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author:

Zhang, Fang (Zhang, Fang.) [1] (Scholars:张芳) | Gao, Chenshan (Gao, Chenshan.) [2] | Bai, Linlin (Bai, Linlin.) [3] | Chen, Yiquan (Chen, Yiquan.) [4] | Liang, Shuying (Liang, Shuying.) [5] | Lv, Xucong (Lv, Xucong.) [6] (Scholars:吕旭聪) | Sun, Jinyuan (Sun, Jinyuan.) [7] | Wang, Shaoyun (Wang, Shaoyun.) [8]

Indexed by:

EI SCIE

Abstract:

Food allergy has been a serious public health problem around the world. Its prevention relies heavily on the effective avoidance of any contaminated food, making clear and accurate detection very important. LAMP is one of the most potent methods for allergen rapid detection. However, its current colorimetric readouts usually have low color contrast and narrow color variation range. Thus, here we proposed a strategy based on color evolution to enlarge the variation range as well as the contrast to improve its suitability for naked-eye observation. By simply blending two commonly used color change processes during amplification, a wider color variation window, and a near contrast color change, purple-to-green with a hues difference of 10 were obtained. Three important allergens (walnuts, hazelnuts, and peanuts) were tested with a comparable sensitivity towards fluo-rescent real-time LAMP. Its feasibility for practical use has also been studied. This simple but effective strategy provides a new idea for the colorimetric detection of LAMP amplicons and can be applied to various fields.

Keyword:

Color blending Color evolution Food allergen Loop-mediated isothermal amplification Visual detection

Community:

  • [ 1 ] [Zhang, Fang]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 2 ] [Gao, Chenshan]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 3 ] [Bai, Linlin]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 4 ] [Liang, Shuying]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 5 ] [Lv, Xucong]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 6 ] [Wang, Shaoyun]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 7 ] [Gao, Chenshan]Beijing Technol & Business Univ, Beijing Lab Food Qual & Safety, Beijing 100048, Peoples R China
  • [ 8 ] [Sun, Jinyuan]Beijing Technol & Business Univ, Beijing Lab Food Qual & Safety, Beijing 100048, Peoples R China
  • [ 9 ] [Chen, Yiquan]Nanan Hosp, Clin Lab, Nanan 362300, Peoples R China

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Source :

FOOD CHEMISTRY-X

ISSN: 2590-1575

Year: 2022

Volume: 13

6 . 1

JCR@2022

6 . 5 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:74

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 18

SCOPUS Cited Count: 6

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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