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学者姓名：翁祖铨
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Abstract ：
Zearalenone (ZEN) is a common contaminant in crops with serious food safety implications. By leveraging the etching effect of H2O2 on gold nanorods (Au NRs) and the catalysis of Fenton reaction, we have successfully developed two enzyme-linked immunosorbent assay (ELISA) kits with distinct principles. ZEN can be accurately quantified by both methods by measuring changes in the longitudinal surface plasmon resonance (LSPR) absorption peaks of Au NRs. Direct detection kit is simpler and faster, with a linear range of 0.2-4000 ng/mL, while indirect competitive kit is more complex and has a narrower linear range of 0.02-10 ng/mL. This study is expected to provide an effective analytical strategy for rapid screening and accurate monitoring of mold contaminants in food and agricultural products.In this study, a novel dual-mode ELISA system was developed for the detection of zearalenone (ZEN) using Au NRs and their longitudinal surface plasmon resonance (LSPR) properties. By integrating H2O2-mediated nano-etching and Fenton reaction catalysis, the system enables both direct (0.2-4000 ng/mL) and indirect competitive (0.02-10 ng/mL) detection modes. The main work includes: (1) replacing the traditional enzyme colorimetric signal with an LSPR shift for accurate UV-visible quantification and visual multicolor readout; (2) optimizing the nanoenzyme catalytic system for improved reaction sensitivity; (3) eliminating the need for enzyme-labeled secondary antibodies in direct mode, thereby reducing cost and improving stability. The system was validated on grain samples with recoveries of 93.1-107.1% and detection limits of 0.2 ng/mL (direct) and 0.02 ng/mL (indirect). The modular design of the method allows it to be used for the detection of other mycotoxins (e.g., aflatoxin, ochratoxin) and offers great potential for food safety screening, agricultural monitoring, and environmental assessment. This work provides a cost-effective, sensitive and versatile platform for mycotoxin analysis.
Keyword ：
Food safety Food safety Gold nanorods Gold nanorods Visual detection Visual detection Zearalenone Zearalenone
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| GB/T 7714 | Hu, Yuanlong , Chen, Jiamin , Wu, Sifang et al. A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods [J]. | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION , 2025 , 19 (9) : 6665-6676 . |
| MLA | Hu, Yuanlong et al. "A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods" . | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION 19 . 9 (2025) : 6665-6676 . |
| APA | Hu, Yuanlong , Chen, Jiamin , Wu, Sifang , Zhong, Yi , Ye, Jialin , Ye, Lingqing et al. A colorimetric immunoassay for visual detection of zearalenone based on the Fenton reaction on gold nanorods . | JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION , 2025 , 19 (9) , 6665-6676 . |
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The persistence of T-2 toxin in food and feed matrices renders it a pervasive contaminant, impacting both human and animal health. Traditional detection methods suffer from cumbersome instrumentation and intricate procedures, rendering on-site detection of T-2 toxin unfeasible. Therefore, we have constructed a real-time detection method for T-2 toxin detection by employing a target-responsive DNA hydrogel in conjunction with potassium iodide starch test paper. This method integrates both colorimetric and distance-based signal outputs, offering a streamlined and effective approach for the on-site detection of T-2 toxin. The specific binding of the target to the aptamer in the DNA hydrogel results in the collapse of the hydrogels structure, which changes the viscosity of the system and released horseradish peroxidase in the hydrogel wrapped, and then produces blue-purple marks of different lengths on the starch iodide papers to achieve the specific detection of T-2 toxin. Under optimized conditions, the assay exhibits a detection range spanning from 10 ng/mL to 10 mg/mL for the toxin, with a detection limit of 12.83 ng/mL. The proposed method has been successfully applied for the detection of real corn samples with satisfied result. Such colorimetric-distance dual signal detection method offers notable advantages, including straightforward operation, clear signal interpretation, and practical utility. Its implementation enables rapid, on-the-spot detection of T-2 toxin, particularly beneficial in resource-limited regions and less developed countries.
