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author:

Wu, Ai-Hong (Wu, Ai-Hong.) [1] | Sun, Jian-Jun (Sun, Jian-Jun.) [2] (Scholars:孙建军) | Su, Xiu-Li (Su, Xiu-Li.) [3] | Lin, You-Wen (Lin, You-Wen.) [4] | Lin, Zhi-Bin (Lin, Zhi-Bin.) [5] | Yang, Huang-Hao (Yang, Huang-Hao.) [6] (Scholars:杨黄浩) | Chen, Guo-Nan (Chen, Guo-Nan.) [7] (Scholars:陈国南)

Indexed by:

Scopus SCIE

Abstract:

Cathodic electrochemiluminescence (ECL) is firstly observed at a carbon oxide covered glassy carbon (C/CxO1-x) electrode as a large cathodic pulse polarization is applied. This insulating carbon oxide (CxO1-x) film is constructed on a glassy carbon (GC) substrate by electrochemical oxidization in basic media. The film properties, such as the composition of carbon and oxygen, and the thickness as well, can be controlled by the potential and the duration in the oxidizing process. X-Ray photoelectron spectroscopy (XPS) studies show that carbonyl and carboxyl dominate at the oxidized surface, to which antibodies can be covalently bound. The specific immunoreaction between antigen (Ag) and antibody (Ab) resulted in a decrease in the ECL intensity, thus creating an interesting basis for the development of a label-free cathodic ECL immunosensor. As an example, human IgG (hIgG) was sensitively determined in the concentration range of 0.01-100 ng mL(-1), and the detection limit was ca. 1.0 pg mL(-1) (S/N 3). In addition, the content of hIgG in human serum has been assayed by the developed immunosensor and a commercially available immune turbidimetry method, respectively, and consistent results were obtained. The prepared immunosensor provides a promising approach for the clinical determination of IgG levels in human serum, because it is simple, rapid, highly sensitive, specific, and without the need of tedious labeling operations.

Keyword:

Community:

  • [ 1 ] [Wu, Ai-Hong]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 2 ] [Sun, Jian-Jun]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 3 ] [Su, Xiu-Li]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 4 ] [Lin, You-Wen]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 5 ] [Lin, Zhi-Bin]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 6 ] [Yang, Huang-Hao]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 7 ] [Chen, Guo-Nan]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
  • [ 8 ] [Wu, Ai-Hong]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 9 ] [Sun, Jian-Jun]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 10 ] [Su, Xiu-Li]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 11 ] [Lin, You-Wen]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 12 ] [Lin, Zhi-Bin]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 13 ] [Yang, Huang-Hao]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 14 ] [Chen, Guo-Nan]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Dept Chem, Fuzhou 350108, Peoples R China
  • [ 15 ] [Lin, You-Wen]Fujian Med Univ, Dept Pharmaceut Anal, Fac Pharm, Fuzhou 350004, Peoples R China

Reprint 's Address:

  • 孙建军

    [Sun, Jian-Jun]Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China

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Source :

ANALYST

ISSN: 0003-2654

Year: 2010

Issue: 9

Volume: 135

Page: 2309-2315

3 . 9 1 3

JCR@2010

3 . 6 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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