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学者姓名：陈剑锋
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Abstract ：
Chromochloris zofingiensis, capable of high-cell-density heterotrophic cultivation, is deemed a highly promising contender for producing astaxanthin. However, the relatively low astaxanthin content impedes the improvement of its production. Herein, the cultivation conditions for heterotrophic astaxanthin production were optimized in this study. An optimized medium was proposed with a culture temperature of 30 degrees C and an initial biomass concentration of 0.6 g/L. Additionally, a two-stage cultivation strategy, supplying glucose upon carbon depletion, was explored to enhance both biomass concentration and astaxanthin content. Furthermore, a continuous fed-batch cultivation strategy, maintaining the glucose concentration at around 5 g/L under nitrogen deficiency, was established in a 5 L fermenter. This approach resulted in an impressive astaxanthin content of 1.92 mg/g and an outstanding astaxanthin production of 238.83 mg/L, surpassing most reported results obtained solely through fed-batch operation under heterotrophic cultivation. The findings highlight the immense potential of the proposed cultivation strategy for commercial astaxanthin production using C . zofingiensis.
Keyword ：
Astaxanthin Astaxanthin Chromochloris zofingiensis Chromochloris zofingiensis Culture conditions Culture conditions Fed-batch strategy Fed-batch strategy Heterotrophic cultivation Heterotrophic cultivation
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| GB/T 7714 | Ma, Ruijuan , Ma, Xin , Qiao, Yongcui et al. Improved production of astaxanthin in heterotrophic Chromochloris zofingiensis through optimized culture conditions incorporating an efficient fed-batch strategy [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 86 . |
| MLA | Ma, Ruijuan et al. "Improved production of astaxanthin in heterotrophic Chromochloris zofingiensis through optimized culture conditions incorporating an efficient fed-batch strategy" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 86 (2025) . |
| APA | Ma, Ruijuan , Ma, Xin , Qiao, Yongcui , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng et al. Improved production of astaxanthin in heterotrophic Chromochloris zofingiensis through optimized culture conditions incorporating an efficient fed-batch strategy . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 86 . |
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Haematococcus pluvialis has been used for commercial production of natural astaxanthin, yet the production cost is quite high due to the relatively low production efficiency. Exploring highly efficient cultivation strategies can be used to enhance the economic feasibility. In this study, an efficient nitrogen feeding strategy accompanied with high light induction was explored to enhance the biomass concentration and astaxanthin content in H. pluvialis QLD. The results demonstrated that 1 x NaNO3 was the best feeding source under high light conditions, and feeding two times (Fed-batch II) obtained the best astaxanthin productivity. The maximum values of astaxanthin content, production, and productivity were 65.62 mg/g, 430.29 mg/L, and 26.77 mg/L/d, respectively, under Fed-batch II, surpassing previous findings reported in most studies. Biochemical composition analysis revealed alterations in the biosynthesis of cellular metabolites under Fed-batch II, indicating the mitigation of stresses on microalgal cells and an enhancement of metabolic flux towards astaxanthin production. Moreover, gene expression analysis demonstrated an upregulation of astaxanthin biosynthesis during the late stage of Fed-batch II. The findings elucidate that the proposed fed-batch strategy can be employed for the highly efficient production of natural astaxanthin by H. pluvialis.
