The　serious　combination　of　abundant　electrons/holes　in　bulk　primarily　hinders　the　efficiency　in　the　photocatalytic　reaction.　It　is　crucial　to　control　the　spatial　charge　dynamics　through　delicately　designing　the　crystal　configuration　of　photocatalyst.　In　this　work,　a　modified　tungsten　trioxide　nanosheet　colloid　(M-WO3)　was　synthesized　by　an　ion　exchange　method.　Compared　to　pristine　WO3　(P-WO3),　the　crystal　lattice　vibration　frequency　of　M-WO3　increases　from　2.8　meV　to　4.3　meV,　which　effectively　prohibits　electron-phonon　coupling　and　powerfully　accelerates　the　separation　and　transfer　of　photoinduced　charge　carriers.　Irradiated　by　visible-light,　M-WO3　shows　much　higher　photocatalytic　bacterial　inactivation　performance　than　P-WO3.　In　addition,　this　regulation　method　increases　the　surface　charges　of　the　WO3　colloid　to　improve　its　stability,　which　endows　this　colloid　photocatalyst　with　broad　prospects　in　practical　photocatalytic　antibacterial　applications.　This　work　offers　guidance　to　construct　efficiently　separated　photoinduced　electron/hole　pairs　of　the　colloid　photocatalyst　by　designing　its　crystal　structure.