Keyword ：
Point-of-care testing Point-of-care testing Starch iodide paper Starch iodide paper T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Deng, Ye , Lin, Jiarong , Wang, Jingxuan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper [J]. | MICROCHEMICAL JOURNAL , 2025 , 210 . |
| MLA | Deng, Ye et al. "Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper" . | MICROCHEMICAL JOURNAL 210 (2025) . |
| APA | Deng, Ye , Lin, Jiarong , Wang, Jingxuan , Lin, Yue , Luo, Fang , Weng, Zuquan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper . | MICROCHEMICAL JOURNAL , 2025 , 210 . |
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Multifunctional hydrogels hold significant promise for promoting the healing of infected wounds but often fall short in inhibiting antibiotic-resistant pathogens, and their clinical translation is limited by complex preparation processes and high costs. In this study, a multifunctional hydrogel is developed by combining metal-phenolic networks (MPNs) formed by tannic acid (TA) and gallium ions (Ga3+) with chitosan (CS) through a simple one-step method. The resulting CS-TA-Ga3+ (CTG) hydrogel is cost-effective and exhibits desirable properties, including injectability, self-healing, pH responsiveness, hemostasis, antioxidant, anti-inflammatory, and antibacterial activities. Importantly, the CTG hydrogels are effective against antibiotic-resistant pathogens due to the unique antibacterial mechanism of Ga3+. In vivo studies demonstrate that the CTG hydrogel promotes follicle formation and collagen deposition, accelerating the healing of infected wounds by inhibiting blood loss, suppressing bacterial growth, and modulating the inflammatory microenvironment. These findings highlight the CTG hydrogel's potential as an advanced and translational dressing for enhancing the healing of infected wounds.
Keyword ：
antibacterial antibacterial gallium ions gallium ions infected wound healing infected wound healing metal-phenolic network metal-phenolic network multifunctional hydrogel multifunctional hydrogel
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| GB/T 7714 | Zou, Minglang , Chen, Cuiping , Wang, Mingda et al. Facile Fabrication of Injectable Multifunctional Hydrogels Based on Gallium-Polyphenol Networks with Superior Antibacterial Activity for Promoting Infected Wound Healing [J]. | ADVANCED HEALTHCARE MATERIALS , 2025 , 14 (9) . |
| MLA | Zou, Minglang et al. "Facile Fabrication of Injectable Multifunctional Hydrogels Based on Gallium-Polyphenol Networks with Superior Antibacterial Activity for Promoting Infected Wound Healing" . | ADVANCED HEALTHCARE MATERIALS 14 . 9 (2025) . |
| APA | Zou, Minglang , Chen, Cuiping , Wang, Mingda , Lei, Chen , Wang, Yongming , Luo, Fang et al. Facile Fabrication of Injectable Multifunctional Hydrogels Based on Gallium-Polyphenol Networks with Superior Antibacterial Activity for Promoting Infected Wound Healing . | ADVANCED HEALTHCARE MATERIALS , 2025 , 14 (9) . |
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The rise of antibiotic-resistant bacteria poses a serious global health threat, highlighting the urgent need for novel strategies beyond conventional antibiotic therapies. This study explores the potential of microbe-imprinted polymers (MIPs) as innovative, pathogen-specific affinity agents. Utilizing microbial surface-initiated polymerization, MIPs are in-situ synthesized on the surface of target microbes, creating flexible heteropolymers that precisely replicate microbial surface structures. This method exhibits high affinity (KD = 2.7x108 CFU/mL for E. coli) and selectivity at the strain level. MIPs offer significant advantages over traditional antibodies, including greater stability, cost-effectiveness, and a broader spectrum of binding capabilities, making them effective for identifying and targeting various microbial strains, including unidentified or drug-resistant variants. Moreover, their favorable biocompatibility and functional resilience in diverse environments position MIPs as promising candidates for rapid pathogen detection and therapeutic applications. This research paves the way for advanced biomimetic materials in microbe-specific diagnostics and combating infections, addressing the critical need for effective tools in antibiotic resistance surveillance.
