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author:

Wei, Xiaofeng (Wei, Xiaofeng.) [1] | Zheng, Lingyan (Zheng, Lingyan.) [2] | Luo, Fang (Luo, Fang.) [3] (Scholars:罗芳) | Lin, Zhenyu (Lin, Zhenyu.) [4] (Scholars:林振宇) | Guo, Longhua (Guo, Longhua.) [5] | Qiu, Bin (Qiu, Bin.) [6] (Scholars:邱彬) | Chen, Guonan (Chen, Guonan.) [7] (Scholars:陈国南)

Indexed by:

EI Scopus SCIE

Abstract:

Based on the adsorption of a metal-organic framework (MOF) to DNA, a fluorescent biosensor for H5N1 antibodies has been developed. In this system, a short oligonucleotide was modified with the fluorescent dye 5'6-FAM (5'6-carboxyfluorescein) as the fluorescent DNA probe. With the introduction of a MOF aqueous solution, the DNA probe can be adsorbed by the MOF and the fluorescence of the dye will be quenched by the MOF. Subsequently, exonuclease I (Exo I) is employed to specifically hydrolyze the DNA probe at the 3'-terminus and the fluorescent dye FAM is released from the MOF, which results in recovery of the fluorescence. If the 3'-ends of the DNA probe are linked to H5N1 antigens, which can specifically recognize the H5N1 antibody, the hydrolysis of Exo I would be inhibited, so the fluorescence of the system would not recover. The fluorescence is related to the logarithm of the H5N1 antibody concentration in the range 1.0 x 10(-6)-5.0 x 10(-9) mol L-1 with a detection limit of 1.6 x 10(-9) mol L-1 (S/N = 3). The proposed method has been applied to detect the H5N1 antibody in serum samples with satisfactory results.

Keyword:

Community:

  • [ 1 ] [Wei, Xiaofeng]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
  • [ 2 ] [Zheng, Lingyan]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
  • [ 3 ] [Luo, Fang]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
  • [ 4 ] [Lin, Zhenyu]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
  • [ 5 ] [Guo, Longhua]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
  • [ 6 ] [Qiu, Bin]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China
  • [ 7 ] [Chen, Guonan]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China

Reprint 's Address:

  • 魏晓峰

    [Wei, Xiaofeng]Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, Fujian Prov Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350002, Peoples R China

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Source :

JOURNAL OF MATERIALS CHEMISTRY B

ISSN: 2050-750X

Year: 2013

Issue: 13

Volume: 1

Page: 1812-1817

6 . 1 0 0

JCR@2023

ESI Discipline: MATERIALS SCIENCE;

JCR Journal Grade:4

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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