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Abstract ：
The flap endonuclease 1 (FEN1) plays a key role in DNA replication and repair, its aberrant expression is associated with tumor development, so it has been recognized as a promising biomarker for a variety of cancers. Here, a novel "turn on" mode gold nanocube-enhanced surface-enhanced Raman scattering (SERS) biosensor was constructed by combining a heated Au electrode (HAuE), exonuclease III (Exo III)-assisted cycle amplification, and gold nanocube (AuNC)-based SERS enhancement to achieve highly sensitive detection of FEN1 activity. The SERS tag was prepared using the Raman reporter modified on the AuNC surface, and the high electromagnetic field provided by the sharp geometric feature of AuNC greatly enhanced the SERS signal. At the same time, HAuE was used to increase the electrode surface temperature and enhance the FEN1 activity, leading to more trigger DNA being cleaved, which was used to initiate the Exo III-assisted cycle amplification. Taking all these advantages, the proposed method possessed high sensitivity and good selectivity, with a low limit of detection (LOD) of 3.19 x 10- 7 U mu L- 1. In addition, this method was successfully applied to detect FEN1 activity in real cellular extracts.
Keyword ：
Exo III-Assisted cycle amplification Exo III-Assisted cycle amplification Flap endonuclease 1 (FEN1) Flap endonuclease 1 (FEN1) Heated Au electrode Heated Au electrode Surface enhanced Raman spectroscopy (SERS) Surface enhanced Raman spectroscopy (SERS)
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| GB/T 7714 | Jin, Lei-Xin , Wang, Qi-Meng , Zhu, Shu-Jiao et al. Gold nanocube-enhanced SERS biosensor based on heated electrode coupled with exonuclease III-assisted cycle amplification for sensitive detection of flap endonuclease 1 activity [J]. | TALANTA , 2025 , 286 . |
| MLA | Jin, Lei-Xin et al. "Gold nanocube-enhanced SERS biosensor based on heated electrode coupled with exonuclease III-assisted cycle amplification for sensitive detection of flap endonuclease 1 activity" . | TALANTA 286 (2025) . |
| APA | Jin, Lei-Xin , Wang, Qi-Meng , Zhu, Shu-Jiao , Zhu, Cai-Lian , Sun, Jian-Jun , Wu, Shao-Hua . Gold nanocube-enhanced SERS biosensor based on heated electrode coupled with exonuclease III-assisted cycle amplification for sensitive detection of flap endonuclease 1 activity . | TALANTA , 2025 , 286 . |
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2,2-methylenebis(4-chlorophenol) also known as dichlorophen (Dcp) is a highly toxic environmental pollutant that persists at low concentrations and is notoriously resistant to degradation. Consequently, it is highly important to develop a simple and rapid detection assay for Dcp. This study presents a one-pot synthesis of novel nanocomposites based on a lanthanum-based metal-organic framework (La-MOF), water-soluble R-cyclodextrin (R-CD) and fullerene (C60). La-MOF@C60/R-CD nanocomposite was integrated into a glassy carbon electrode (GCE) to create a new electrochemical sensor for Dcp detection. La-MOFs have a large specific surface area with copious electrocatalytic active sites, but the low conductivity of La-MOF limits its electrochemical performance. To overcome this, the conductivity was improved by the incorporation of C60 into La-MOF. Furthermore, the host-guest interaction facilitated by R-CD endows the La-MOF@C60/R-CD-modified electrode with superior electrocatalytic activity towards Dcp oxidation. The resulting sensor offers sensitive Dcp detection, with a linear response range from 30 nM to 25 mu M and a limit of detection (LOD) of 11.6 nM. It also exhibits excellent signal reproducibility, sensor stability, and selectivity towards Dcp. Furthermore, the strategy was effectively applied to the determination of Dcp in real water samples, achieving satisfactory recoveries between 93.3 % and 102.6 %.
