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学者姓名：陈剑锋
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Abstract ：
Chromochloris zofingiensis, capable of high-cell-density heterotrophic cultivation, is deemed a highly promising contender for producing astaxanthin. However, the relatively low astaxanthin content impedes the improvement of its production. Herein, the cultivation conditions for heterotrophic astaxanthin production were optimized in this study. An optimized medium was proposed with a culture temperature of 30 degrees C and an initial biomass concentration of 0.6 g/L. Additionally, a two-stage cultivation strategy, supplying glucose upon carbon depletion, was explored to enhance both biomass concentration and astaxanthin content. Furthermore, a continuous fed-batch cultivation strategy, maintaining the glucose concentration at around 5 g/L under nitrogen deficiency, was established in a 5 L fermenter. This approach resulted in an impressive astaxanthin content of 1.92 mg/g and an outstanding astaxanthin production of 238.83 mg/L, surpassing most reported results obtained solely through fed-batch operation under heterotrophic cultivation. The findings highlight the immense potential of the proposed cultivation strategy for commercial astaxanthin production using C . zofingiensis.
Keyword ：
Astaxanthin Astaxanthin Chromochloris zofingiensis Chromochloris zofingiensis Culture conditions Culture conditions Fed-batch strategy Fed-batch strategy Heterotrophic cultivation Heterotrophic cultivation
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| GB/T 7714 | Ma, Ruijuan , Ma, Xin , Qiao, Yongcui et al. Improved production of astaxanthin in heterotrophic Chromochloris zofingiensis through optimized culture conditions incorporating an efficient fed-batch strategy [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 86 . |
| MLA | Ma, Ruijuan et al. "Improved production of astaxanthin in heterotrophic Chromochloris zofingiensis through optimized culture conditions incorporating an efficient fed-batch strategy" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 86 (2025) . |
| APA | Ma, Ruijuan , Ma, Xin , Qiao, Yongcui , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng et al. Improved production of astaxanthin in heterotrophic Chromochloris zofingiensis through optimized culture conditions incorporating an efficient fed-batch strategy . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 86 . |
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二元醇(包括乙二醇、丙二醇和丁二醇等)在化工、医疗、生物以及农业等许多领域都有广泛的应用,市场需求量大.目前,二元醇主要以化石资源为原料制备,但是化石资源有限的储量和使用过程中存在环境污染的问题,寻找一条可持续的、绿色的二元醇生产途径受到了越来越多的关注.生物质资源是自然界中唯一可再生的有机碳资源,以生物质基多元醇为原料制备二元醇有望克服化石资源的短缺并实现绿色可持续性发展.本文综述了国内外生物质基多元醇(甘油、赤藓糖醇、木糖醇和山梨醇)催化转化制备二醇催化剂的最新进展,总结了近年来多元醇催化氢解制备二醇的催化类型(外源氢体系和原位氢源体系)、催化效率、反应溶剂、反应途径、催化机制和催化稳定性,并对其未来的发展进行了展望,以期为生物质基多元醇高效催化转化为二元醇提供参考.
Keyword ：
二醇 二醇 催化剂 催化剂 多元醇 多元醇 生物质 生物质
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| GB/T 7714 | 宋顺明 , 张敬雯 , 张良清 et al. 生物质基多元醇催化转化制备二醇 [J]. | 化工进展 , 2025 , 44 (1) : 228-252 . |
| MLA | 宋顺明 et al. "生物质基多元醇催化转化制备二醇" . | 化工进展 44 . 1 (2025) : 228-252 . |
| APA | 宋顺明 , 张敬雯 , 张良清 , 邱佳容 , 陈剑锋 , 曾宪海 . 生物质基多元醇催化转化制备二醇 . | 化工进展 , 2025 , 44 (1) , 228-252 . |
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The versatile trophic adaptability of Chlorella sorokiniana has garnered significant attention, establishing it as a promising alga for efficient lutein production. In this study, the lutein production characteristics of C . sorokiniana FZU60 were compared under autotrophic, mixotrophic, and heterotrophic conditions. The results demonstrated that glucose supplementation promoted algal cell growth but concurrently reduced lutein content. In contrast, light induction was essential for enhancing lutein synthesis. Consequently, two-stage trophic strategies were developed to simultaneously facilitate cell growth and lutein synthesis. It was determined that optimal lutein production could be achieved by employing the heterotrophy-mixotrophy strategy in conjunction with white light at 500 mu mol/m2/s during the second stage (mixotrophic stage). Furthermore, combining the heterotrophymixotrophy strategy with fed-batch operation further enhanced the efficacy of lutein production in FZU60. The resulting lutein productivity demonstrated a remarkable 365.1 % improvement over the heterotrophy strategy (Strategy I) and an 80.8 % increase over the mixotrophy strategy (Strategy III), when both were coupled with fed- batch operation. These findings were subsequently validated in a 5 L fermenter where FZU60 exhibited exceptional lutein content, yield, and productivity reaching 6.14 mg/g, 285.02 mg/L, and 44.95 mg/L/d, respectively. Overall, this study successfully develops an effective cultivation strategy to address the inherent conflict between cell growth and lutein synthesis in algal cells, thereby demonstrating considerable potential as an industrial pathway for efficient lutein production.
