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author:

Zhang, Fang (Zhang, Fang.) [1] | Gao, Chenshan (Gao, Chenshan.) [2] | Bai, Linlin (Bai, Linlin.) [3] | Chen, Yiquan (Chen, Yiquan.) [4] | Liang, Shuying (Liang, Shuying.) [5] | Lv, Xucong (Lv, Xucong.) [6] | Sun, Jinyuan (Sun, Jinyuan.) [7] | Wang, Shaoyun (Wang, Shaoyun.) [8]

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EI

Abstract:

Food allergy has been a serious public health problem around the world. Its prevention relies heavily on the effective avoidance of any contaminated food, making clear and accurate detection very important. LAMP is one of the most potent methods for allergen rapid detection. However, its current colorimetric readouts usually have low color contrast and narrow color variation range. Thus, here we proposed a strategy based on color evolution to enlarge the variation range as well as the contrast to improve its suitability for naked-eye observation. By simply blending two commonly used color change processes during amplification, a wider color variation window, and a near contrast color change, purple-to-green with a hues difference of 10 were obtained. Three important allergens (walnuts, hazelnuts, and peanuts) were tested with a comparable sensitivity towards fluorescent real-time LAMP. Its feasibility for practical use has also been studied. This simple but effective strategy provides a new idea for the colorimetric detection of LAMP amplicons and can be applied to various fields. © 2021 The Author(s)

Keyword:

Allergens Blending Color Colorimetry

Community:

  • [ 1 ] [Zhang, Fang]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China
  • [ 2 ] [Gao, Chenshan]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China
  • [ 3 ] [Gao, Chenshan]Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing; 100048, China
  • [ 4 ] [Bai, Linlin]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China
  • [ 5 ] [Chen, Yiquan]Clinical Laboratory, Nanan Hospital, Nanan; 362300, China
  • [ 6 ] [Liang, Shuying]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China
  • [ 7 ] [Lv, Xucong]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China
  • [ 8 ] [Sun, Jinyuan]Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing; 100048, China
  • [ 9 ] [Wang, Shaoyun]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China

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Food Chemistry: X

Year: 2022

Volume: 13

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count: 12

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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