One　nature-based　soil　improvement　method　is　the　Enzyme　Induced　Carbonate　Precipitation　(EICP)　approach　using　the　crude　soy-bean　urease　solution.　This　study　aims　to　investigate　the　extraction　of　soybean　urease　using　different　contents　of　ethanol,　and　its　effect　on　the　cementation　of　EICP-treated　sand.　Crude　urease　enzyme　was　extracted　from　the　soybean　powder　using　the　grinding-extraction　method　with　distilled　water　containing　ethanol.　The　activity　of　crude　soybean　urease　extracted　with　various　ethanol　contents　was　studied.　Sand　columns　treated　using　the　crude　soybean　urease　were　tested　to　evaluate　the　calcium　carbonate　precipitation　and　unconfined　com-pressive　strength.　The　test　results　show　that　the　proposed　extraction　method　using　ethanol　can　produce　clearer　crude　urease　solution　from　soybean　powder.　The　urease　activity　and　turbidity　of　the　extracted　urease　enzyme　solution　are　highly　dependent　on　the　content　of　ethanol,　with　the　optimal　ethanol　content　of　20%-30%　(v/v)　for　the　preparation　of　100　g/L　crude　soybean　urease　solution.　The　extracted　relatively　clear　urease　solution　contributes　to　improve　the　uniformity　of　calcium　carbonate　distribution　and　thus　the　strength　of　the　EICP-treated　sand　column.　(c)　2023　The　Japanese　Geotechnical　Society.　Production　and　hosting　by　Elsevier　B.V.　This　is　an　open　access　article　under　the　CC　BY-NC-ND　license　(http://creativecommons.org/licenses/by-nc-nd/4.0/).