Keyword ：
Chlorella sorokiniana Chlorella sorokiniana Fed-batch operation Fed-batch operation Lutein Lutein Trophic mode Trophic mode Trophic transition Trophic transition
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| GB/T 7714 | Zhang, Zhen , Cai, Weixin , Ma, Ruijuan et al. Two-stage trophic strategy coupled with fed-batch operation for simultaneous enhancement of cell growth and lutein synthesis in Chlorella sorokiniana [J]. | BIOCHEMICAL ENGINEERING JOURNAL , 2025 , 215 . |
| MLA | Zhang, Zhen et al. "Two-stage trophic strategy coupled with fed-batch operation for simultaneous enhancement of cell growth and lutein synthesis in Chlorella sorokiniana" . | BIOCHEMICAL ENGINEERING JOURNAL 215 (2025) . |
| APA | Zhang, Zhen , Cai, Weixin , Ma, Ruijuan , Ho, Shih-Hsin , Xie, Youping , Chen, Jianfeng . Two-stage trophic strategy coupled with fed-batch operation for simultaneous enhancement of cell growth and lutein synthesis in Chlorella sorokiniana . | BIOCHEMICAL ENGINEERING JOURNAL , 2025 , 215 . |
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Chlorella sorokiniana is a promising candidate for lutein production. However, the diversified pigment composition results in a complex and costly extraction process. In this study, a chlorophyll-deficient mutant (MT03) of C. sorokiniana FZU60 was generated by chemical mutagenesis, exhibiting lutein as the primary pigment under heterotrophic conditions with higher content and production than the wild type (WT). Transcriptomic and biochemical composition analyses showed the decreased contents of carbohydrates, lipids, and chlorophylls in MT03, which might lead to the increased biosynthesis of lutein and protein. The inhibition of chlorophyll biosynthesis also triggered the enhanced heme biosynthesis, potentially promoting the functions of cytochrome P450 carotenoid hydroxylases and subsequently facilitating lutein biosynthesis in MT03. Sequencing analysis indicated that the mutation of G1106 to D1106 in MT03 CHLH might hinder the magnesium chelating reaction, leading to the deficiency in chlorophyll biosynthesis. These findings highlight the potential of MT03 as a promising strain for lutein-directed biosynthesis and provide a straightforward and cost-effective approach for enhancing microalgae-based lutein production.
