Persistent　luminescence　nanoparticles　(PLNPs)　have　great　potential　for　bioimaging　because　they　can　eliminate　the　tissue　autofluorescence　and　improve　the　signal-to-noise　ratio　significantly.　High-temperature　calcination　is　a　necessary　process　for　the　PLNPs　to　achieve　high　luminescence　intensity　and　long　afterglow　time.　However,　high-temperature　calcination　usually　results　in　uncontrollable　morphology　and　poor　homogeneity　of　PLNPs,　which　greatly　limit　their　applications.　Therefore,　there　is　still　a　high　demand　to　find　a　suitable　method　for　synthesizing　PLNPs　with　high　luminescence　intensity　and　long　afterglow　time　while　maintaining　their　monodispersed　morphology.　Herein,　we　report　a　facile　silica　template　method　to　synthesize　PLNPs　with　a　kiwifruit-like　structure　that　can　tolerate　high-temperature　calcination.　The　as-prepared　kiwifruit-like　SiO2@ZnGa2O4:Cr3+@SiO2　PLNPs　have　enhanced　near-infrared　persistent　luminescence,　uniform　morphology　and　size,　and　good　biocompatibility.　Moreover,　the　SiO2@ZnGa2O4:Cr3+@SiO2　PLNPs　can　be　repeatedly　activated　by　soft　X-rays　in　situ　and　emit　near-infrared　persistent　luminescence　with　long　decay　time,　holding　great　potential　for　deep-tissue　and　long-term　in　vivo　bioimaging.　We　believe　that　this　study　will　open　new　perspectives　for　synthesizing　high-performance　PLNPs　for　optical　imaging　and　diversified　applications.