This　work　reports　a　new　electrochemical　monitoring　platform　for　sensitive　detection　of　Cu2+　coupling　click　chemistry　with　nanogold-functionalized　PAMAM　dendrimer　(AuNP-PAMAM).　The　system　involved　an　alkyne-modified　carbon　electrode　and　an　azide-functionalized　AuNP-PAMAM.　Initially,　the　added　Cu2+　was　reduced　to　Cu+　by　the　ascorbate,　and　then　the　azide-modified　AuNP-PAMAM　was　covalently　conjugated　to　the　electrode　via　Cu+-catalyzed　azide-alkyne　click　reaction.　The　carried　AuNPs　accompanying　PAMAM　dendrimer　could　be　directly　monitored　by　stripping　voltammetry　after　acidic　pretreatment.　By　introduction　of　high-loading　PAMAM　dendrimer　with　gold　nanoparticles,　as　low　as　2.8pM　Cu2+　(ppt)　could　be　detected,　which　was　125-fold　lower　than　that　of　gold　nanoparticle-based　labeling　strategy.　The　method　exhibited　high　specificity　toward　target　Cu2+　against　other　potentially　interfering　ions,　and　was　applicable　for　monitoring　Cu2+　in　drinking　water　with　satisfactory　results.