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学者姓名：王建
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Abstract ：
Combined the electrostatic interaction of the negatively charged gold nanorods (AuNRs) (as acceptor) and Ru(bpy)(3)(2+) (as donor), an electrochemiluminescence resonance energy transfer (ECL-RET) sensor was constructed and applied for the detection of organophosphorus pesticides (OPs). Negatively charged AuNRs were synthesized by modifying AuNRs with polystyrene sulfonate (PSS) firstly, which can bind to Ru(bpy)(3)(2+) through electrostatic interaction so that the luminophore was absorbed by the acceptor, the resonance energy transfer occurred and only low ECL signal had been detected. Thiocholine can be produced by the hydrolysis process of acetylthiocholine (ATCh) with the help of acetylcholinesterase (AChE), which can bond with PSS-modified AuNRs (PSS-AuNRs) through gold-sulfur interaction, this caused the releasing of the adsorbed Ru(bpy)(3)(2+) into the solution and resulting in the restoration of the ECL intensity. However, the activity of AChE was inhibited by OPs, and the recovery process of the ECL signal was thus suppressed as well. In this study, chlorpyrifos was chosen as model target, the results indicated that the correlation between the ECL intensity and the logarithm of chlorpyrifos concentration showed remarkable linearity across 1 ng/mL to 1 mg/mL, achieving a detection limit of 0.51 ng/mL. The proposed system has been utilized for detecting OPs in real samples with satisfied results.
Keyword ：
Acetylcholinesterase Acetylcholinesterase Electrochemiluminescence Electrochemiluminescence Gold nanorods Gold nanorods Organophosphorus pesticides Organophosphorus pesticides Resonance energy transfer Resonance energy transfer
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| GB/T 7714 | Li, Zixin , Lin, Zeyu , Chen, Lifen et al. Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+ [J]. | TALANTA , 2025 , 281 . |
| MLA | Li, Zixin et al. "Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+" . | TALANTA 281 (2025) . |
| APA | Li, Zixin , Lin, Zeyu , Chen, Lifen , Lin, Yue , Luo, Fang , Lin, Cuiying et al. Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)32+ . | TALANTA , 2025 , 281 . |
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Background: Bulk-type conductive metal-organic frameworks (c-MOFs) had been applied to modify the electrode and used in electrochemical sensing because of the high conductive properties. But which still suffer from low mass permeability, restricted active site exposure, and poor accessibility due to the coordination saturation at metal sites in the bulk-type c-MOF. Recent studies have demonstrated that transforming bulk-type MOFs into MOFs nanosheets (NSs) can maximize the exposure of active sites and mass transfer. However, c-MOF NSs have rarely been applied in electrochemical sensing. Results: This study presents NSs type c-MOF Cu3(benzenehexathiol) (CuBHT), synthesized using a simple sacrificial template method. CuBHT NSs were modified onto a glassy carbon electrode (GCE) to prepare CuBHT NSs/ GCE, which was then applied to sense the model target ascorbic acid (AA), the system exhibits high sensitivity of 1.521 mA mM-1 cm-2 and a wide linear range of 1-789 mu M, low detection limit of 0.46 mu M. The sensitivity is 1.90 times higher than that of bulk-type CuBHT nanoparticles (NPs) modified GCE, which can be attributed to the CuBHT NSs having more exposed Cu sites on their surfaces. CuBHT NSs/GCE was then used to monitor AA levels in human sweat during daily activities or exercise, and the results indicated high reliability compared to the vitamin C ASA kit method. Significance: The design of c-MOF CuBHT NSs/GCE lead to better performance in terms of sensitivity and low detection limit in AA sensing compared to bulk-type nanoparticles. The AA sensing mechanism based on CuBHT was investigated, and the sensing system was demonstrated by detecting AA in sweat. This work advances both the fundamental understanding and practical applications of c-MOF NSs in AA sensing.
Keyword ：
Ascorbic acid Ascorbic acid Conductive metal-organic frameworks Conductive metal-organic frameworks Electrochemistry Electrochemistry Mechanism Mechanism Nanosheets Nanosheets
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| GB/T 7714 | Yuan, Runhao , Zhong, Xiaolong , Sun, Weiming et al. Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode [J]. | ANALYTICA CHIMICA ACTA , 2025 , 1353 . |
| MLA | Yuan, Runhao et al. "Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode" . | ANALYTICA CHIMICA ACTA 1353 (2025) . |
| APA | Yuan, Runhao , Zhong, Xiaolong , Sun, Weiming , Wang, Jian , Huang, Chuanhui , Lin, Zhenyu et al. Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode . | ANALYTICA CHIMICA ACTA , 2025 , 1353 . |
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Real time detection of hydrogen sulfide (H2S) in deep brain regions is of great significance. Photoelectrochemical (PEC) biosensors hold promise for real-time monitoring but low sensitivity due to the use of small electrode restrained its application. In this study, a miniaturized sensor that integrates organic photoelectrochemical transistors (OPECTs) with optical fiber (OF) microelectrodes was designed for the first time, which can amplify the small PEC signal into large channel current change. A conductive gold layer and cuprous oxide nanoparticles (Cu2O NPs) were sequentially deposited onto the OF, followed by a Nafion polymer coating to enhance anti-interference capabilities. Cu2O reacts with H2S to generate Cu2S. The resulting Cu2O/Cu2S heterojunction induces changes in the PEC signal and channel current. Compared to a conventional PEC system, the OPECT sensor has higher sensitivity and lower detection limit, enabling monitoring of H2S concentration fluctuations within the rat brain, which demonstrates the approach's capability for highly sensitive in vivo biomarker detection.
