Wound　infections　hinder　the　healing　process　and　potentially　result　in　life-threatening　complications,　which　urgently　require　rapid　and　timely　detection　and　treatment　pathogens　during　the　early　stages　of　infection.　Here,　an　intelligent　wound　dressing　was　developed　to　enable　in　situ　detection　and　elimination　of　pathogenic　bacteria　through　a　combination　of　point-of-care　testing　and　antibacterial　photodynamic　therapy　technology.　The　dressing　is　an　injectable　hydrogel　composed　of　carboxymethyl　chitosan　and　oxidized　sodium　alginate,　with　addition　of　4-methylumphulone　beta-D-glucoside　(MUG)　and　up-converted　nanoparticles　coated　with　titanium　dioxide　(UCNPs@TiO2).　The　presence　of　bacteria　can　be　visually　detected　by　monitoring　the　blue　fluorescence　of　4-methylumbellione,　generated　through　the　reaction　between　MUG　and　the　pathogen-associated　enzyme.　The　UCNPs@TiO2　photosensitizers　were　synthesized　and　demonstrated　high　antibacterial　activity　through　the　generation　of　reactive　oxygen　species　when　exposed　to　near-infrared　irradiation.　Meanwhile,　a　smartphone-based　portable　detection　system　equipped　with　a　self-developed　Android　app　was　constructed　for　in　situ　detection　of　pathogens　in　mere　seconds,　detecting　as　few　as　103　colony-forming　unit.　Additionally,　the　dressing　was　tested　in　a　rat　infected　wound　model　and　showed　good　antibacterial　activity　and　pro-healing　ability.　These　results　suggest　that　the　proposed　intelligent　wound　dressing　has　potential　for　use　in　the　diagnosis　and　management　of　wound　infections.　Statement　of　significance　An　intelligent　wound　dressing　has　been　prepared　for　simultaneous　in　situ　detection　and　elimination　of　pathogenic　bacteria.　The　presence　of　bacteria　can　be　visually　detected　by　tracking　the　blue　fluorescence　of　the　dressing.　Moreover,　a　smartphone-based　detection　system　was　constructed　to　detect　and　diagnose　pathogenic　bacteria　before　reaching　the　infection　limit.　Meanwhile,　the　dressing　was　able　to　effectively　eliminate　key　pathogenic　bacteria　on　demand　through　antibacterial　photodynamic　therapy　under　NIR　irradiation.　The　proposed　intelligent　wound　dressing　enables　timely　detection　and　treatment　of　infectious　pathogens　at　an　early　stage,　which　is　beneficial　for　wound　management.　(c)　2023　Acta　Materialia　Inc.　Published　by　Elsevier　Ltd.　All　rights　reserved.