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author:

Pan, Wenhao (Pan, Wenhao.) [1] | Chen, Linhuan (Chen, Linhuan.) [2] | Zhu, Shidan (Zhu, Shidan.) [3] | Li, Dongyu (Li, Dongyu.) [4] | Shen, Zhifa (Shen, Zhifa.) [5] | Wu, Zai-Sheng (Wu, Zai-Sheng.) [6] (Scholars:吴再生)

Indexed by:

EI Scopus SCIE

Abstract:

While the intracellular imaging of miRNA biomarkers is of significant importance for the diagnosis and treatment of human cancers, DNA assembled nanoprobe has recently attracted considerable attention for imaging intracellular biomolecules. However, the complex construction process, intrinsic vulnerability to nuclease degradation and the limited signal transduction efficiency hamper its widespread application. In this contribution, based on persistent autonomous molecular motion of DNAzyme walker along a nano-substrate track, a DNA nanosphere probe (PNLD) is developed for the sensitive intracellular miR-21 imaging. Specifically, DNA nanosphere (called PN, single-molecule nano-track) is assembled from only one palindromic substrate, into which the locking strand-silenced DNAzymes (LD) are installed in a controlled manner. PNLD (made of PN and LD) can protect all DNA components against nuclease attack and maintain its structural integrity in serum solution over 24 h. Upon the activation by target miRNA, DNAzyme walker can move on the substrate scattered within PNLD (or on the surface) and between different PNLD objects and cleave many DNA substrates, generating an amplified signal. As a result, miR-21 can be detected down to 6.83 pM without the detectable interference from co-existing nontarget miRNAs. Moreover, PNLD system can accurately screen the different expression levels of miR-21 within the same type of cells and different types of cells, which is consistent with gold standard polymerase chain reaction (PCR) assay. Via changing the target recognition sequence, the PNLD system can be suitable for the intracellular imaging of miR-155, exhibiting the desirable universality. In addition, the DNAzyme walker-based PNLD system can be used to distinguish cancer cells from healthy cells, implying the potential application in cancer diagnosis and prognosis.

Keyword:

Cell imaging DNA walker Fluorescence miRNA detection Single-molecule track

Community:

  • [ 1 ] [Pan, Wenhao]Fuzhou Univ, Coll Chem, Canc Metastasis Alert & Prevent Ctr,Fujian Prov Ke, Natl & Local Joint Biomed Engn Res Ctr Photodynam, Fuzhou 350002, Peoples R China
  • [ 2 ] [Chen, Linhuan]Fuzhou Univ, Coll Chem, Canc Metastasis Alert & Prevent Ctr,Fujian Prov Ke, Natl & Local Joint Biomed Engn Res Ctr Photodynam, Fuzhou 350002, Peoples R China
  • [ 3 ] [Zhu, Shidan]Fuzhou Univ, Coll Chem, Canc Metastasis Alert & Prevent Ctr,Fujian Prov Ke, Natl & Local Joint Biomed Engn Res Ctr Photodynam, Fuzhou 350002, Peoples R China
  • [ 4 ] [Li, Dongyu]Fuzhou Univ, Coll Chem, Canc Metastasis Alert & Prevent Ctr,Fujian Prov Ke, Natl & Local Joint Biomed Engn Res Ctr Photodynam, Fuzhou 350002, Peoples R China
  • [ 5 ] [Wu, Zai-Sheng]Fuzhou Univ, Coll Chem, Canc Metastasis Alert & Prevent Ctr,Fujian Prov Ke, Natl & Local Joint Biomed Engn Res Ctr Photodynam, Fuzhou 350002, Peoples R China
  • [ 6 ] [Pan, Wenhao]Wenzhou Med Univ, Sch Lab Med & Life Sci, Key Lab Lab Med, Minist Educ China, Wenzhou 325035, Zhejiang, Peoples R China
  • [ 7 ] [Shen, Zhifa]Wenzhou Med Univ, Sch Lab Med & Life Sci, Key Lab Lab Med, Minist Educ China, Wenzhou 325035, Zhejiang, Peoples R China
  • [ 8 ] [Wu, Zai-Sheng]Wenzhou Med Univ, Sch Lab Med & Life Sci, Key Lab Lab Med, Minist Educ China, Wenzhou 325035, Zhejiang, Peoples R China
  • [ 9 ] [Pan, Wenhao]Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Med Genet, Wenzhou 325035, Peoples R China
  • [ 10 ] [Shen, Zhifa]Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Med Genet, Wenzhou 325035, Peoples R China
  • [ 11 ] [Wu, Zai-Sheng]Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Med Genet, Wenzhou 325035, Peoples R China

Reprint 's Address:

  • [Wu, Zai-Sheng]Fuzhou Univ, Coll Chem, Canc Metastasis Alert & Prevent Ctr,Fujian Prov Ke, Natl & Local Joint Biomed Engn Res Ctr Photodynam, Fuzhou 350002, Peoples R China;;

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Source :

TALANTA

ISSN: 0039-9140

Year: 2024

Volume: 280

5 . 6 0 0

JCR@2023

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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