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| GB/T 7714 | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7467-7475 . |
| MLA | Zeng, Yulan et al. "Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7467-7475 . |
| APA | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui , Luo, Fang , Qiu, Bin , Fu, Fengfu et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7467-7475 . |
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The persistence of T-2 toxin in food and feed matrices renders it a pervasive contaminant, impacting both human and animal health. Traditional detection methods suffer from cumbersome instrumentation and intricate procedures, rendering on-site detection of T-2 toxin unfeasible. Therefore, we have constructed a real-time detection method for T-2 toxin detection by employing a target-responsive DNA hydrogel in conjunction with potassium iodide starch test paper. This method integrates both colorimetric and distance-based signal outputs, offering a streamlined and effective approach for the on-site detection of T-2 toxin. The specific binding of the target to the aptamer in the DNA hydrogel results in the collapse of the hydrogels structure, which changes the viscosity of the system and released horseradish peroxidase in the hydrogel wrapped, and then produces blue-purple marks of different lengths on the starch iodide papers to achieve the specific detection of T-2 toxin. Under optimized conditions, the assay exhibits a detection range spanning from 10 ng/mL to 10 mg/mL for the toxin, with a detection limit of 12.83 ng/mL. The proposed method has been successfully applied for the detection of real corn samples with satisfied result. Such colorimetric-distance dual signal detection method offers notable advantages, including straightforward operation, clear signal interpretation, and practical utility. Its implementation enables rapid, on-the-spot detection of T-2 toxin, particularly beneficial in resource-limited regions and less developed countries.
Keyword ：
Point-of-care testing Point-of-care testing Starch iodide paper Starch iodide paper T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Deng, Ye , Lin, Jiarong , Wang, Jingxuan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper [J]. | MICROCHEMICAL JOURNAL , 2025 , 210 . |
| MLA | Deng, Ye et al. "Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper" . | MICROCHEMICAL JOURNAL 210 (2025) . |
| APA | Deng, Ye , Lin, Jiarong , Wang, Jingxuan , Lin, Yue , Luo, Fang , Weng, Zuquan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper . | MICROCHEMICAL JOURNAL , 2025 , 210 . |
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Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM-10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
Keyword ：
aflatoxin B1 aflatoxin B1 microchannel microchannel portablesensor portablesensor resistance resistance surface charge density surface charge density
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| GB/T 7714 | Cai, Huabin , Huang, Yanling , Lin, Yue et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout [J]. | ACS SENSORS , 2024 , 9 (1) : 494-501 . |
| MLA | Cai, Huabin et al. "Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout" . | ACS SENSORS 9 . 1 (2024) : 494-501 . |
| APA | Cai, Huabin , Huang, Yanling , Lin, Yue , Luo, Fang , Chen, Lifen , Guo, Longhua et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout . | ACS SENSORS , 2024 , 9 (1) , 494-501 . |
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The electrochemiluminescence (ECL) intensity can be regulated by ionic current passing through the microchannel, which broadened the regulation of the ECL sensors. But in the early reported sensors, the electrostatic repulsion and steric hindrance caused few targets to approach the interface of the microchannel driven by concentration difference, which reduced the detection efficiency and prolonged the detection period. In this study, different accumulation strategies, such as a positive electric field and different polarity electric fields, were designed to accumulate targets in the microchannel. The interaction of azide groups and hydrogen sulfide served as a research model. Hydrogen sulfide can react with the negatively charged azide groups in the microchannel surface to produce positively charged amino groups, decreasing the negative charge density of the microchannel and thus altering the ionic current and ECL intensity. The accumulation of hydrogen sulfide at the microchannel tip can increase the collision probability with azide groups to improve the detection efficiency, and the integration of accumulation and reaction can shorten the detection period to 28 min. The hydrogen sulfide concentration on the microchannel tip accumulated by applying different polarity electric fields was 22.3-fold higher than that accumulated by applying a positive electric field. The selected research model broadened the application range of a microchannel-based ECL sensor and confirmed the universality of the microchannel-based ECL sensor.