Keyword ：
Affinity Affinity Antibiotic resistance Antibiotic resistance Antibody mimics Antibody mimics Microbe-imprinted polymers Microbe-imprinted polymers Microbial recognition Microbial recognition
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| GB/T 7714 | Wu, Yuanzi , Zhou, Kaiqiang , Li, Wenhui et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 512 . |
| MLA | Wu, Yuanzi et al. "Microbe-imprinted polymers for rapid drug-resistant bacteria recognition" . | CHEMICAL ENGINEERING JOURNAL 512 (2025) . |
| APA | Wu, Yuanzi , Zhou, Kaiqiang , Li, Wenhui , Huan, Min , Yu, Zhichao , Yan, Fen et al. Microbe-imprinted polymers for rapid drug-resistant bacteria recognition . | CHEMICAL ENGINEERING JOURNAL , 2025 , 512 . |
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The intracellular delivery of protein drugs via nanocarriers offers significant potential for expanding their therapeutic applications. However, the unintended activation of innate immune responses and inflammation triggered by the carriers presents a major challenge, often compromising therapeutic efficacy. Here, we present oligoethylenimine-thioketal (OEI-TK), a reactive oxygen species-responsive cationic polymer with intrinsic anti-inflammatory properties, to overcome this challenge. OEI-TK self-assembles electrostatically with bovine serum albumin (BSA) to form stable nanoparticles (OTB NPs) with excellent encapsulation efficiency. In vitro studies confirmed that OTB NPs retained OEI-TK's antioxidant and anti-inflammatory properties, enhanced biocompatibility, and efficiently delivered BSA into cells. Furthermore, OEI-TK facilitated the intracellular delivery of beta-galactosidase while preserving its enzymatic activity, demonstrating its potential for functional protein transport. These findings highlight OEI-TK as a promising platform with dual benefits of inflammation modulation and intracellular protein delivery, holding potential for the synergistic treatment of inflammation-related diseases.
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| GB/T 7714 | Wang, Yongming , Ming, Yangcan , Yu, Zhichao et al. ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery [J]. | BIOMACROMOLECULES , 2025 , 26 (4) : 2268-2281 . |
| MLA | Wang, Yongming et al. "ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery" . | BIOMACROMOLECULES 26 . 4 (2025) : 2268-2281 . |
| APA | Wang, Yongming , Ming, Yangcan , Yu, Zhichao , Xu, Zhenjin , Zou, Minglang , Chen, Cuiping et al. ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery . | BIOMACROMOLECULES , 2025 , 26 (4) , 2268-2281 . |
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Herein, we present an innovative electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of N-nitrosodimethylamine (NDMA). The biosensor utilizes a triple signal amplification strategy, combining rolling circle amplification (RCA), CRISPR/Cas12a-driven hyperbranched rolling circle amplification (HRCA), and electrostatic repulsion with size exclusion effects from vertically ordered mesoporous silica film (VMSF)/indium tin oxide (ITO) on double-stranded DNA (dsDNA)-Ru(phen)3 2+ complexes. In this system, aptamers and circular DNA undergo RCA reactions, followed by the CRISPR/Cas12a-mediated HRCA process, producing abundant dsDNA. The electropositive ECL indicator, namely Ru(phen)3 2+, was subsequently adsorbed onto the electronegative dsDNA, forming dsDNA-Ru(phen)3 2+ complexes. These complexes are subjected to electrostatic repulsion and size exclusion by the VMSF-modified ITO electrode, resulting in a lower ECL intensity. Upon introducing NDMA, the aptamer preferentially binds to NDMA, thereby preventing the formation of long dsDNA. This process releases free Ru(phen)3 2+, which diffuses to the electrode surface through narrow mesoporous channels via electrostatic adsorption. Consequently, an enhanced and strong ECL signal is observed. The integration of VMSF enhances selectivity and sensitivity by excluding larger impurities and promoting the electrostatic repulsion of dsDNA-Ru(phen)3 2+ complexes near the electrode surface. Additionally, the CRISPR/Cas12a system eliminates the formation of primer dimers and reduces false positives through its unique cis- and trans-cleavage activities. The biosensor demonstrated excellent performance with a linear correlation between the ECL signal and NDMA concentration in the range spanning from 10 pg/mL to 10 mu g/mL, achieving a low limit of detection of 5.33 pg/mL. This platform offers a reliable and robust solution for detecting NDMA in complex matrices, making it a promising tool for environmental monitoring, public health, and safety applications.
