Co-production　of　multiple　compounds　is　an　efficient　approach　to　enhance　the　economic　feasibility　of　microalgae-based　metabolites　production.　In　this　study,　Chlorella　sorokiniana　FZU60　was　cultivated　under　different　bioprocess　strategies　to　enhance　the　co-production　of　lutein　and　protein.　Results　showed　that　both　lutein　and　protein　content　(7.72　and　538.06　mg/g,　respectively)　were　highest　at　the　onset　of　nitrogen　deficiency　under　batch　cultivation.　Semi-batch　III　strategy,　with　75%　microalgal　culture　replacement　by　fresh　medium,　obtained　similar　content,　productivity,　and　yield　of　lutein　and　protein　as　batch　cultivation,　demonstrating　that　it　can　be　used　for　stable　and　continuous　production.　Fed-batch　II　strategy,　feeding　with　1/3　modified　BG11　medium,　achieved　super-high　lutein　and　protein　yield　(28.81　and　1592.77　mg/L,　respectively),　thus　can　be　used　for　high-output　production.　Besides,　two-stage　strategy,　combining　light　intensity　shift　and　semi-batch　cultivation,　gained　extremely　high　lutein　and　protein　productivity　(15.31　and　1080.41　mg/L/day,　respectively),　thereby　is　a　good　option　for　high-efficiency　production.　Moreover,　the　fed-batch　II　and　two-stage　strategy　achieved　high-quality　lutein　and　protein,　thus　are　promising　for　the　co-production　of　lutein　and　protein　in　C.　sorokiniana　FZU60　for　commercial　application.