Biomineralization　based　on　bacterial　enzyme　induced　carbonate　precipitation　(BEICP)　process　is　a　promising　alternative　to　cement-based　ground　treatment　technology.　The　bacterial　urease　used　in　BEICP　process　is　usually　ultrasonic　extracted　from　urease-producing　bacteria.　To　efficiently　extract　urease　with　relatively　higher　activity　from　bacterial　cells,　the　ultrasonic　extraction　parameters　of　urease　were　optimized　in　this　study.　Next,　a　series　of　bacterial　urease　extraction　tests　and　sand　column　treatment　tests　were　conducted　to　investigate　the　effects　of　vibration　amplitude,　upper　temperature　limit,　and　cooling　method　on　the　urease　extraction　process　and　biomineralization　of　sand.　The　results　show　that　the　upper　temperature　limit　is　an　important　factor　affecting　the　extraction　efficiency　and　the　activity　of　the　extracted　urease　solution,　and　the　optimum　upper　temperature　limit　is　50　°C.　The　results　indicate　that　increasing　vibration　amplitude　could　improve　the　extraction　efficiency,　but　it　hardly　affects　the　urease　activity　(UA)　under　the　optimal　temperature.　Continuous　cooling　could　effectively　simplify　the　operation　and　further　improve　the　efficiency　of　urease　extraction.　Under　the　same　urease　activity　of　biotreatment　solution,　there　is　no　marked　difference　in　calcium　carbonate　content　(CCC)　and　unconfined　compressive　strength　of　biomineralized　sand　columns　prepared　by　urease　solution　extracted　with　different　vibration　amplitudes　and　upper　temperature　limits.　The　results　of　this　study　could　provide　a　reference　for　application　of　BEICP　technology　of　urease　extraction　to　large-scale　soil　treatment.　©　2024　Institute　of　Rock　and　Soil　Mechanics,　Chinese　Academy　of　Sciences