Keyword ：
Astaxanthin Astaxanthin Haematococcus pluvialis Haematococcus pluvialis High light induction High light induction Hyper-production Hyper-production Nitrogen feeding strategy Nitrogen feeding strategy
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| GB/T 7714 | Ma, Ruijuan , Tao, Xinyi , Wang, Baobei et al. Hyper-production of astaxanthin from Haematococcus pluvialis by a highly efficient nitrogen feeding strategy accompanied with high light induction [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 85 . |
| MLA | Ma, Ruijuan et al. "Hyper-production of astaxanthin from Haematococcus pluvialis by a highly efficient nitrogen feeding strategy accompanied with high light induction" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 85 (2025) . |
| APA | Ma, Ruijuan , Tao, Xinyi , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng , Xie, Youping . Hyper-production of astaxanthin from Haematococcus pluvialis by a highly efficient nitrogen feeding strategy accompanied with high light induction . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2025 , 85 . |
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The versatile trophic adaptability of Chlorella sorokiniana has garnered significant attention, establishing it as a promising alga for efficient lutein production. In this study, the lutein production characteristics of C . sorokiniana FZU60 were compared under autotrophic, mixotrophic, and heterotrophic conditions. The results demonstrated that glucose supplementation promoted algal cell growth but concurrently reduced lutein content. In contrast, light induction was essential for enhancing lutein synthesis. Consequently, two-stage trophic strategies were developed to simultaneously facilitate cell growth and lutein synthesis. It was determined that optimal lutein production could be achieved by employing the heterotrophy-mixotrophy strategy in conjunction with white light at 500 mu mol/m2/s during the second stage (mixotrophic stage). Furthermore, combining the heterotrophymixotrophy strategy with fed-batch operation further enhanced the efficacy of lutein production in FZU60. The resulting lutein productivity demonstrated a remarkable 365.1 % improvement over the heterotrophy strategy (Strategy I) and an 80.8 % increase over the mixotrophy strategy (Strategy III), when both were coupled with fed- batch operation. These findings were subsequently validated in a 5 L fermenter where FZU60 exhibited exceptional lutein content, yield, and productivity reaching 6.14 mg/g, 285.02 mg/L, and 44.95 mg/L/d, respectively. Overall, this study successfully develops an effective cultivation strategy to address the inherent conflict between cell growth and lutein synthesis in algal cells, thereby demonstrating considerable potential as an industrial pathway for efficient lutein production.
Keyword ：
Chlorella sorokiniana Chlorella sorokiniana Fed-batch operation Fed-batch operation Lutein Lutein Trophic mode Trophic mode Trophic transition Trophic transition
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| GB/T 7714 | Zhang, Zhen , Cai, Weixin , Ma, Ruijuan et al. Two-stage trophic strategy coupled with fed-batch operation for simultaneous enhancement of cell growth and lutein synthesis in Chlorella sorokiniana [J]. | BIOCHEMICAL ENGINEERING JOURNAL , 2025 , 215 . |
| MLA | Zhang, Zhen et al. "Two-stage trophic strategy coupled with fed-batch operation for simultaneous enhancement of cell growth and lutein synthesis in Chlorella sorokiniana" . | BIOCHEMICAL ENGINEERING JOURNAL 215 (2025) . |
| APA | Zhang, Zhen , Cai, Weixin , Ma, Ruijuan , Ho, Shih-Hsin , Xie, Youping , Chen, Jianfeng . Two-stage trophic strategy coupled with fed-batch operation for simultaneous enhancement of cell growth and lutein synthesis in Chlorella sorokiniana . | BIOCHEMICAL ENGINEERING JOURNAL , 2025 , 215 . |
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二元醇(包括乙二醇、丙二醇和丁二醇等)在化工、医疗、生物以及农业等许多领域都有广泛的应用,市场需求量大.目前,二元醇主要以化石资源为原料制备,但是化石资源有限的储量和使用过程中存在环境污染的问题,寻找一条可持续的、绿色的二元醇生产途径受到了越来越多的关注.生物质资源是自然界中唯一可再生的有机碳资源,以生物质基多元醇为原料制备二元醇有望克服化石资源的短缺并实现绿色可持续性发展.本文综述了国内外生物质基多元醇(甘油、赤藓糖醇、木糖醇和山梨醇)催化转化制备二醇催化剂的最新进展,总结了近年来多元醇催化氢解制备二醇的催化类型(外源氢体系和原位氢源体系)、催化效率、反应溶剂、反应途径、催化机制和催化稳定性,并对其未来的发展进行了展望,以期为生物质基多元醇高效催化转化为二元醇提供参考.
