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学者姓名：石贤爱
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Abstract ：
Resveratrol (Res) is a natural polyphenol exhibiting anti-oxidant and anti-inflammatory activity. However, the applications of Res have been limited due to its low stability and water solubility. To enhance the bioaccessibility of Res, unfolding bovine serum albumin-modified selenium nanoparticles (UBSA@SeNPs) encapsulated within chitosan (CS)-coated Pickering emulsions (CS-UBSA@SeNPs-PE) were used to load Res. The results showed that Res-loaded CS(0.06 %)-UBSA@SeNPs-PE has small droplet size (16.13 μm), high gel properties and excellent antioxidant properties. During the simulated digestion process, CS reduced the release rate of Res from Res-loaded CS(0.06 %)-UBSA@SeNPs-PE (42.27 %) to reach a slow release effect. Importantly, Res could quickly release from CS-UBSA@SeNPs-PE within intestinal fluid or in the presence of chitosanase. In simulated absorption experiments, the intestinal permeability of Res in Res-loaded CS(0.06 %)-UBSA@SeNPs-PE were enhanced by 292.31 % compare to Res-loaded CS(0 %)-UBSA@SeNPs-PE. In pharmacokinetic studies, Res-loaded CS(0.06 %)-UBSA@SeNPs-PE had an area under the curve (AUC) up to 3467.99 ± 127.43 ng*h/mL. Furthermore, CS also improved the mucoadhesive nature of UBSA@SeNPs-PE, resulting in a gut-retention time of Res-loaded CS(0.06 %)-UBSA@SeNPs-PE that reached up to 60 h. In conclusion, CS-UBSA@SeNPs-PE can serve as an effective oral delivery system for improving the bioaccessibility of Res. © 2024 Elsevier B.V.
Keyword ：
Body fluids Body fluids Chitosan Chitosan Crosslinking Crosslinking Gels Gels Silicones Silicones Surface plasmon resonance Surface plasmon resonance
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| GB/T 7714 | Mei, Jingtao , Shi, Xianai , Chen, Mingmao et al. Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility [J]. | International Journal of Biological Macromolecules , 2025 , 289 . |
| MLA | Mei, Jingtao et al. "Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility" . | International Journal of Biological Macromolecules 289 (2025) . |
| APA | Mei, Jingtao , Shi, Xianai , Chen, Mingmao , Cui, Yanan , Fang, Chaoping , Yang, Licong . Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility . | International Journal of Biological Macromolecules , 2025 , 289 . |
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| GB/T 7714 | Yang, Jianmin , Cai, Fengying , Lv, Yicheng et al. Chitosan nonwoven fabric composited calcium alginate and adenosine diphosphate as a hemostatic bandage for acute bleeding wounds (vol 257, 128561, 2024) [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 297 . |
| MLA | Yang, Jianmin et al. "Chitosan nonwoven fabric composited calcium alginate and adenosine diphosphate as a hemostatic bandage for acute bleeding wounds (vol 257, 128561, 2024)" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 297 (2025) . |
| APA | Yang, Jianmin , Cai, Fengying , Lv, Yicheng , Jiang, Ting , Zhao, Xingkai , Hu, Xueli et al. Chitosan nonwoven fabric composited calcium alginate and adenosine diphosphate as a hemostatic bandage for acute bleeding wounds (vol 257, 128561, 2024) . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 297 . |
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The inadequate vascularization and abnormal immune microenvironment in the diabetic bone defect region present a significant challenge to osteogenic regulation. Inspired by the distinctive characteristics of healing staged in diabetic bone defects and the structure-function relationship in the natural periosteum, we fabricated an electrospun bilayer biomimetic periosteum (Bilayer@E) to promote regeneration of diabetic bone defects. Here, the inner layer of biomimetic periosteum was fabricated using coaxial electrospinning fibers, with a shell incorporating zinc oxide nanoparticles (ZnO NPs) and a core containing silicon dioxide nanoparticles (SiO2 NPs) mimicking the cambium of periosteum; the outer layer consisted of randomly aligned electrospun fibers loaded with deferoxamine (DFO), simulating the fibrous layer of periosteum; finally, epigallocatechin-3-gallate (EGCG) was coated onto the bilayer membrane to obtain Bilayer@E. The presence of EGCG on the Bilayer@E surface efficiently triggers a phenotypic transition in macrophages, shifting them from an M1 proinflammatory state to an M2 anti-inflammatory state. Moreover, the sequential release of ZnO NPs, DFO, and SiO2 NPs exhibits antimicrobial characteristics while coordinating angiogenesis and promoting osteogenic mineralization in cells. Importantly, the biomimetic periosteum shows strong in vivo bone tissue and periosteal regeneration properties in diabetic rats. The integration of sequential drug release and immunomodulation, tailored to meet the specific healing requirements during bone regeneration, offers new insights for advancing the application of biomaterials in this field.
