Bacterial　resistance　has　become　a　serious　global　health　issue　over　the　past　decades　due　to　the　misuse　and　abuse　of　antibiotics.　The　development　of　effective　antibacterial　drugs　with　a　new　antibacterial　mechanism　is　thus　very　critical.　At　present,　there　are　many　reports　on　the　antibacterial　properties　and　mechanisms　of　two-dimensional　materials.　Currently,　the　modification　of　g-C3N4　materials,　as　widely　used　two-dimensional　materials,　has　become　a　key　step　in　extending　their　potential　applications　in　the　field　of　antimicrobial　therapy.　In　the　present　work,　we　prepared　sulfur-doped　g-C3N4　nanosheets　(SCNNSs),　which　have　good　water　dispersibility　and　sharp　tips.　The　electrostatic　interaction　of　SCNNSs　with　Tetrastigma　hemsleyanum　Diels　&　Gilg＇s　polysaccharide-3　(THDG-3)　provides　a　new　strategy　that　can　improve　the　killing　efficiency　of　SCNNSs.　In　addition,　THDG-3　can　rapidly　inhibit　bacterial　proliferation　in　the　early　stage　of　administration.　Combined　with　the　antibacterial　activity　of　the　SCNNSs,　TPS/SCNNSs　can　inhibit　bacteria　for　a　long　time.　Scanning　electron　microscopy　(SEM)　observation　of　Escherichia　coli　(E.　coli)　after　administration　of　the　materials　revealed　that　the　bacterial　cells　were　ruptured　and　their　intracellular　contents　were　completely　separated　from　the　cell　membrane.　Therefore,　we　speculate　that　the　bactericidal　mechanism　of　the　TPS/SCNNSs　probably　involves　cell　membrane　damage.　In　summary,　TPS/　SCNNSs　achieve　fast,　long-term,　dual-function　bacteriostatic　properties.　(c)　2020　Published　by　Elsevier　B.V.