Keyword ：
二醇 二醇 催化剂 催化剂 多元醇 多元醇 生物质 生物质
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| GB/T 7714 | 宋顺明 , 张敬雯 , 张良清 et al. 生物质基多元醇催化转化制备二醇 [J]. | 化工进展 , 2025 , 44 (1) : 228-252 . |
| MLA | 宋顺明 et al. "生物质基多元醇催化转化制备二醇" . | 化工进展 44 . 1 (2025) : 228-252 . |
| APA | 宋顺明 , 张敬雯 , 张良清 , 邱佳容 , 陈剑锋 , 曾宪海 . 生物质基多元醇催化转化制备二醇 . | 化工进展 , 2025 , 44 (1) , 228-252 . |
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Chlorella sorokiniana is a promising candidate for lutein production. However, the diversified pigment composition results in a complex and costly extraction process. In this study, a chlorophyll-deficient mutant (MT03) of C. sorokiniana FZU60 was generated by chemical mutagenesis, exhibiting lutein as the primary pigment under heterotrophic conditions with higher content and production than the wild type (WT). Transcriptomic and biochemical composition analyses showed the decreased contents of carbohydrates, lipids, and chlorophylls in MT03, which might lead to the increased biosynthesis of lutein and protein. The inhibition of chlorophyll biosynthesis also triggered the enhanced heme biosynthesis, potentially promoting the functions of cytochrome P450 carotenoid hydroxylases and subsequently facilitating lutein biosynthesis in MT03. Sequencing analysis indicated that the mutation of G1106 to D1106 in MT03 CHLH might hinder the magnesium chelating reaction, leading to the deficiency in chlorophyll biosynthesis. These findings highlight the potential of MT03 as a promising strain for lutein-directed biosynthesis and provide a straightforward and cost-effective approach for enhancing microalgae-based lutein production.
Keyword ：
Chlorella sorokiniana Chlorella sorokiniana Chlorophyll-deficient mutant Chlorophyll-deficient mutant Lutein Lutein Magnesium chelatase Magnesium chelatase Transcriptomic analysis Transcriptomic analysis
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| GB/T 7714 | Xie, Youping , Lin, Wenjin , Zhang, Wen et al. Chlorophyll-deficient triggers heterotrophic lutein-directed biosynthesis in a novel mutant strain of Chlorella sorokiniana (Chlorophyta): Process characterization and underlying mechanism [J]. | AQUACULTURE , 2025 , 598 . |
| MLA | Xie, Youping et al. "Chlorophyll-deficient triggers heterotrophic lutein-directed biosynthesis in a novel mutant strain of Chlorella sorokiniana (Chlorophyta): Process characterization and underlying mechanism" . | AQUACULTURE 598 (2025) . |
| APA | Xie, Youping , Lin, Wenjin , Zhang, Wen , Meng, Xing , Ma, Ruijuan , Wang, Baobei et al. Chlorophyll-deficient triggers heterotrophic lutein-directed biosynthesis in a novel mutant strain of Chlorella sorokiniana (Chlorophyta): Process characterization and underlying mechanism . | AQUACULTURE , 2025 , 598 . |
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Dinoflagellate blooms have negative adverse effects on marine ecosystems. However, our knowledge about the spatiotemporal distribution of dinoflagellate communities and their correlations with micro-eukaryotic and bacterial communities is still rare. Here, the sediment micro-eukaryotic and bacterial communities were explored in the Taiwan Strait (TWS) by 16S and 18S rRNA gene high-throughput sequencing. We found that the dinoflagellates were the most abundant algal group in TWS, and their relative abundance was higher in spring and autumn than in summer. Moreover, the species richness and community composition of dinoflagellates showed strong seasonal patterns. NO3-N and NH4-N had the strongest correlations with the spatiotemporal dynamics of community composition of dinoflagellates. The dinoflagellates had a significantly wider niche breadth than other algal groups for NH4-N, NO3-N and NO2-N, and therefore potentially contributed to a wider distribution range and high abundance in TWS. In addition, the dinoflagellates had stronger impacts on microeukaryotes than on bacteria for both community composition and species richness. However, the dinoflagellates showed close coexistence with bacteria but loose coexistence with microeukaryotes in spring co-occurrence networks. This close coexistence suggests the potentially strong synergy effects between dinoflagellates and bacteria in spring dinoflagellate blooms in TWS. Overall, this study revealed the distribution mechanisms of dinoflagellates in TWS based on niche breadth and also unveiled the different effects of dinoflagellates on micro-eukaryotic and bacterial communities. © 2024 Elsevier Ltd
Keyword ：
Abiotic Abiotic Anthropogenic Anthropogenic
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| GB/T 7714 | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe et al. Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities [J]. | Marine Pollution Bulletin , 2024 , 208 . |
| MLA | Wang, Hongwei et al. "Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities" . | Marine Pollution Bulletin 208 (2024) . |
| APA | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe , Chen, Jianfeng . Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities . | Marine Pollution Bulletin , 2024 , 208 . |
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[目的]探明肥料种类和施肥量对秋茄(Kandelia obovata)幼苗生长的影响以及土壤微生物的响应.[方法]选用 3种有机肥和 1种无机肥,以不施肥组(CK)为对照,设置了 4个施肥量梯度,测定秋茄幼苗株高、叶长、叶宽、叶片数增长量、叶绿素含量,并对土壤微生物群落进行 16S rRNA基因高通量测序.[结果]秋茄最适施肥条件是 9.74 g·kg-1 基质的尊龙牌蚓肥,120 d后株高、叶长、叶宽、叶数增长量比对照组分别增加 117.50%、51.15%、63.34%、178.57%.施有机肥、无机肥和对照组的土壤微生物群落组成差异明显,施有机肥土壤中有更多特有的微生物.施有机肥土壤微生物α多样性变化趋势与植物生长指标变化趋势正相关,而施无机肥的呈负相关.表明有机肥可能通过促进土壤微生物与植物协同的方式进而有助于植物生长.施有机肥提高了土壤潜在有益菌,如放线菌门(Actinobacteriota)和Nitrospira、Nocardioides、Limibaculum属丰度.[结论]施适量有机肥协同促进秋茄生长和土壤微生物多样性,而施无机肥对秋茄生长促进作用相对较小,并对土壤微生物多样性产生负面影响.