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| GB/T 7714 | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (10) : 5828-5835 . |
| MLA | Zheng, Zhenjie et al. "Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification" . | ANALYTICAL CHEMISTRY 97 . 10 (2025) : 5828-5835 . |
| APA | Zheng, Zhenjie , Qian, Zhuozhen , Huang, Da , Weng, Zuquan , Wang, Jian , Lin, Cuiying et al. Ultrasensitive Homogeneous Electrochemiluminescence Biosensor for N-Nitrosodimethylamine Detection Based on Vertically-Ordered Mesoporous Silica Film-Modified Electrode and CRISPR/Cas12a-Driven HRCA with Triple Signal Amplification . | ANALYTICAL CHEMISTRY , 2025 , 97 (10) , 5828-5835 . |
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In this study, we present the innovative design and comprehensive evaluation of a novel point-of-care testing (POCT) methodology for the rapid and accurate detection of T-2 toxin, a potent mycotoxin with significant implications for food safety and human health. The cornerstone of this approach lies in the integration of a copper-based conductive metal-organic framework (Cu3(HHTP)2) with a target-responsive DNA hydrogel system, creating a dual-signal readout mechanism that significantly enhances detection sensitivity and specificity. Specifically, the hydrogel covers the material's surface metal active sites, which become exposed upon target addition due to hydrogel collapse. The released Cu3(HHTP)2 can catalyze the oxidation of 3,3 ',5,5 '-tetrame-thylbenzidine, resulting in a colorimetric and temperature change in the solution. This dual-mode detection strategy enables both qualitative assessment through direct visual inspection of color change and quantitative analysis by monitoring the solution temperature variation post laser irradiation with a thermometer. Under the optimized conditions, the detection system demonstrates a wide range spanning from 5 to 200 ng/mL, with a detection limit of 1.67 ng/mL. This method has been successfully demonstrated through the analysis of real-world samples, yielding encouraging results that underscore its reliability and effectiveness. The proposed dual-signal approach boasts advantages such as straightforward operational procedures, unambiguous signal outputs, and robust practicality, making it an attractive option for mycotoxin detection in resource-constrained settings and developing regions.
Keyword ：
Cu3(HHTP)2 Cu3(HHTP)2 Point-of-care testing Point-of-care testing T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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| GB/T 7714 | Lin, Jiarong , Deng, Ye , Lin, Yue et al. Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 438 . |
| MLA | Lin, Jiarong et al. "Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel" . | SENSORS AND ACTUATORS B-CHEMICAL 438 (2025) . |
| APA | Lin, Jiarong , Deng, Ye , Lin, Yue , Luo, Fang , Weng, Zuquan , Wang, Jian et al. Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 438 . |
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Formaldehyde (FA), a known carcinogen, is occasionally used illegally as a preservative in seafood, while traditional detection methods for FA residues often fail to meet the practical needs for nondestructive detection. In this study, a approach was developed by combining a portable Raman spectrometer with the InceptionTime deep learning model without sample pretreatment. Model were trained by FA-negative and FA-positive Raman spectral data from the shrimp surface and achieved accuracies of 84.40 % and 85.17 % at detection thresholds of 5 mg/kg (the primary safety detection threshold) and 100 mg/kg (the abuse-level contamination threshold), respectively. Metabolomic analysis and weight visualization indicated that the model particularly focused on Raman peaks associated with specific amino acids and astaxanthin-binding proteins. Two amino acid metabolites, timonacic and spinacine, were also identified as direct indicators of FA addition. Our model offers a fielddeployable and practical approach for real-time and on-site FA detection scenario.
Keyword ：
Deep learning Deep learning Formaldehyde detection Formaldehyde detection Nondestructive detection Nondestructive detection Raman spectroscopy Raman spectroscopy Shrimp Shrimp
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| GB/T 7714 | Wei, Chencheng , Zhang, Jiheng , Li, Gaozheng et al. Rapid and non-destructive detection of formaldehyde adulteration in shrimp based on deep learning-assisted portable Raman spectroscopy [J]. | FOOD CHEMISTRY , 2025 , 492 . |
| MLA | Wei, Chencheng et al. "Rapid and non-destructive detection of formaldehyde adulteration in shrimp based on deep learning-assisted portable Raman spectroscopy" . | FOOD CHEMISTRY 492 (2025) . |
| APA | Wei, Chencheng , Zhang, Jiheng , Li, Gaozheng , Zhong, Yi , Ye, Zhaoting , Wang, Handong et al. Rapid and non-destructive detection of formaldehyde adulteration in shrimp based on deep learning-assisted portable Raman spectroscopy . | FOOD CHEMISTRY , 2025 , 492 . |
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The increasing prevalence of antibiotic resistance in Cutibacterium acnes (C. acnes) requires the search for alternative therapeutic strategies. Antimicrobial peptides (AMPs) offer a promising avenue for the development of new treatments targeting C. acnes. In this study, to design peptides with the specific inhibitory activity against C. acnes, we employed a deep learning pipeline with generators and classifiers, using transfer learning and pretrained protein embeddings, trained on publicly available data. To enhance the training data specific to C. acnes inhibition, we constructed a phylogenetic tree. A panel of 42 novel generated linear peptides was then synthesized and experimentally evaluated for their antimicrobial selectivity and activity. Five of them demonstrated their high potency and selectivity against C. acnes with MIC of 2-4 mu g/mL. Our findings highlight the potential of these designed peptides as promising candidates for anti-acne therapeutics and demonstrate the power of computational approaches for the rational design of targeted antimicrobial peptides.