Keyword ：
biomimetic periosteum biomimetic periosteum bone regeneration bone regeneration diabetesmellitus diabetesmellitus electrospinning electrospinning immunomodulation immunomodulation
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| GB/T 7714 | Zhuang, Yu , Wu, Dingwei , Zhou, Lvyang et al. Electrospun Biomimetic Periosteum Promotes Diabetic Bone Defect Regeneration through Regulating Macrophage Polarization and Sequential Drug Release [J]. | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2025 , 11 (3) : 1690-1704 . |
| MLA | Zhuang, Yu et al. "Electrospun Biomimetic Periosteum Promotes Diabetic Bone Defect Regeneration through Regulating Macrophage Polarization and Sequential Drug Release" . | ACS BIOMATERIALS SCIENCE & ENGINEERING 11 . 3 (2025) : 1690-1704 . |
| APA | Zhuang, Yu , Wu, Dingwei , Zhou, Lvyang , Liu, Boyuan , Zhao, Xingkai , Yang, Jianmin et al. Electrospun Biomimetic Periosteum Promotes Diabetic Bone Defect Regeneration through Regulating Macrophage Polarization and Sequential Drug Release . | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2025 , 11 (3) , 1690-1704 . |
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In anterior cruciate ligament (ACL) repair methods, the continuous enzymatic erosion of synovial fluid can impede healing and potentially lead to repair failure, as well as exacerbate articular cartilage wear, resulting in joint degeneration. Inspired by the blood clot during medial collateral ligament healing, we developed a composite scaffold comprising collagen (1 %, w/v) and polyvinyl alcohol (5 %, w/v) combined with platelet-rich plasma (PRP). The composite scaffold provides a protective barrier against synovial erosion for the ruptured ACL, while simultaneously facilitating tissue repair, thereby enhancing the efficacy of ACL repair techniques. The composite scaffold is primarily formed through hydrogen bonding between molecular chains and physical cross- linking of microcrystalline regions using a simple cyclic freeze-thaw method, resulting in improved mechanical properties and an extended degradation period. The maximum tensile fracture load of the composite scaffold reached 5.99 +/- 0.30 N. The incorporation of PRP facilitates cell migration, proliferation, and blood vessel growth by enabling slow release of various growth factors. In vivo results demonstrate that this composite scaffold promotes rabbit hindlimb rupture ACL healing by stimulating fibroblast proliferation, collagen deposition, microvascular formation, and proprioceptor generation. Furthermore, it effectively reduces meniscus and cartilage wear while mitigating bone arthritis and joint degenerative diseases. Overall, our proposed composite scaffold holds great promise as a candidate for ACL healing.
