• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Wu, Hui (Wu, Hui.) [1] | Zhang, Xianjun (Zhang, Xianjun.) [2] | Wu, Baoqiang (Wu, Baoqiang.) [3] | Qian, Cheng (Qian, Cheng.) [4] | Zhang, Fang (Zhang, Fang.) [5] (Scholars:张芳) | Wang, Liu (Wang, Liu.) [6] | Ye, Zunzhong (Ye, Zunzhong.) [7] | Wu, Jian (Wu, Jian.) [8]

Indexed by:

EI Scopus SCIE

Abstract:

Herein, we developed a rapid method for detection of genetically modified soybean (GTS 40-3-2) products using loop-mediated isothermal amplification (LAMP). A crude 5-minute extraction method was established for DNA extraction from soybeans and soybean products. LAMP reaction for CaMV35S promoter was optimized and the fastest threshold time (Tt) was 14 min with 4 mM magnesium ions at 63 degrees C. A portable instrument was designed to perform real-time LAMP in the field. As little as 0.1% GM soybean, specifically, or 0.5% GM ingredients in Chinese traditional tofu could be detected in 30 min from sampling to results. We used this method to further assess other five soybean products to determine whether they contained transgenic ingredients and compared the results with those obtained using PCR, which suggested the proposed method was applicable for rapid detection of genetically modified soybean in food products.

Keyword:

Genetically modified Loop-mediated isothermal amplification On-site detection Portable instrument Soybean products

Community:

  • [ 1 ] [Wu, Hui]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China
  • [ 2 ] [Qian, Cheng]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China
  • [ 3 ] [Ye, Zunzhong]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China
  • [ 4 ] [Wu, Jian]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China
  • [ 5 ] [Wang, Liu]Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China
  • [ 6 ] [Zhang, Xianjun]Hangzhou An Yu Technol Co Ltd, Hangzhou 310021, Peoples R China
  • [ 7 ] [Wu, Baoqiang]Hangzhou An Yu Technol Co Ltd, Hangzhou 310021, Peoples R China
  • [ 8 ] [Zhang, Fang]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • [Ye, Zunzhong]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China;;[Wang, Liu]Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China

Show more details

Related Keywords:

Source :

FOOD CHEMISTRY

ISSN: 0308-8146

Year: 2020

Volume: 323

7 . 5 1 4

JCR@2020

8 . 5 0 0

JCR@2023

ESI Discipline: AGRICULTURAL SCIENCES;

ESI HC Threshold:116

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 20

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

Online/Total:126/10098356
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1