The　versatile　trophic　adaptability　of　Chlorella　sorokiniana　has　garnered　significant　attention,　establishing　it　as　a　promising　alga　for　efficient　lutein　production.　In　this　study,　the　lutein　production　characteristics　of　C　.　sorokiniana　FZU60　were　compared　under　autotrophic,　mixotrophic,　and　heterotrophic　conditions.　The　results　demonstrated　that　glucose　supplementation　promoted　algal　cell　growth　but　concurrently　reduced　lutein　content.　In　contrast,　light　induction　was　essential　for　enhancing　lutein　synthesis.　Consequently,　two-stage　trophic　strategies　were　developed　to　simultaneously　facilitate　cell　growth　and　lutein　synthesis.　It　was　determined　that　optimal　lutein　production　could　be　achieved　by　employing　the　heterotrophy-mixotrophy　strategy　in　conjunction　with　white　light　at　500　mu　mol/m2/s　during　the　second　stage　(mixotrophic　stage).　Furthermore,　combining　the　heterotrophymixotrophy　strategy　with　fed-batch　operation　further　enhanced　the　efficacy　of　lutein　production　in　FZU60.　The　resulting　lutein　productivity　demonstrated　a　remarkable　365.1　%　improvement　over　the　heterotrophy　strategy　(Strategy　I)　and　an　80.8　%　increase　over　the　mixotrophy　strategy　(Strategy　III),　when　both　were　coupled　with　fed-　batch　operation.　These　findings　were　subsequently　validated　in　a　5　L　fermenter　where　FZU60　exhibited　exceptional　lutein　content,　yield,　and　productivity　reaching　6.14　mg/g,　285.02　mg/L,　and　44.95　mg/L/d,　respectively.　Overall,　this　study　successfully　develops　an　effective　cultivation　strategy　to　address　the　inherent　conflict　between　cell　growth　and　lutein　synthesis　in　algal　cells,　thereby　demonstrating　considerable　potential　as　an　industrial　pathway　for　efficient　lutein　production.