Keyword ：
Anterior cruciate ligament Anterior cruciate ligament Biodegradable scaffold Biodegradable scaffold Collagen Collagen Platelet-rich plasma Platelet-rich plasma Polyvinyl alcohol Polyvinyl alcohol
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| GB/T 7714 | Sun, Xiaohan , Zhang, Nanxin , Chen, Longhui et al. Collagen/polyvinyl alcohol scaffolds combined with platelet-rich plasma to enhance anterior cruciate ligament repair [J]. | BIOMATERIALS ADVANCES , 2025 , 169 . |
| MLA | Sun, Xiaohan et al. "Collagen/polyvinyl alcohol scaffolds combined with platelet-rich plasma to enhance anterior cruciate ligament repair" . | BIOMATERIALS ADVANCES 169 (2025) . |
| APA | Sun, Xiaohan , Zhang, Nanxin , Chen, Longhui , Lai, Yuchao , Yang, Shasha , Li, Qiang et al. Collagen/polyvinyl alcohol scaffolds combined with platelet-rich plasma to enhance anterior cruciate ligament repair . | BIOMATERIALS ADVANCES , 2025 , 169 . |
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The redox homeostasis defense mechanism of tumor cells is one of the prime reasons for the unsatisfactory effect of photodynamic therapy (PDT). So far, little attention has been paid to this obstacle. In this work, we reported a synthesizing simple yet versatile nanogel (BCPS), synthesized by cystamine dihydrochloride functionalized sodium carboxymethylcellulose (CMC-SS), bovine serum albumin, and Phycocyanobilin self-assembly. The BCPS reduced the levels of glutathione molecules by reacting with glutathione, thereby interfering with intracellular redox homeostasis and enhancing the sensitivity of tumor cells to PDT. The BCPS was shown to possess excellent serum stability, high blood compatibility, low toxic side effects, and higher reactive oxygen species (ROS) utilization. After irradiation, the BCPS could significantly increase intracellular ROS level by approximately 1.6-fold and decrease the IC50 to HeLa cells by approximately 1.5-fold, compared to the pre-functional drugs BCP. This proposed strategy, based on increasing the utilization rate of ROS in tumor cells is promising for application potentials in tumor therapy.
Keyword ：
Green self-assembly Green self-assembly Long-circulating Long-circulating Photodynamic therapy Photodynamic therapy Redox-responsive Redox-responsive Tumor-targeted drug delivery Tumor-targeted drug delivery
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| GB/T 7714 | Liao, Wenqiang , Xiao, Siqi , Yang, Jianmin et al. Multifunctional nanogel based on carboxymethyl cellulose interfering with cellular redox homeostasis enhances phycocyanobilin photodynamic therapy [J]. | CARBOHYDRATE POLYMERS , 2024 , 323 . |
| MLA | Liao, Wenqiang et al. "Multifunctional nanogel based on carboxymethyl cellulose interfering with cellular redox homeostasis enhances phycocyanobilin photodynamic therapy" . | CARBOHYDRATE POLYMERS 323 (2024) . |
| APA | Liao, Wenqiang , Xiao, Siqi , Yang, Jianmin , Shi, Xianai , Zheng, Yunquan . Multifunctional nanogel based on carboxymethyl cellulose interfering with cellular redox homeostasis enhances phycocyanobilin photodynamic therapy . | CARBOHYDRATE POLYMERS , 2024 , 323 . |
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Platelet-rich plasma (PRP) has become an important regenerative therapy. However, the preparation method of PRP has not been standardized, and the optimal platelet concentration for PRP used in skin wound repair is unclear, leading to inconsistent clinical efficacy of PRP. Therefore, the development of standardized preparation methods for PRP and the investigation of the dose-response relationship between PRP with different platelet concentrations and tissue regeneration plays an important role in the development and clinical application of PRP technology. This study has developed an integrated blood collection device from blood drawing to centrifugation. Response surface methodology was employed to optimize the preparation conditions, ultimately achieving a platelet recovery rate as high as 95.74% for PRP (with optimal parameters: centrifugation force 1730x g, centrifugation time 10 min, and serum separation gel dosage 1.4 g). Both in vitro and in vivo experimental results indicate that PRP with a (2x) enrichment ratio is the most effective in promoting fibroblast proliferation and skin wound healing, with a cell proliferation rate of over 150% and a wound healing rate of 78% on day 7.
