Oviductus　ranae　(O.ran.)　has　been　widely　used　as　a　tonic　and　a　traditional　animal-based　Chinese　medicine.　O.ran.　extracts　have　been　reported　to　have　numerous　biological　activities,　including　activities　that　are　often　associated　with　mammalian　glycosaminoglycans　such　as　anti-inflammatory,　antiosteoperotic,　and　anti-asthmatic.　Glycosaminoglycans　are　complex　linear　polysaccharides　ubiquitous　in　mammals　that　possess　a　wide　range　of　biological　activities.　However,　their　presence　and　possible　structural　characteristics　within　O.ran.　were　previously　unknown.　In　this　study,　glycosaminoglycans　were　isolated　from　O.ran.　and　their　disaccharide　compositions　were　analyzed　by　liquid　chromatography-ion　trap/time-of-flight　mass　spectrometry　(LC-MS-ITTOF).　Heparan　sulfate　(HS)/heparin　(HP),　chondroitin　sulfate　(CS)/dermatan　sulfate　(DS)　and　hyaluronic　acid　(HA)　were　detected　in　O.ran.　with　varied　disaccharide　compositions.　HS　species　contain　highly　acetylated　disaccharides,　and　have　various　structures　in　their　constituent　chains.　CS/DS　chains　also　possess　a　heterogeneous　structure　with　different　sulfation　patterns　and　densities.　This　novel　structural　information　could　help　clarify　the　possible　involvement　of　these　polysaccharides　in　the　biological　activities　of　O.ran..