbonate content Calcium carbonate content Damage intrinsic model Damage intrinsic model Drucker-Prager criterion Drucker-Prager criterion Lognormal distribution Lognormal distribution Microbial-induced carbonate precipitation (MICP) Microbial-induced carbonate precipitation (MICP)
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GB/T 7714 | Jiang, Qiwu , Huang, Ming , Xu, Kai et al. Statistical damage constitutive model of MICP-treated specimens based on lognormal distribution [J]. | ACTA GEOTECHNICA , 2025 , 20 (4) : 1759-1775 . |
MLA | Jiang, Qiwu et al. "Statistical damage constitutive model of MICP-treated specimens based on lognormal distribution" . | ACTA GEOTECHNICA 20 . 4 (2025) : 1759-1775 . |
APA | Jiang, Qiwu , Huang, Ming , Xu, Kai , Cui, Mingjuan , Li, Shuang , Jin, Guixiao . Statistical damage constitutive model of MICP-treated specimens based on lognormal distribution . | ACTA GEOTECHNICA , 2025 , 20 (4) , 1759-1775 . |
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An innovative microbial remediation protocol is proposed to overcome critical limitations of conventional microbial-induced carbonate precipitation (MICP) for concrete crack repair. The method integrates pH preconditioning of Sporosarcina pasteurii with a bioadditive-assisted crystallization strategy to address microbial inactivation under highly alkaline conditions, inefficient calcium utilization, and structural instability caused by metastable vaterite formation. Acidification to pH 5.5 preserved 78 % of urease activity at pH 12.5 by stabilizing bacterial zeta potential, while a composite bioadditive composed of polyvinyl alcohol, sodium alginate, and colloidal silica nanoparticles reduced the critical nucleation radius by 29 %, enhancing calcite crystal formation. Mechanical testing showed a 26.8 % increase in flexural strength and an 88.7 % calcium utilization rate, with durability evaluations confirming stable crack sealing over 180 thermal-humidity cycles. Field-scale application to a deteriorated underground garage demonstrated 92 % void-filling efficiency and compressive strength recovery from 28.5 MPa to 41.2 MPa. The developed protocol eliminates the need for carrier materials and reduces carbon emissions, establishing a scalable and sustainable framework for infrastructure rehabilitation. These results highlight the potential of synergistic biological and material strategies for advancing next-generation self-healing concrete technologies. © 2025
Keyword :
Bending strength Bending strength Bending tests Bending tests Biological materials preservation Biological materials preservation Carbon carbon composites Carbon carbon composites Compression testing Compression testing Compressive strength Compressive strength Cracks Cracks Fracture testing Fracture testing Hardness testing Hardness testing High performance concrete High performance concrete Self compacting concrete Self compacting concrete Self-healing materials Self-healing materials Tensile testing Tensile testing
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GB/T 7714 | Xu, Wangqing , Lai, Hanjiang , Cui, Mingjuan et al. Synergistic pH-bioadditive strategy for self-healing concrete: Achieving high-efficiency calcite crystallization and sustainable infrastructure rehabilitation [J]. | Construction and Building Materials , 2025 , 484 . |
MLA | Xu, Wangqing et al. "Synergistic pH-bioadditive strategy for self-healing concrete: Achieving high-efficiency calcite crystallization and sustainable infrastructure rehabilitation" . | Construction and Building Materials 484 (2025) . |
APA | Xu, Wangqing , Lai, Hanjiang , Cui, Mingjuan , Zheng, Junjie . Synergistic pH-bioadditive strategy for self-healing concrete: Achieving high-efficiency calcite crystallization and sustainable infrastructure rehabilitation . | Construction and Building Materials , 2025 , 484 . |