Keyword ：
2,2-Methylenebis(4-chlorophenol) 2,2-Methylenebis(4-chlorophenol) C 60 C 60 Electrochemical detection Electrochemical detection La-MOF La-MOF R-CD R-CD
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| GB/T 7714 | Xie, Yu , Zhou, Wei , Yin, Jia-Wen et al. A novel electrochemical sensor based on La-MOF@C60-R-cyclodextrin composite for sensitive detection of dichlorophen in lake and tap water [J]. | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2025 , 13 (1) . |
| MLA | Xie, Yu et al. "A novel electrochemical sensor based on La-MOF@C60-R-cyclodextrin composite for sensitive detection of dichlorophen in lake and tap water" . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 13 . 1 (2025) . |
| APA | Xie, Yu , Zhou, Wei , Yin, Jia-Wen , Li, Yi , Hou, Lin-Li , Liu, Li-Min et al. A novel electrochemical sensor based on La-MOF@C60-R-cyclodextrin composite for sensitive detection of dichlorophen in lake and tap water . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2025 , 13 (1) . |
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Background: The fabrication of sensors capable of achieving rapid, sensitive, and highly selective detection of target molecules in complex fluids is key to realizing their real-world applications. For example, there is an urgent need in drugged driving roadside screening scenarios to develop a method that can be used for rapid drug detection and that avoids interference from the matrix in the sample. How to minimize the interference of complex matrices in biofluids at the electrode interface is the key to improve the sensitivity of the sensor. Results: This work develops a facile and green method to prepare rough electrodes with a porous structure for constructing electrochemical aptamer-based (EAB) sensors for rapid, sensitive and accurate detection of Delta(9 )tetrahydrocannabinol (THC) in biofluids. The electroactive area of the rough electrode was 21 times of smooth electrode. And the antifouling performance of the rough electrode was much better than that of smooth electrode. Based on the unique advantages of the rough electrode, the developed EAB sensor achieves rapid nanomolar detection of THC in undiluted serum, undiluted urine and 50 % saliva with the detection limit of 5.0 nM, 10 nM and 10 nM, respectively. Moreover, our method possesses good reproducibility, accuracy and specificity. Significance: The porous structure can effectively reduce the non-specific adsorption and enhance the stability of the signal, while the larger active area can modify more aptamers, thus improving the sensitivity. The detection limits of the EAB sensor were lower than the cutoff concentration of THC in drugged driving and the measuring process was completed within 60 s after target addition, which makes the present sensors capable for real-world applications.
Keyword ：
Biofluids Biofluids Delta(9)-tetrahydrocannabinol Delta(9)-tetrahydrocannabinol Drugged driving screening Drugged driving screening EAB sensor EAB sensor Nanomolar detection Nanomolar detection Rough electrode Rough electrode
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| GB/T 7714 | Xie, Yu , She, Jin-Ping , Zheng, Jia-Xing et al. Rapid nanomolar detection of Δ9-tetrahydrocannabinol in biofluids via electrochemical aptamer-based biosensor [J]. | ANALYTICA CHIMICA ACTA , 2024 , 1295 . |
| MLA | Xie, Yu et al. "Rapid nanomolar detection of Δ9-tetrahydrocannabinol in biofluids via electrochemical aptamer-based biosensor" . | ANALYTICA CHIMICA ACTA 1295 (2024) . |
| APA | Xie, Yu , She, Jin-Ping , Zheng, Jia-Xing , Salminen, Kalle , Sun, Jian-Jun . Rapid nanomolar detection of Δ9-tetrahydrocannabinol in biofluids via electrochemical aptamer-based biosensor . | ANALYTICA CHIMICA ACTA , 2024 , 1295 . |
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Cocaine is one of the most abused illicit drugs, and its abuse damages the central nervous system and can even lead directly to death. Therefore, the development of simple, rapid and highly sensitive detection methods is crucial for the prevention and control of drug abuse, traffic accidents and crime. In this work, an electrochemical aptamer-based (EAB) sensor based on the low-temperature enhancement effect was developed for the direct determination of cocaine in bio-samples. The signal gain of the sensor at 10 degrees C was greatly improved compared to room temperature, owing to the improved affinity between the aptamer and the target. Additionally, the electroactive area of the gold electrode used to fabricate the EAB sensor was increased 20 times by a simple electrochemical roughening method. The porous electrode possesses more efficient electron transfer and better antifouling properties after roughening. These improvements enabled the sensor to achieve rapid detection of cocaine in complex bio-samples. The low detection limits (LOD) of cocaine in undiluted urine, 50% serum and 50% saliva were 70 nM, 30 nM and 10 nM, respectively, which are below the concentration threshold in drugged driving screening. The aptasensor was simple to construct and reusable, which offers potential for drugged driving screening in the real world.