Keyword ：
Chlorella sorokiniana Chlorella sorokiniana Chlorophyll-deficient mutant Chlorophyll-deficient mutant Lutein Lutein Magnesium chelatase Magnesium chelatase Transcriptomic analysis Transcriptomic analysis
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| GB/T 7714 | Xie, Youping , Lin, Wenjin , Zhang, Wen et al. Chlorophyll-deficient triggers heterotrophic lutein-directed biosynthesis in a novel mutant strain of Chlorella sorokiniana (Chlorophyta): Process characterization and underlying mechanism [J]. | AQUACULTURE , 2025 , 598 . |
| MLA | Xie, Youping et al. "Chlorophyll-deficient triggers heterotrophic lutein-directed biosynthesis in a novel mutant strain of Chlorella sorokiniana (Chlorophyta): Process characterization and underlying mechanism" . | AQUACULTURE 598 (2025) . |
| APA | Xie, Youping , Lin, Wenjin , Zhang, Wen , Meng, Xing , Ma, Ruijuan , Wang, Baobei et al. Chlorophyll-deficient triggers heterotrophic lutein-directed biosynthesis in a novel mutant strain of Chlorella sorokiniana (Chlorophyta): Process characterization and underlying mechanism . | AQUACULTURE , 2025 , 598 . |
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Haematococcus pluvialis has been used for commercial production of natural astaxanthin, yet the production cost is quite high due to the relatively low production efficiency. Exploring highly efficient cultivation strategies can be used to enhance the economic feasibility. In this study, an efficient nitrogen feeding strategy accompanied with high light induction was explored to enhance the biomass concentration and astaxanthin content in H. pluvialis QLD. The results demonstrated that 1 x NaNO3 was the best feeding source under high light conditions, and feeding two times (Fed-batch II) obtained the best astaxanthin productivity. The maximum values of astaxanthin content, production, and productivity were 65.62 mg/g, 430.29 mg/L, and 26.77 mg/L/d, respectively, under Fed-batch II, surpassing previous findings reported in most studies. Biochemical composition analysis revealed alterations in the biosynthesis of cellular metabolites under Fed-batch II, indicating the mitigation of stresses on microalgal cells and an enhancement of metabolic flux towards astaxanthin production. Moreover, gene expression analysis demonstrated an upregulation of astaxanthin biosynthesis during the late stage of Fed-batch II. The findings elucidate that the proposed fed-batch strategy can be employed for the highly efficient production of natural astaxanthin by H. pluvialis.
Keyword ：
Astaxanthin Astaxanthin Haematococcus pluvialis Haematococcus pluvialis High light induction High light induction Hyper-production Hyper-production Nitrogen feeding strategy Nitrogen feeding strategy
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| GB/T 7714 | Ma, Ruijuan , Tao, Xinyi , Wang, Baobei et al. Hyper-production of astaxanthin from Haematococcus pluvialis by a highly efficient nitrogen feeding strategy accompanied with high light induction [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 85 . |
| MLA | Ma, Ruijuan et al. "Hyper-production of astaxanthin from Haematococcus pluvialis by a highly efficient nitrogen feeding strategy accompanied with high light induction" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 85 (2025) . |
| APA | Ma, Ruijuan , Tao, Xinyi , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng , Xie, Youping . Hyper-production of astaxanthin from Haematococcus pluvialis by a highly efficient nitrogen feeding strategy accompanied with high light induction . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 85 . |
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To achieve the high-value utilization of squid skin obtained from squid processing, this study utilized an enzymatic approach to prepare protein peptides. The enzymatic hydrolysis parameters were optimized using single factor and response surface tests, with the concentration of peptide and trichloroacetic acid-nitrogen soluble index (TCA-NSI) serving as evaluation criteria. The amino acid composition, molecular weight distribution, peptide sequence, antioxidant activity, and in vitro simulated digestive properties of the prepared protein peptides were also thoroughly characterized. The results showed that the optimal conditions for enzymatic hydrolysis were as follows: Enzymatic temperature of 43.50 ℃, enzymatic time of 100 minutes, enzymatic pH of 7.50, trypsin to alkaline protease ratio of 2:1, and enzyme dosage of 4000 U/g. Under these specified conditions, the TCA-NSI achieved a value of 89.02%±0.66%. The prepared protein peptides exhibited a well-balanced amino acid composition, with an essential amino acid index (EAAI) value of 0.90. Furthermore, the prepared protein peptides encompassed a total of 43 polypeptide sequences, with 38 of these segments exhibiting molecular weights below 1800 Da. The IC50 values for scavenging DPPH radical, ABTS+ radical, and hydroxyl radical were determined to be 0.61, 0.28 and 1.95 mg/mL, respectively. Notably, the antioxidant activity remained favorable even after undergoing simulated gastrointestinal digestion in vitro. These findings serve to establish a solid theoretical basis for the advancement and exploitation of byproducts derived from squid processing, as well as their subsequent value-added utilization. © 2024 Editorial Department of Science and Technology of Food Science. All rights reserved.