Keyword ：
Antimicrobial peptides Antimicrobial peptides Cutibacterium acnes Cutibacterium acnes Deep learning Deep learning Pretrained protein language embedding Pretrained protein language embedding Transfer learning Transfer learning
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| GB/T 7714 | Dong, Qichang , Wang, Shaohua , Miao, Ying et al. Novel antimicrobial peptides against Cutibacterium acnes designed by deep learning [J]. | SCIENTIFIC REPORTS , 2024 , 14 (1) . |
| MLA | Dong, Qichang et al. "Novel antimicrobial peptides against Cutibacterium acnes designed by deep learning" . | SCIENTIFIC REPORTS 14 . 1 (2024) . |
| APA | Dong, Qichang , Wang, Shaohua , Miao, Ying , Luo, Heng , Weng, Zuquan , Yu, Lun . Novel antimicrobial peptides against Cutibacterium acnes designed by deep learning . | SCIENTIFIC REPORTS , 2024 , 14 (1) . |
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Curcumin (CUR) is a natural polyphenol that holds promise for treating ulcerative colitis (UC), yet oral administration of CUR exhibits limited bioavailability and existing formulations for oral delivery of CUR often suffer from unsatisfactory loading capacity. This study presents hydroxyethyl starch-curcumin microspheres (HCMSs) with excellent CUR loading capacity (54.52 %), and the HC-MSs can further encapsulate anti-inflammatory drugs dexamethasone (DEX) to obtain a combination formulation (DHC-MSs) with high DEX loading capacity (19.91 %), for combination therapy of UC. The microspheres were successfully engineered, retaining the antioxidative and anti-inflammatory activities of parental CUR and demonstrating excellent biocompatibility and controlled release properties, notably triggered by alpha-amylase, facilitating targeted drug delivery to inflamed sites. In a mouse UC model induced by dextran sulfate sodium, the microspheres effectively accumulated in inflamed colons and both HC-MSs and DHC-MSs exhibited superior therapeutic efficacy in alleviating UC symptoms compared to free DEX. Moreover, mechanistic exploration uncovered the multifaceted therapeutic mechanisms of these formulations, encompassing anti-inflammatory actions, mitigation of spleen enlargement, and modulation of gut microbiota composition. These findings underscore the potential of HC-MSs and DHC-MSs as promising formulations for UC, with implications for advancing treatment modalities for various inflammatory bowel disorders.
Keyword ：
Anti-inflammatory Anti-inflammatory Curcumin Curcumin Gut microbiota Gut microbiota Microspheres Microspheres Oral delivery Oral delivery Ulcerative colitis Ulcerative colitis
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| GB/T 7714 | Huang, Da , Wang, Yongming , Xu, Chenlan et al. Colon-targeted hydroxyethyl starch-curcumin microspheres with high loading capacity ameliorate ulcerative colitis via alleviating oxidative stress, regulating inflammation, and modulating gut microbiota [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 266 . |
| MLA | Huang, Da et al. "Colon-targeted hydroxyethyl starch-curcumin microspheres with high loading capacity ameliorate ulcerative colitis via alleviating oxidative stress, regulating inflammation, and modulating gut microbiota" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 266 (2024) . |
| APA | Huang, Da , Wang, Yongming , Xu, Chenlan , Zou, Minglang , Ming, Yangcan , Luo, Fang et al. Colon-targeted hydroxyethyl starch-curcumin microspheres with high loading capacity ameliorate ulcerative colitis via alleviating oxidative stress, regulating inflammation, and modulating gut microbiota . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 266 . |
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