Keyword ：
Biofluids Biofluids Cocaine Cocaine Drugged driving screening Drugged driving screening EAB sensor EAB sensor Low-temperature enhancement effect Low-temperature enhancement effect Porous gold electrode Porous gold electrode Square wave voltammetry Square wave voltammetry
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| GB/T 7714 | Xie, Yu , Huang, Da-Dong , Xu, Ling-Feng et al. Rapid nanomolar detection of cocaine in biofluids by electrochemical aptamer-based sensor with low-temperature effect for drugged driving screening [J]. | MICROCHIMICA ACTA , 2024 , 191 (9) . |
| MLA | Xie, Yu et al. "Rapid nanomolar detection of cocaine in biofluids by electrochemical aptamer-based sensor with low-temperature effect for drugged driving screening" . | MICROCHIMICA ACTA 191 . 9 (2024) . |
| APA | Xie, Yu , Huang, Da-Dong , Xu, Ling-Feng , Wan, Ting , Cao, Yi-Jie , Salminen, Kalle et al. Rapid nanomolar detection of cocaine in biofluids by electrochemical aptamer-based sensor with low-temperature effect for drugged driving screening . | MICROCHIMICA ACTA , 2024 , 191 (9) . |
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The metabolic differences between patients with the anticancer drug daunorubicin (DRN) pose difficulties in providing clinical delivery strategies for different patients. Therefore, it is urgent to develop a pharmacokinetic platform that can real-time monitor drug levels in vivo to achieve precise drug delivery and reduce drug side effects. To this end, an electrochemical aptamer-based (E-AB) sensor was designed to realize real-time and continuous monitoring of DRN levels in vivo and obtain DRN pharmacokinetics of different individuals for the first. Specifically, the binding free energy of the aptamer to the target was calculated by molecular docking simulations. Using nanoporous gold working electrodes with immobilized aptamers as E-AB sensors, which monitored DRN in the blood of different rat models continuously for many hours with nanomolar precision and second resolution, and hundreds of DRN concentration values were obtained, based on which important pharmacokinetic parameters were simulated and computed, including half-life of drug DRN distribution and elimination, and peak concentration in blood. These parameters help to determine the metabolism of drug DRN in different patients and adjust the administered dose in time to achieve precise treatment. Therefore, this E-AB platform may provide a valuable new tool for clinicians to monitor pharmacokinetics in different patients.