Keyword ：
Alkalinity Alkalinity Amino acids Amino acids Antioxidants Antioxidants Enzymatic hydrolysis Enzymatic hydrolysis Molecular weight distribution Molecular weight distribution Peptides Peptides Physicochemical properties Physicochemical properties Shellfish Shellfish Trichloroacetic acid Trichloroacetic acid
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| GB/T 7714 | Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen et al. Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties [J]. | Science and Technology of Food Industry , 2024 , 45 (7) : 217-226 . |
| MLA | Qingqing, Zhu et al. "Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties" . | Science and Technology of Food Industry 45 . 7 (2024) : 217-226 . |
| APA | Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen , Youping, Xie . Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties . | Science and Technology of Food Industry , 2024 , 45 (7) , 217-226 . |
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为实现鱿鱼加工下脚料鱿鱼皮的高值化利用，本研究采用酶法制备鱿鱼皮蛋白肽。以多肽浓度和三氯乙酸可溶性氮指数（Trichloroacetic acid-nitrogen soluble index，TCA-NSI）为评价指标，采用单因素和响应面试验优化酶解工艺参数，并对所制备蛋白肽的氨基酸组成、分子量分布、蛋白肽序列、抗氧化活性及其体外模拟消化特性进行表征。结果表明，最佳酶解工艺条件为酶解温度43.50 ℃、酶解时间100 min、酶解pH7.50、胰蛋白酶与碱性蛋白酶比例2:1、加酶量4000 U/g，在此条件下其TCA-NSI可达89.02%±0.66%。所制备的鱿鱼皮蛋白肽其氨基酸组成均衡，必需氨基酸指数（Essential amino acid index，EAAI）可达0.90。此外，鱿鱼皮蛋白肽含有43个多肽序列，其中38个肽段的分子质量低于1800 Da，其DPPH自由基、ABTS+自由基和羟自由基清除率的IC50分别为0.61、0.28和1.95 mg/mL，且经体外模拟胃肠消化后仍能维持较好的抗氧化活性。本研究结果为鱿鱼加工下脚料的开发利用和精深加工提供了理论依据。
Keyword ：
体外模拟消化 体外模拟消化 抗氧化活性 抗氧化活性 理化性质 理化性质 蛋白肽 蛋白肽 鱿鱼皮 鱿鱼皮
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| GB/T 7714 | 朱清清 , 马瑞娟 , 陈剑锋 et al. 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 [J]. | 食品工业科技 , 2024 : 1-10 . |
| MLA | 朱清清 et al. "响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析" . | 食品工业科技 (2024) : 1-10 . |
| APA | 朱清清 , 马瑞娟 , 陈剑锋 , 谢友坪 . 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 . | 食品工业科技 , 2024 , 1-10 . |
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Polyethylene terephthalate (PET) is a polymer synthesized via the dehydration and condensation reaction between ethylene glycol and terephthalic acid. PET has emerged as one of the most extensively employed plastic materials due to its exceptional plasticity and durability. Nevertheless, PET has a complex structure and is extremely difficult to degrade in nature, causing severe pollution to the global ecological environment and posing a threat to human health. Currently, the methods for PET processing mainly include physical, chemical, and biological methods. Biological enzyme degradation is considered the most promising PET degradation method. In recent years, an increasing number of enzymes that can degrade PET have been identified, and they primarily target the ester bond of PET. This review comprehensively introduced the latest research progress in PET enzymatic degradation from the aspects of PET-degrading enzymes, PET biodegradation pathways, the catalytic mechanism of PET-degrading enzymes, and biotechnological strategies for enhancing PET-degrading enzymes. On this basis, the current challenges within the enzymatic PET degradation process were summarized, and the directions that need to be worked on in the future were pointed out. This review provides a reference and basis for the subsequent effective research on PET biodegradation.