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| GB/T 7714 | Huang, Yanling , Cai, Huabin , Lin, Yue et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (13) : 5251-5257 . |
| MLA | Huang, Yanling et al. "Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy" . | ANALYTICAL CHEMISTRY 96 . 13 (2024) : 5251-5257 . |
| APA | Huang, Yanling , Cai, Huabin , Lin, Yue , Luo, Fang , Lin, Cuiying , Wang, Jian et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (13) , 5251-5257 . |
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Establishing an effective system to measure formaldehyde (HCHO) content in food is of great significance due to food safety concern. Inspired by the mechanism of HCHO-induced protein denaturation and its effect on ion/molecule transport in nanochannels, a bioinspired microchannel-based electrochemiluminescence (ECL) sensor was constructed for HCHO detection. Benefiting from the water solubility of HCHO, the molecules rapidly spread and enriched at the ethylenediamine (EDA) functionalized microchannel interface. The reaction between EDA and HCHO significantly increased the negative charge density, leading to enhanced electroosmotic flow (EOF). This enhancement resulted in ion concentration depletion at the microchannel tip and a corresponding decrease in ionic current and ECL intensity. The ECL intensity exhibited a linear dependence on the logarithm of HCHO concentration ranging from 1 pg mL(-1) to 100 ng mL(-1), with a detection limit of 0.26 pg mL(-1)(S/N = 3). The biosensor demonstrated high selectivity, successfully detecting HCHO in shrimp samples. The performance of the bioinspired sensor was confirmed through comparation with existing methods, showcasing its superior sensitivity and reliability. The bioinspired sensor provides robust technical support for HCHO detection, crucial for food safety monitoring. Additionally, the innovative combination of bionics and microchannel-based ECL technology broadens the application range of ECL sensors, marking a significant advancement in the field.
Keyword ：
Bioinspired Bioinspired Charge density Charge density Electrochemiluminescence Electrochemiluminescence Formaldehyde Formaldehyde Microchannel Microchannel
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| GB/T 7714 | Huang, Yanling , Cai, Huabin , Luo, Fang et al. Protein denaturation inspired microchannel-based electrochemiluminescence sensor for formaldehyde detection [J]. | BIOSENSORS & BIOELECTRONICS , 2024 , 267 . |
| MLA | Huang, Yanling et al. "Protein denaturation inspired microchannel-based electrochemiluminescence sensor for formaldehyde detection" . | BIOSENSORS & BIOELECTRONICS 267 (2024) . |
| APA | Huang, Yanling , Cai, Huabin , Luo, Fang , Chen, Lifen , Lin, Cuiying , Wang, Jian et al. Protein denaturation inspired microchannel-based electrochemiluminescence sensor for formaldehyde detection . | BIOSENSORS & BIOELECTRONICS , 2024 , 267 . |
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A cell-free RNA transcription system had been coupled with electrochemiluminescence (ECL) detection technology for the first time to develop an ascorbic acid (AA, acting as a model target) biosensor. The biosensor is composed of single-stranded DNA (ssDNA) sequences modified with alkynyl and azido groups, respectively, alongside an incomplete gene circuit framework. The addition of target AA and copper ions will cause the linkage of the two ssDNA sequences through a click chemistry reaction. This results in the subsequent reconstruction of a complete gene circuit. The reconstituted gene circuit, in conjunction with the T7 RNA polymerase, drives the transcription of substantial quantities of RNA. ssDNA labeled with ferrocene (Fc) (Fc-DNA) had been immobilized on a tris(2,2 '-bipyridyl) ruthenium(II) chloride hexahydrate-doped SiO2 nanoparticle (Ru@SiO2 NPs) modified electrode first. The quenching effect of Fc on Ru@SiO2 causes the low ECL detected. The transcribed RNA sequence assisted double-stranded specific nuclease (DSN) to cut the ssDNA-Fc and the ECL of the system was enhanced. Optimal experimental conditions reveal that the ECL signal exhibits a linear correlation with the logarithmic concentration of AA, spanning a detection range from 100 nM to 1 mM, with a detection limit of 45 nM. This innovative methodology expands the utility of a cell-free RNA transcription system within the realm of biosensing applications.