Keyword ：
Aptamer Aptamer Daunorubicin Daunorubicin Drug monitoring Drug monitoring Electrochemical sensor Electrochemical sensor In vivo In vivo Nanoporous gold Nanoporous gold
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| GB/T 7714 | Qin, Sai-Nan , Jie, Zhi-Qiang , Chen, Li -Yang et al. Real-time monitoring of daunorubicin pharmacokinetics with nanoporous electrochemical aptamer-based sensors in vivo [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 411 . |
| MLA | Qin, Sai-Nan et al. "Real-time monitoring of daunorubicin pharmacokinetics with nanoporous electrochemical aptamer-based sensors in vivo" . | SENSORS AND ACTUATORS B-CHEMICAL 411 (2024) . |
| APA | Qin, Sai-Nan , Jie, Zhi-Qiang , Chen, Li -Yang , Zheng, Jia-Xing , Xie, Yu , Feng, Lei et al. Real-time monitoring of daunorubicin pharmacokinetics with nanoporous electrochemical aptamer-based sensors in vivo . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 411 . |
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Imatinib (Ima), as a commonly used anticancer drug for the clinical treatment of leukemia and gastrointestinal mesenchymal stromal tumour, requires timely monitoring of patients' blood concentration to ensure efficacy while reducing complications and achieving precision medicine due to its narrow therapeutic window (1-5 mu M) and the varying sensitivity and resistance of different patients to Ima. However, traditional assays are slow and cumbersome, so improved and innovative platforms for monitoring Ima in the clinic are necessary. In this work, a nanoporous electrochemical aptamer-based (E-AB) sensor was designed for the detection of Ima and imatinib mesylate (Ima-Mes) in blood. Apt-37, a high-affinity and conformationally variable aptamer, was screened by molecular docking simulation calculations and circular dichroism (CD) for the construction of the E-AB sensor. The sensor detected Ima and Ima-Mes in the range of 0.1 mu M-1 mM, and the recoveries in spiked blood samples were in the range of 70.7 %-104.6 % and 74.8 %-113.9 %, respectively. The precision and accuracy of the E-AB sensor for measuring Ima-Mes concentration in blood was similar to the standard LC-MS method. These results demonstrate that the developed E-AB sensor is an effective tool for rapid monitoring of Ima and Ima-Mes in blood.
Keyword ：
Aptamer Aptamer Blood concentration Blood concentration Electrochemical sensors Electrochemical sensors Imatinib detection Imatinib detection
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| GB/T 7714 | Qin, Sai-Nan , Xie, Hai-Hong , Cao, Yi-Jie et al. Construction of an electrochemical aptamer-based sensors for rapid quantification of the anticancer drug imatinib in blood to improve drug bioavailability at microdoses [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 282 . |
| MLA | Qin, Sai-Nan et al. "Construction of an electrochemical aptamer-based sensors for rapid quantification of the anticancer drug imatinib in blood to improve drug bioavailability at microdoses" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 282 (2024) . |
| APA | Qin, Sai-Nan , Xie, Hai-Hong , Cao, Yi-Jie , Wan, Ting , Feng, Lei , Salminen, Kalle et al. Construction of an electrochemical aptamer-based sensors for rapid quantification of the anticancer drug imatinib in blood to improve drug bioavailability at microdoses . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 282 . |
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Real-time, high-frequency measurements of pharmaceuticals, metabolites, exogenous antigens, and other biomolecules in biological samples can provide critical information for health management and clinical diagnosis. Electrochemical aptamer-based (EAB) sensor is a promising analytical technique capable of achieving these goals. However, the issues of insufficient sensitivity, frequent calibration and lack of adapted portable electrochemical device limit its practical application in immediate detection. In response we have fabricated an on-chip-integrated, cold-hot Janus EAB (J-EAB) sensor based on the thermoelectric coolers (TECs). Attributed to the Peltier effect, the enhanced/suppressed current response can be generated simultaneously on cold/hot sides of the J-EAB sensor. The ratio of the current responses on the cold and hot sides was used as the detection signal, enabling rapid on-site, calibration-free determination of small molecules (procaine) as well as macromolecules (SARS-CoV-2 spike protein) in single step, with detection limits of 1 mu M and 10 nM, respectively. We have further demonstrated that the J-EAB sensor is effective in improving the ease and usability of the actual detection process, and is expected to provide a universal, low-cost, fast and easy potential analytical tool for other clinically important biomarkers, drugs or pharmaceutical small molecules.