Keyword ：
high recovery rate high recovery rate optimal preparation method optimal preparation method platelet-poor plasma platelet-poor plasma platelet-rich plasma platelet-rich plasma skin restoration skin restoration
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| GB/T 7714 | Qiu, Mengjie , He, Yating , Zhang, Haijie et al. Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (17) . |
| MLA | Qiu, Mengjie et al. "Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25 . 17 (2024) . |
| APA | Qiu, Mengjie , He, Yating , Zhang, Haijie , Zheng, Yunquan , Shi, Xianai , Yang, Jianmin . Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (17) . |
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The management of severe full-thickness skin defect wounds remains a challenge due to their irregular shape, uncontrollable bleeding, high risk of infection, and prolonged healing period. Herein, an all-in-one OD/GM/QCS@Exo hydrogel was prepared with catechol-modified oxidized hyaluronic acid (OD), methylacrylylated gelatin (GM), and quaternized chitosan (QCS) and loaded with adipose mesenchymal stem cell-derived exosomes (Exos). Cross-linking of the hydrogel was achieved using visible light instead of ultraviolet light irradiation, providing injectability and good biocompatibility. Notably, the incorporation of catechol groups and multicross-linked networks in the hydrogels conferred strong adhesion properties and mechanical strength against external forces such as tensile and compressive stress. Furthermore, our hydrogel exhibited antibacterial, anti-inflammatory, and antioxidant properties along with wound-healing promotion effects. Our results demonstrated that the hydrogel-mediated release of Exos significantly promotes cellular proliferation, migration, and angiogenesis, thereby accelerating skin structure reconstruction and functional recovery during the wound-healing process. Overall, the all-in-one OD/GM/QCS@Exo hydrogel provided a promising therapeutic strategy for the treatment of full-thickness skin defect wounds through actively participating in the entire process of wound healing.
Keyword ：
all-in-one all-in-one exosomes exosomes multifunctionalhydrogel multifunctionalhydrogel quaternized chitosan quaternized chitosan visible light cross-linkable visible light cross-linkable
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| GB/T 7714 | Lv, Yicheng , Li, Liang , Zhang, Jingyuan et al. Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process [J]. | ACS APPLIED MATERIALS & INTERFACES , 2024 , 16 (20) : 25923-25937 . |
| MLA | Lv, Yicheng et al. "Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process" . | ACS APPLIED MATERIALS & INTERFACES 16 . 20 (2024) : 25923-25937 . |
| APA | Lv, Yicheng , Li, Liang , Zhang, Jingyuan , Li, Jingsi , Cai, Fengying , Huang, Yufeng et al. Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process . | ACS APPLIED MATERIALS & INTERFACES , 2024 , 16 (20) , 25923-25937 . |
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Currently, cisplatin resistance has been recognized as a multistep cascade process for its clinical chemotherapy failure. Hitherto, it remains challenging to develop a feasible and promising strategy to overcome the cascade drug resistance (CDR) issue for achieving fundamentally improved chemotherapeutic efficacy. Herein, a novel self-assembled nanoagent is proposed, which is constructed by Pt(IV) prodrug, cyanine dye (cypate), and gadolinium ion (Gd3+), for systematically conquering the cisplatin resistance by employing near-infrared (NIR) light activated mild-temperature hyperthermia in tumor targets. The proposed nanoagents exhibit high photostability, GSH/H+-responsive dissociation, preferable photothermal conversion, and enhanced cellular uptake performance. In particular, upon 785-nm NIR light irradiation, the generated mild temperature of approximate to 43 degrees C overtly improves the cell membrane permeability and drug uptake, accelerates the disruption of intracellular redox balance, and apparently enhances the formation of Pt-DNA adducts, thereby effectively overcoming the CDR issue and achieves highly improved therapeutic efficacy for cisplatin-resistant tumor ablation. A novel self-assembled nanoagent (Cy-Pt@HA NP) is developed to conquer the cascade drug resistance (CDR) issue of cisplatin. The proposed nanoagent presents NIR light-triggered mild hyperthermia, superior cellular uptake, and tumor-microenvironment-responsive dissociation for effective ablation of drug-resistant tumors, thereby opening a new avenue for addressing CDR issue and maximizing the platinum-based therapeutic efficacy for cancer treatment.image