Keyword ：
土壤微生物群落 土壤微生物群落 施肥条件 施肥条件 秋茄 秋茄 红树植物 红树植物
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| GB/T 7714 | 李龙 , 张梅玲 , 刘乐冕 et al. 不同施肥条件对秋茄幼苗培育及土壤微生物的影响 [J]. | 福建农业学报 , 2024 , 39 (7) : 839-847 . |
| MLA | 李龙 et al. "不同施肥条件对秋茄幼苗培育及土壤微生物的影响" . | 福建农业学报 39 . 7 (2024) : 839-847 . |
| APA | 李龙 , 张梅玲 , 刘乐冕 , 郭毅松 , 陈剑锋 . 不同施肥条件对秋茄幼苗培育及土壤微生物的影响 . | 福建农业学报 , 2024 , 39 (7) , 839-847 . |
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The xZrO(2)/TiO2-PMo(y) catalysts were synthesized using a conventional impregnation method with TiO2 as the support. By simply adjusting the loading amount of Zr species and the addition of phosphomolybdic acid, the acid and basic properties of the catalysts were rationally modulated. The synthesized 30%ZrO2/TiO2-PMo(6) catalyst exhibited excellent Meerwein-Ponndorf-Verley (MPV) reduction performance, achieving a 90.8% yield of gamma-valerolactone (GVL) from levulinic acid (LA) at 180 degrees C within 3 h. The total acidity, Lewis and Br & oslash;nsted acid sites, and basic sites of the xZrO(2)/TiO2-PMo(y) catalysts were characterized and their effects on the MPV reduction of LA to GVL were detailed discussion. The deactivation of the 30%ZrO2/TiO2-PMo(6) catalyst was mainly due to the carbon deposition instead of leaching, and the catalytic performance could be restored through an effective regeneration method. Additionally, the 30%ZrO2/TiO2-PMo(6) catalyst demonstrated superior universality in the MPV reduction of methyl levulinate (ML), ethyl levulinate (EL), and butyl levulinate (BL) to GVL, with yields of 85.3%, 84.2%, and 83.7% for ML, EL, and BL, respectively. This study presents a sustainable method for the MPV reduction of LA to GVL and provides a potentially valuable reference for other reactions that involve the synergistic catalysis of Lewis and Br & oslash;nsted sites along with basic sites.