Keyword ：
Calibration-free Calibration-free Cold-hot Cold-hot Electrochemical aptamer-based sensor Electrochemical aptamer-based sensor Janus Janus Peltier effect Peltier effect
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| GB/T 7714 | Feng, Lei , Gao, Run-Yu , Chen, Zhi-Min et al. Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules [J]. | BIOSENSORS & BIOELECTRONICS , 2024 , 264 . |
| MLA | Feng, Lei et al. "Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules" . | BIOSENSORS & BIOELECTRONICS 264 (2024) . |
| APA | Feng, Lei , Gao, Run-Yu , Chen, Zhi-Min , Qin, Sai-Nan , Cao, Yi-Jie , Salminen, Kalle et al. Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules . | BIOSENSORS & BIOELECTRONICS , 2024 , 264 . |
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Electrodeposition plays an indispensable role in microelectronic process. Copper pillar bumps (CPBs) have received widespread attention in advanced packaging. Clarifying the effects of additives is essential to obtain the uniform, leveling and void-free filled layer. However, the observation platform to achieve efficient screening of various additive combinations is absent. Herein, taking advantage of the highly transparent characteristic of microcavity electrode (MCE) as well as its similarity to the practical working conditions of CPBs, the convection-dependence of the copper filling layer (500-25 mu m, aspect ratio similar to 1) was observed. The leveling effect of the additives with the modulating flow and electric fields was visualized. In-situ observations and electrochemical evidences support that accelerated growth of the downstream front can only be observed when all three organic additives are present simultaneously. Although the introduction of convection can accelerate the renewal of additives on the surface of the filled layer, the antagonistic effect of Janus Green B (JGB) on sodium 3,3 '-disulfanediylbis(propane-1-sulfonate) (SPS) is weakened due to the rapid decomposition of JGB within the microcavity, thereby amplifying the accumulation effect of SPS downstream in the microcavity. Consequently, the competitiveness of SPS against polyethylene glycol (PEG) on that side (depolarization) is enhanced, and a non-uniform distribution of the filled layer is established. As the protrusion approaches the mouth of the microcavity, the sufficient supply of JGB reverses the height bias. This work demonstrates the value of this in-situ observation platform in parameters optimizing and mechanisms studying.
Keyword ：
Convection Convection Copper filling Copper filling Copper pillar bump Copper pillar bump In-situ observation In-situ observation Janus green B Janus green B
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| GB/T 7714 | Feng, Lei , Chen, Shu-Ning , Huang, Da -Dong et al. In-situ observation and electrochemical studies on copper pillar bump filling process [J]. | JOURNAL OF ELECTROANALYTICAL CHEMISTRY , 2024 , 967 . |
| MLA | Feng, Lei et al. "In-situ observation and electrochemical studies on copper pillar bump filling process" . | JOURNAL OF ELECTROANALYTICAL CHEMISTRY 967 (2024) . |
| APA | Feng, Lei , Chen, Shu-Ning , Huang, Da -Dong , Li, Guang-Wei , Zheng, Jia-Xing , Salminen, Kalle et al. In-situ observation and electrochemical studies on copper pillar bump filling process . | JOURNAL OF ELECTROANALYTICAL CHEMISTRY , 2024 , 967 . |
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Hydrated electrons were generated at boron doped diamond electrodes in fully aqueous solutions during cathodic pulse polarization and utilized for the electrochemiluminescence (ECL) determination of alkaline phosphatase (ALP) by adapting one of the most common fluorescent methods. In this approach, highly fluorescent 4-methylumbelliferyl (MU) is enzymatically generated from non-fluorescent 4-methylumbelliferyl phosphate (MUP) substrate and chemically excited in the presence of oxidizing radicals and reducing hydrated electrons. A novel time-resolved internal standard strategy is presented to counter matrix effects to certain degree and to lower signal variation among concurrent measurements. In this unique approach, the cathodic ECL signals of MU and Tb(III)-chelate were separated on the basis of their exceptionally different excited state lifetimes. This enabled more precise determination of ALP by simply measuring the ratio of ECL emissions during and after cathodic excitation pulse with simple two-electrode, single photomultiplier tube setup. The ratiometric ECL signals in the absence and presence of 1.0 % serum (v/v) were interchangeable whereas ratiometric signal recovery in the presence of 10.0 % serum was similar to 85 %.