Keyword ：
Biodegradation pathway Biodegradation pathway Bioengineering modification Bioengineering modification Catalytic mechanism Catalytic mechanism PET-degrading enzyme PET-degrading enzyme
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| GB/T 7714 | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate [J]. | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
| MLA | Qiu, Jiarong et al. "A comprehensive review on enzymatic biodegradation of polyethylene terephthalate" . | ENVIRONMENTAL RESEARCH 240 (2024) . |
| APA | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing , Wu, Jinzhi , Zeng, Xianhai , Shi, Xinguo et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate . | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
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Combination of taxa and function can provide a more comprehensive picture on human-induced microbial homogenization. Here, we obtained 2.58 billion high-throughput sequencing reads and 479 high-quality metagenome-assembled genomes (MAGs) of planktonic microbial communities in a subtropical river for 5 years. We found the microbial taxa homogenization and functional homogenization were uncoupled. Although human activities in downstream sites significantly decreased the taxonomic diversity of non-abundant ASV communities (16S rRNA gene amplicon sequence variants), they did not significantly decrease the taxonomic diversity of abundant ASV and total observed MAG communities. However, the total observed MAG communities in downstream sites tended to homogenize into some specific taxa which encode human-activity-related functional genes, such as nutrient cycles, greenhouse gas emission, antibiotic and arsenic resistance. Those specific MAGs with high taxonomic diversity caused the weak heterogenization of total observed MAG communities in downstream sites. Moreover, functional homogenization promoted the synchrony among downstream MAGs, and these MAGs constructed some specific network modules might to synergistically execute or resist the humanactivity-related functions. High synchrony also led to the tandem effects among MAGs and thus decreased community stability. Overall, our findings revealed the links of microbial taxa, functions and stability under human activity impacts, and provided a strong evidence to encourage us re-thinking biotic homogenization based on microbial taxa and their functional attributes.
Keyword ：
Biotic homogenization Biotic homogenization Human activities Human activities Metagenome Metagenome Microbial community Microbial community Nitrogen cycle Nitrogen cycle
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| GB/T 7714 | Liu, Lemian , Wang, Hongwei , Guo, Yisong et al. Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability [J]. | WATER RESEARCH , 2024 , 252 . |
| MLA | Liu, Lemian et al. "Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability" . | WATER RESEARCH 252 (2024) . |
| APA | Liu, Lemian , Wang, Hongwei , Guo, Yisong , Yan, Qi , Chen, Jianfeng . Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability . | WATER RESEARCH , 2024 , 252 . |
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Dinoflagellate blooms have negative adverse effects on marine ecosystems. However, our knowledge about the spatiotemporal distribution of dinoflagellate communities and their correlations with micro-eukaryotic and bacterial communities is still rare. Here, the sediment micro-eukaryotic and bacterial communities were explored in the Taiwan Strait (TWS) by 16S and 18S rRNA gene high-throughput sequencing. We found that the dinoflagellates were the most abundant algal group in TWS, and their relative abundance was higher in spring and autumn than in summer. Moreover, the species richness and community composition of dinoflagellates showed strong seasonal patterns. NO3-N and NH4-N had the strongest correlations with the spatiotemporal dynamics of community composition of dinoflagellates. The dinoflagellates had a significantly wider niche breadth than other algal groups for NH4-N, NO3-N and NO2-N, and therefore potentially contributed to a wider distribution range and high abundance in TWS. In addition, the dinoflagellates had stronger impacts on microeukaryotes than on bacteria for both community composition and species richness. However, the dinoflagellates showed close coexistence with bacteria but loose coexistence with microeukaryotes in spring co-occurrence networks. This close coexistence suggests the potentially strong synergy effects between dinoflagellates and bacteria in spring dinoflagellate blooms in TWS. Overall, this study revealed the distribution mechanisms of dinoflagellates in TWS based on niche breadth and also unveiled the different effects of dinoflagellates on micro-eukaryotic and bacterial communities.
Keyword ：
Bacterial communities Bacterial communities Dinoflagellate Dinoflagellate Harmful algal bloom Harmful algal bloom Micro-eukaryotic community Micro-eukaryotic community Niche breadth Niche breadth
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| GB/T 7714 | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe et al. Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities [J]. | MARINE POLLUTION BULLETIN , 2024 , 208 . |
| MLA | Wang, Hongwei et al. "Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities" . | MARINE POLLUTION BULLETIN 208 (2024) . |
| APA | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe , Chen, Jianfeng . Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities . | MARINE POLLUTION BULLETIN , 2024 , 208 . |
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