Colla　corii　asini　(CCA)　made　from　donkey-hide　has　been　widely　used　as　a　traditional　animal-based　Chinese　medicine.　Chondroitin　sulfate　(CS),　dermatan　sulfate　(DS)　and　hyaluronic　acid　(HA)　are　structurally　complex　classes　of　glycosaminoglycans　(GAGs)　that　have　been　implicated　in　a　wide　range　of　biological　activities.　However,　their　possible　structural　characteristics　in　CCA　are　not　clear.　In　this　study,　GAG　fractions　containing　CS/DS　and　HA　were　isolated　from　CCA　and　their　disaccharide　compositions　were　analyzed　by　high　sensitivity　liquid　chromatography-ion　trap/time-of-flight　mass　spectrometry　(LC-MS-ITTOF).　The　result　showed　that　CS/DS/HA　disaccharides　were　detected　in　the　three　lower　salt　fractions　from　anion-exchange　chromatography.　The　sulfation　patterns　and　densities　of　CS/DS　chains　in　these　fractions　differed　greatly,　while　HA　chains　varied　in　their　chain　lengths.　The　quantitative　analysis　first　revealed　that　the　amount　of　GAGs　in　CCA　varied　significantly　in　total　and　in　each　fraction.　This　novel　structural　information　could　help　clarify　the　possible　involvement　of　these　polysaccharides　in　the　biological　activities　of　CCA.　©　2020,　Springer　Science+Business　Media,　LLC,　part　of　Springer　Nature.