Keyword ：
Acid and basicity role Acid and basicity role Catalyst Catalyst Easily adjustable activity Easily adjustable activity gamma-Valerolactone gamma-Valerolactone Levulinic acid/esters Levulinic acid/esters Stable recyclability Stable recyclability
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| GB/T 7714 | Zhang, Liangqing , Zhou, Ben , Hong, Yonglin et al. Efficient transformation of levulinic acid/esters to γ-valerolactone via a durable catalyst with simply tunable acid-base sites [J]. | RENEWABLE ENERGY , 2024 , 236 . |
| MLA | Zhang, Liangqing et al. "Efficient transformation of levulinic acid/esters to γ-valerolactone via a durable catalyst with simply tunable acid-base sites" . | RENEWABLE ENERGY 236 (2024) . |
| APA | Zhang, Liangqing , Zhou, Ben , Hong, Yonglin , Wu, Qiaomei , Qiu, Jiarong , Chen, Jianfeng et al. Efficient transformation of levulinic acid/esters to γ-valerolactone via a durable catalyst with simply tunable acid-base sites . | RENEWABLE ENERGY , 2024 , 236 . |
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Given the current energy crisis and concerns regarding environmental pollution, biomass is considered as a sustainable energy source that can potentially replace fossil fuels, and extensive research of biomass conversion has been directed towards the catalytic transformation of biomass into valuable hexanol and hexanediol. Hexanol and hexanediol are regarded as highly industrially valuable C6 alcohols, with hexanediol playing a crucial role in polyester industry, and hexanol being considered as a suitable alternative fuel. This review provided a comprehensive overview of biomass-derived production of hexanol and hexanediol, considering various feedstocks and catalytic approaches. It systematically outlined recent advancements in the catalytic conversion of biomass-derived compounds, such as cellulose-based substrates, 5-hydroxymethylfurfural and its derivatives, as well as hexanedioic acid and its esters, into hexanol and hexanediol. The review also summarized the applications of 1,6-hexanediol in the catalytic synthesis of C6 compounds (ε -caprolactone, adipic acid, 6-hydroxycaproic acid, and hexanediamine). Furthermore, it provided an outlook on the future trends in catalytic hydrogenation for producing hexanol and hexanediol, aiming to offer theoretical guidance and valuable insights for the eco-friendly and sustainable production of C6 compounds. © 2024 Chemical Industry Press Co., Ltd.. All rights reserved.
Keyword ：
biomass-based biomass-based C6 compound C6 compound catalyst catalyst hexanediol hexanediol hexanol hexanol
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| GB/T 7714 | Hong, Z. , Zhou, B. , Qiu, J. et al. Biomass-based catalytic transformation for producing hexanol, hexanediol, and their derivatives; [生物质基催化转化制备己醇和己二醇及其衍生品] [J]. | Chemical Industry and Engineering Progress , 2024 , 43 (11) : 6091-6110 . |
| MLA | Hong, Z. et al. "Biomass-based catalytic transformation for producing hexanol, hexanediol, and their derivatives; [生物质基催化转化制备己醇和己二醇及其衍生品]" . | Chemical Industry and Engineering Progress 43 . 11 (2024) : 6091-6110 . |
| APA | Hong, Z. , Zhou, B. , Qiu, J. , Zhang, L. , Chen, J. , Wang, B. et al. Biomass-based catalytic transformation for producing hexanol, hexanediol, and their derivatives; [生物质基催化转化制备己醇和己二醇及其衍生品] . | Chemical Industry and Engineering Progress , 2024 , 43 (11) , 6091-6110 . |
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Polyethylene terephthalate (PET) is a polymer synthesized via the dehydration and condensation reaction between ethylene glycol and terephthalic acid. PET has emerged as one of the most extensively employed plastic materials due to its exceptional plasticity and durability. Nevertheless, PET has a complex structure and is extremely difficult to degrade in nature, causing severe pollution to the global ecological environment and posing a threat to human health. Currently, the methods for PET processing mainly include physical, chemical, and biological methods. Biological enzyme degradation is considered the most promising PET degradation method. In recent years, an increasing number of enzymes that can degrade PET have been identified, and they primarily target the ester bond of PET. This review comprehensively introduced the latest research progress in PET enzymatic degradation from the aspects of PET-degrading enzymes, PET biodegradation pathways, the catalytic mechanism of PET-degrading enzymes, and biotechnological strategies for enhancing PET-degrading enzymes. On this basis, the current challenges within the enzymatic PET degradation process were summarized, and the directions that need to be worked on in the future were pointed out. This review provides a reference and basis for the subsequent effective research on PET biodegradation.
Keyword ：
Biodegradation pathway Biodegradation pathway Bioengineering modification Bioengineering modification Catalytic mechanism Catalytic mechanism PET-degrading enzyme PET-degrading enzyme
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| GB/T 7714 | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate [J]. | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
| MLA | Qiu, Jiarong et al. "A comprehensive review on enzymatic biodegradation of polyethylene terephthalate" . | ENVIRONMENTAL RESEARCH 240 (2024) . |
| APA | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing , Wu, Jinzhi , Zeng, Xianhai , Shi, Xinguo et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate . | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
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