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Biomineralization based on bacterial enzyme induced carbonate precipitation (BEICP) process is a promising alternative to cement-based ground treatment technology. The bacterial urease used in BEICP process is usually ultrasonic extracted from urease-producing bacteria. To efficiently extract urease with relatively higher activity from bacterial cells, the ultrasonic extraction parameters of urease were optimized in this study. Next, a series of bacterial urease extraction tests and sand column treatment tests were conducted to investigate the effects of vibration amplitude, upper temperature limit, and cooling method on the urease extraction process and biomineralization of sand. The results show that the upper temperature limit is an important factor affecting the extraction efficiency and the activity of the extracted urease solution, and the optimum upper temperature limit is 50 degrees C. The results indicate that increasing vibration amplitude could improve the extraction efficiency, but it hardly affects the urease activity (UA) under the optimal temperature. Continuous cooling could effectively simplify the operation and further improve the efficiency of urease extraction. Under the same urease activity of biotreatment solution, there is no marked difference in calcium carbonate content (CCC) and unconfined compressive strength of biomineralized sand columns prepared by urease solution extracted with different vibration amplitudes and upper temperature limits. The results of this study could provide a reference for application of BEICP technology of urease extraction to large-scale soil treatment. (c) 2025 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
Keyword :
Bacterial enzyme induced carbonate Bacterial enzyme induced carbonate Biomineralization Biomineralization precipitation (BEICP) precipitation (BEICP) Soil improvement Soil improvement Ultrasound Ultrasound Urease extraction Urease extraction
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GB/T 7714 | Lai, Hanjiang , Chen, Yiwei , Cui, Mingjuan et al. Extraction of high activity bacterial urease and its application to biomineralization of soil [J]. | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2025 , 17 (3) : 1847-1861 . |
MLA | Lai, Hanjiang et al. "Extraction of high activity bacterial urease and its application to biomineralization of soil" . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING 17 . 3 (2025) : 1847-1861 . |
APA | Lai, Hanjiang , Chen, Yiwei , Cui, Mingjuan , Zheng, Junjie , Chen, Zhibo . Extraction of high activity bacterial urease and its application to biomineralization of soil . | JOURNAL OF ROCK MECHANICS AND GEOTECHNICAL ENGINEERING , 2025 , 17 (3) , 1847-1861 . |
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微生物诱导碳酸钙沉淀(MICP)是环境岩土工程领域一项新型的土体加固技术.微生物的生长及活性会受到外部磁场的影响,改变MICP中碳酸钙的晶型晶貌及沉淀方式,从而对碳酸钙的胶结性能产生影响.采用纳米四氧化三铁(Nano-Fe3O4),设计了Nano-Fe3O4作用下的微生物诱导碳酸钙沉淀的水溶液及MICP砂土固化试验,对比分析了Nano-Fe3O4含量对微生物诱导生成的碳酸钙晶体含量(CCC)、类型、比例以及MICP固化砂土力学强度等参数的影响规律,并结合扫描电镜(SEM)试验分析了溶液环境及砂柱中碳酸钙的微观形貌特征,系统归纳了Nano-Fe3O4对MICP的作用效果及机制.结果表明:(1)Nano-Fe3O4能够有效改善细菌的新陈代谢性能,显著提高细菌OD600及脲酶活性;(2)溶液环境中,MICP产生的碳酸钙晶体类型以球霰石为主,含少量方解石,且Nano-Fe3O4含量增加能够促进球霰石的生成及增大MICP沉淀物中稳定相碳酸钙所占的比例;(3)Nano-Fe3O4可以显著提高MICP固化砂土的无侧限抗压强度和CCC;(4)SEM分析结果表明,溶液环境中,碳酸钙晶体以球型堆积为主,MICP固化砂柱中碳酸钙晶型随Nano-Fe3O4含量的增加逐渐呈菱柱状堆积.
Keyword :
MICP固化砂土 MICP固化砂土 微生物诱导碳酸钙沉淀(MICP) 微生物诱导碳酸钙沉淀(MICP) 晶体类型与形貌 晶体类型与形貌 溶液环境 溶液环境 纳米四氧化三铁 纳米四氧化三铁 细菌脲酶活性 细菌脲酶活性
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GB/T 7714 | 李爽 , 黄明 , 崔明娟 et al. 纳米四氧化三铁对微生物诱导碳酸钙沉淀的作用效果与机理研究 [J]. | 材料导报 , 2024 , 38 (20) : 76-83 . |
MLA | 李爽 et al. "纳米四氧化三铁对微生物诱导碳酸钙沉淀的作用效果与机理研究" . | 材料导报 38 . 20 (2024) : 76-83 . |
APA | 李爽 , 黄明 , 崔明娟 , 胡鑫杭 , 许凯 , 姜启武 . 纳米四氧化三铁对微生物诱导碳酸钙沉淀的作用效果与机理研究 . | 材料导报 , 2024 , 38 (20) , 76-83 . |
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