Keyword ：
Alkaline phosphatase Alkaline phosphatase Boron doped diamond electrodes Boron doped diamond electrodes Electrochemiluminescence Electrochemiluminescence Hydrated electrons Hydrated electrons Ratiometric measurement Ratiometric measurement
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| GB/T 7714 | Salminen, Kalle , Chen, Li -Yang , Irkham et al. Simple hydrated electron-induced electrochemiluminescence determination of alkaline phosphatase with novel time-resolved internal standard strategy at boron doped diamond electrodes [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 417 . |
| MLA | Salminen, Kalle et al. "Simple hydrated electron-induced electrochemiluminescence determination of alkaline phosphatase with novel time-resolved internal standard strategy at boron doped diamond electrodes" . | SENSORS AND ACTUATORS B-CHEMICAL 417 (2024) . |
| APA | Salminen, Kalle , Chen, Li -Yang , Irkham , Kulmala, Sakari , Einaga, Yasuaki , Sun, Jian-Jun . Simple hydrated electron-induced electrochemiluminescence determination of alkaline phosphatase with novel time-resolved internal standard strategy at boron doped diamond electrodes . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 417 . |
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Acute myocardial infarction (AMI) is one of the top contributors to global disease mortality. AMI biomarkers, such as cardiac troponin I (cTnI), are often detected with enzyme-linked immunosorbent assay (ELISA) that suffers from several well-known drawbacks such as poor stability and slow and cumbersome operation. Therefore, it is necessary to develop a new analytical technique that can rapidly analyse and detect cTnI for early screening of AMI. In this work, a nanoporous electrochemical aptamer-based (E-AB) sensor for rapid and sensitivite detect of cTnI was designed. Firstly, the aptamer was truncated, and then molecular docking simulation and circular dichroism (CD) were used to screen for aptamers with significant conformational changes when binding to the target, in order to enhance the sensitivity of E-AB sensors. Subsequently, nanoporous electrodes with active area 20 times higher than that of smooth electrodes were fabricated by electrochemical alloying/ dealloying, which enabled E-AB sensors to obtain higher signal-to-noise ratios, providing favorable assurance for the detection results. Under optimal conditions, E-AB sensors could specifically detect cTnI in serum and blood with a detection limit of 1 pg/mL. At the same time, the sensor and enzyme-linked immunoassay (ELISA) had identical detection results when measuring target levels from real clinical samples. Furthermore, the sensor exhibited good reproducibility, stability, providing a simple and low-cost method for detecting cTnI, which is expected to help early AMI patients obtain accurate diagnosis.
Keyword ：
Acute myocardial infarction Acute myocardial infarction Cardiac troponin I Cardiac troponin I E -AB sensor E -AB sensor High sensitivity High sensitivity Nanoporous electrodes Nanoporous electrodes Rapid detection Rapid detection
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| GB/T 7714 | Qin, Sai-Nan , Nong, Yun-Chuan , Cao, Chuan-Liang et al. A nanoporous electrochemical aptamer-based sensors for rapid detection of cardiac troponin I in blood [J]. | TALANTA , 2024 , 284 . |
| MLA | Qin, Sai-Nan et al. "A nanoporous electrochemical aptamer-based sensors for rapid detection of cardiac troponin I in blood" . | TALANTA 284 (2024) . |
| APA | Qin, Sai-Nan , Nong, Yun-Chuan , Cao, Chuan-Liang , Chen, Li-Yang , Cao, Yi-Jie , Wan, Ting et al. A nanoporous electrochemical aptamer-based sensors for rapid detection of cardiac troponin I in blood . | TALANTA , 2024 , 284 . |
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