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author:

Wei, Xiaofeng (Wei, Xiaofeng.) [1] | Zheng, Lingyan (Zheng, Lingyan.) [2] | Luo, Fang (Luo, Fang.) [3] | Lin, Zhenyu (Lin, Zhenyu.) [4] | Guo, Longhua (Guo, Longhua.) [5] | Qiu, Bin (Qiu, Bin.) [6] | Chen, Guonan (Chen, Guonan.) [7]

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EI

Abstract:

Based on the adsorption of a metal-organic framework (MOF) to DNA, a fluorescent biosensor for H5N1 antibodies has been developed. In this system, a short oligonucleotide was modified with the fluorescent dye 5′6-FAM (5′6-carboxyfluorescein) as the fluorescent DNA probe. With the introduction of a MOF aqueous solution, the DNA probe can be adsorbed by the MOF and the fluorescence of the dye will be quenched by the MOF. Subsequently, exonuclease I (Exo I) is employed to specifically hydrolyze the DNA probe at the 3′-terminus and the fluorescent dye FAM is released from the MOF, which results in recovery of the fluorescence. If the 3′-ends of the DNA probe are linked to H5N1 antigens, which can specifically recognize the H5N1 antibody, the hydrolysis of Exo I would be inhibited, so the fluorescence of the system would not recover. The fluorescence is related to the logarithm of the H5N1 antibody concentration in the range 1.0 × 10-6-5.0 × 10-9 mol L-1 with a detection limit of 1.6 × 10-9 mol L-1 (S/N = 3). The proposed method has been applied to detect the H5N1 antibody in serum samples with satisfactory results. © The Royal Society of Chemistry 2013.

Keyword:

Antibodies Biosensors Chemical detection Crystalline materials DNA Fluorescence Hydrolysis Oligonucleotides Organometallics Probes

Community:

  • [ 1 ] [Wei, Xiaofeng]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 2 ] [Zheng, Lingyan]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 3 ] [Luo, Fang]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 4 ] [Lin, Zhenyu]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 5 ] [Guo, Longhua]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 6 ] [Qiu, Bin]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 7 ] [Chen, Guonan]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China

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Source :

Journal of Materials Chemistry B

ISSN: 2050-7518

Year: 2013

Issue: 13

Volume: 1

Page: 1812-1817

6 . 1 0 0

JCR@2023

JCR Journal Grade:4

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 94

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

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