• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Wei, X. (Wei, X..) [1] | Zheng, L. (Zheng, L..) [2] | Luo, F. (Luo, F..) [3] | Lin, Z. (Lin, Z..) [4] | Guo, L. (Guo, L..) [5] | Qiu, B. (Qiu, B..) [6] | Chen, G. (Chen, G..) [7]

Indexed by:

Scopus

Abstract:

Based on the adsorption of a metal-organic framework (MOF) to DNA, a fluorescent biosensor for H5N1 antibodies has been developed. In this system, a short oligonucleotide was modified with the fluorescent dye 5′6-FAM (5′6-carboxyfluorescein) as the fluorescent DNA probe. With the introduction of a MOF aqueous solution, the DNA probe can be adsorbed by the MOF and the fluorescence of the dye will be quenched by the MOF. Subsequently, exonuclease I (Exo I) is employed to specifically hydrolyze the DNA probe at the 3′-terminus and the fluorescent dye FAM is released from the MOF, which results in recovery of the fluorescence. If the 3′-ends of the DNA probe are linked to H5N1 antigens, which can specifically recognize the H5N1 antibody, the hydrolysis of Exo I would be inhibited, so the fluorescence of the system would not recover. The fluorescence is related to the logarithm of the H5N1 antibody concentration in the range 1.0 × 10-6-5.0 × 10-9 mol L-1 with a detection limit of 1.6 × 10-9 mol L-1 (S/N = 3). The proposed method has been applied to detect the H5N1 antibody in serum samples with satisfactory results. © The Royal Society of Chemistry 2013.

Keyword:

Community:

  • [ 1 ] [Wei, X.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 2 ] [Zheng, L.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 3 ] [Luo, F.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 4 ] [Lin, Z.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 5 ] [Guo, L.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 6 ] [Qiu, B.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China
  • [ 7 ] [Chen, G.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China

Reprint 's Address:

  • [Lin, Z.]Department of Chemistry, Fuzhou University, Ministry of Education, Fuzhou 350002, China

Email:

Show more details

Related Keywords:

Related Article:

Source :

Journal of Materials Chemistry B

ISSN: 2050-7518

Year: 2013

Issue: 13

Volume: 1

Page: 1812-1817

6 . 1 0 0

JCR@2023

JCR Journal Grade:4

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 94

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Affiliated Colleges:

Online/Total:177/10755301
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1