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| GB/T 7714 | Chen, Ming , Zeng, Hongfu , Luo, Fang et al. Electrochemiluminescence Biosensor for Ascorbic Acid Based on Target Transformation of Cell-Free RNA Transcription System and Duplex-Specific Nuclease-Assisted Recycling Amplification [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (44) : 17807-17813 . |
| MLA | Chen, Ming et al. "Electrochemiluminescence Biosensor for Ascorbic Acid Based on Target Transformation of Cell-Free RNA Transcription System and Duplex-Specific Nuclease-Assisted Recycling Amplification" . | ANALYTICAL CHEMISTRY 96 . 44 (2024) : 17807-17813 . |
| APA | Chen, Ming , Zeng, Hongfu , Luo, Fang , Huang, Yunjian , Lin, Cuiying , Wang, Jian et al. Electrochemiluminescence Biosensor for Ascorbic Acid Based on Target Transformation of Cell-Free RNA Transcription System and Duplex-Specific Nuclease-Assisted Recycling Amplification . | ANALYTICAL CHEMISTRY , 2024 , 96 (44) , 17807-17813 . |
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The accurate monitoring of sulfide ion concentrations is vital for ensuring food safety, but current detection methods are often cumbersome and confined to laboratories. This study introduces an innovative paper-based colorimetric sensor that leverages the peroxidase-like activity of cobalt tetrasulfonate phthalocyanine (CoTsPc) for rapid, sensitive, and selective sulfide ion quantification. The underlying mechanism relies on the interaction between cobalt and sulfide ions, modulating the catalytic efficiency of CoTsPc and triggering a visible color change when 3,3 ',5,5 '-tetramethylbenzidine (TMB) and hydrogen peroxide are present. By controlling solvent evaporation, the reaction kinetics are standardized, enabling reproducible and accurate colorimetric measurements. Experimental results show that adding sulfide ions to the TMB-H2O2 reaction system causes a gradual decrease in color intensity on the paper substrate. This change verifies effectiveness of the sensor, highlighting its potential for reliable sulfide ion detection in food safety applications, while providing a simple and portable alternative to conventional methods.
Keyword ：
3 ' 3 ' 5 ' -tetramethylbenzidine (TMB) 5 ' -tetramethylbenzidine (TMB) Cobalt tetrasulfonate phthalocyanine (CoTsPc) Cobalt tetrasulfonate phthalocyanine (CoTsPc) Colorimetric measurements Colorimetric measurements Hydrogen peroxide Hydrogen peroxide Peroxidase-mimicking activity Peroxidase-mimicking activity
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| GB/T 7714 | Lin, Lichao , Wu, Lusha , Wang, Yating et al. A rapid and sensitive paper-based sensor for sulfide ion detection in Chinese liquors [J]. | FOOD CHEMISTRY , 2024 , 465 . |
| MLA | Lin, Lichao et al. "A rapid and sensitive paper-based sensor for sulfide ion detection in Chinese liquors" . | FOOD CHEMISTRY 465 (2024) . |
| APA | Lin, Lichao , Wu, Lusha , Wang, Yating , Jiang, Zhou , Wang, Jian . A rapid and sensitive paper-based sensor for sulfide ion detection in Chinese liquors . | FOOD CHEMISTRY , 2024 , 465 . |
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Lead halide perovskites have garnered attention as promising electrochemiluminescence (ECL) emitters owing to their superior photophysical characteristics. However, their poor water stability severely restricts their application in aqueous media for ECL. In this study, inorganic perovskite CsPbBr3 was assembled in situ in the imine-linked covalent-organic framework (COF-LZU1) as a novel ECL emitter. The expansive surface area and robust hydrophobic architecture of COF-LZU1 not only improved the water stability of CsPbBr3 but also guaranteed its exceptional ECL performance. The novel composite nanoluminescent material was coated onto an indium tin oxide (ITO) electrode via spin-coating and calcination processes to serve as an electrochemiluminescence (ECL) platform. A sensor was developed by combining a DNA hydrogel target-induced release system with a platform using ascorbic acid (AA) as a coreactant and T-2 toxin as the target analyte model. This method achieved a detection limit as low as 3.56 fgmL(-1) and was successfully applied to the analysis of the T-2 toxin content in corn samples. This study offers a novel path for the advancement of perovskite-based ECL emitters and their utilization in aqueous environments within the ECL field.
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| GB/T 7714 | Li, Jiawen , Wu, Zhibin , Luo, Fang et al. Stable Halide Perovskite CsPbBr3 Nanocrystals Assisted by Covalent-Organic Frameworks for Electrochemiluminescence Analysis in an Aqueous Medium [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (42) : 16783-16792 . |
| MLA | Li, Jiawen et al. "Stable Halide Perovskite CsPbBr3 Nanocrystals Assisted by Covalent-Organic Frameworks for Electrochemiluminescence Analysis in an Aqueous Medium" . | ANALYTICAL CHEMISTRY 96 . 42 (2024) : 16783-16792 . |
| APA | Li, Jiawen , Wu, Zhibin , Luo, Fang , Lin, Zhenyu , Wang, Jian , Li, Rui et al. Stable Halide Perovskite CsPbBr3 Nanocrystals Assisted by Covalent-Organic Frameworks for Electrochemiluminescence Analysis in an Aqueous Medium . | ANALYTICAL CHEMISTRY , 2024 , 96 (42) , 16783-16792 . |
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