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author:

Wu, Hui (Wu, Hui.) [1] | Zhang, Xianjun (Zhang, Xianjun.) [2] | Wu, Baoqiang (Wu, Baoqiang.) [3] | Qian, Cheng (Qian, Cheng.) [4] | Zhang, Fang (Zhang, Fang.) [5] | Wang, Liu (Wang, Liu.) [6] | Ye, Zunzhong (Ye, Zunzhong.) [7] | Wu, Jian (Wu, Jian.) [8]

Indexed by:

EI

Abstract:

Herein, we developed a rapid method for detection of genetically modified soybean (GTS 40-3-2) products using loop-mediated isothermal amplification (LAMP). A crude 5-minute extraction method was established for DNA extraction from soybeans and soybean products. LAMP reaction for CaMV35S promoter was optimized and the fastest threshold time (Tt) was 14 min with 4 mM magnesium ions at 63 °C. A portable instrument was designed to perform real-time LAMP in the field. As little as 0.1% GM soybean, specifically, or 0.5% GM ingredients in Chinese traditional tofu could be detected in 30 min from sampling to results. We used this method to further assess other five soybean products to determine whether they contained transgenic ingredients and compared the results with those obtained using PCR, which suggested the proposed method was applicable for rapid detection of genetically modified soybean in food products. © 2020 Elsevier Ltd

Keyword:

Extraction Food products Isotherms Metal ions Polymerase chain reaction

Community:

  • [ 1 ] [Wu, Hui]College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou; 310058, China
  • [ 2 ] [Zhang, Xianjun]Hangzhou An Yu Technologies Co.,Ltd., Hangzhou; 310021, China
  • [ 3 ] [Wu, Baoqiang]Hangzhou An Yu Technologies Co.,Ltd., Hangzhou; 310021, China
  • [ 4 ] [Qian, Cheng]College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou; 310058, China
  • [ 5 ] [Zhang, Fang]College of Biological Science and Engineering, Fuzhou University, Fuzhou; 350108, China
  • [ 6 ] [Wang, Liu]Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, State Key Laboratory for Quality and Safety of Agro-products, Hangzhou; 310021, China
  • [ 7 ] [Ye, Zunzhong]College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou; 310058, China
  • [ 8 ] [Wu, Jian]College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou; 310058, China

Reprint 's Address:

  • [wang, liu]institute of quality and standard for agro-products, zhejiang academy of agricultural sciences, state key laboratory for quality and safety of agro-products, hangzhou; 310021, china

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Related Keywords:

Source :

Food Chemistry

ISSN: 0308-8146

Year: 2020

Volume: 323

7 . 5 1 4

JCR@2020

8 . 5 0 0

JCR@2023

ESI HC Threshold:116

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 23

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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