Keyword ：
cascade cisplatin resistance cascade cisplatin resistance coordination self-assembly coordination self-assembly improved synergistic therapy improved synergistic therapy mild-temperature hyperthermia mild-temperature hyperthermia NIR light-responsive NIR light-responsive
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| GB/T 7714 | Chen, Mingmao , Fu, Yulei , Liu, Yan et al. NIR-Light-Triggered Mild-Temperature Hyperthermia to Overcome the Cascade Cisplatin Resistance for Improved Resistant Tumor Therapy [J]. | ADVANCED HEALTHCARE MATERIALS , 2024 , 13 (11) . |
| MLA | Chen, Mingmao et al. "NIR-Light-Triggered Mild-Temperature Hyperthermia to Overcome the Cascade Cisplatin Resistance for Improved Resistant Tumor Therapy" . | ADVANCED HEALTHCARE MATERIALS 13 . 11 (2024) . |
| APA | Chen, Mingmao , Fu, Yulei , Liu, Yan , Zhang, Baihe , Song, Xiaorong , Chen, Xinchun et al. NIR-Light-Triggered Mild-Temperature Hyperthermia to Overcome the Cascade Cisplatin Resistance for Improved Resistant Tumor Therapy . | ADVANCED HEALTHCARE MATERIALS , 2024 , 13 (11) . |
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BackgroundDe-novo-designed synthetic transcriptional regulators have great potential as the genetic parts for constructing complex multilayered gene circuits. The design flexibility afforded by advanced nucleic acid sequence design tools vastly expands the repertoire of regulatory elements for circuit design. In principle, the design space of synthetic regulators should allow for the construction of regulatory circuits of arbitrary complexity; still, the orthogonality and robustness of such components have not been fully elucidated, thereby limiting the depth and width of synthetic circuits.ResultsIn this work, we systematically explored the design strategy of synthetic transcriptional regulators, termed switchable transcription terminators. Specifically, by redesigning key sequence domains, we created a high-performance switchable transcription terminator with a maximum fold change of 283.11 upon activation by its cognate input RNA. Further, an automated design algorithm was developed for these elements to improve orthogonality for a complex multi-layered circuit construction. The resulting orthogonal switchable transcription terminators could be used to construct a three-layer cascade circuit and a two-input three-layer OR gate.ConclusionsWe demonstrated a practical strategy for designing standardized regulatory elements and assembling modular gene circuits, ultimately laying the foundation for the streamlined construction of complex synthetic gene circuits.
Keyword ：
Computational design Computational design Multilayered cascades Multilayered cascades Synthetic logic circuits Synthetic logic circuits Synthetic riboregulator Synthetic riboregulator Transcriptional regulation Transcriptional regulation
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| GB/T 7714 | Zhao, Mingming , Kim, Jeongwon , Jiao, Jiayan et al. Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems [J]. | JOURNAL OF BIOLOGICAL ENGINEERING , 2024 , 18 (1) . |
| MLA | Zhao, Mingming et al. "Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems" . | JOURNAL OF BIOLOGICAL ENGINEERING 18 . 1 (2024) . |
| APA | Zhao, Mingming , Kim, Jeongwon , Jiao, Jiayan , Lim, Yelin , Shi, Xianai , Guo, Shaobin et al. Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems . | JOURNAL OF BIOLOGICAL ENGINEERING , 2024 , 18 (1) . |
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Resveratrol (Res) is a natural polyphenol exhibiting anti-oxidant and anti-inflammatory activity. However, the applications of Res have been limited due to its low stability and water solubility. To enhance the bioaccessibility of Res, unfolding bovine serum albumin-modified selenium nanoparticles (UBSA@SeNPs) encapsulated within chitosan (CS)-coated Pickering emulsions (CS-UBSA@SeNPs-PE) were used to load Res. The results showed that Res-loaded CS(0.06 %)-UBSA@SeNPs-PE has small droplet size (16.13 mu m), high gel properties and excellent antioxidant properties. During the simulated digestion process, CS reduced the release rate of Res from Resloaded CS(0.06 %)-UBSA@SeNPs-PE (42.27 %) to reach a slow release effect. Importantly, Res could quickly release from CS-UBSA@SeNPs-PE within intestinal fluid or in the presence of chitosanase. In simulated absorption experiments, the intestinal permeability of Res in Res-loaded CS(0.06 %)-UBSA@SeNPs-PE were enhanced by 292.31 % compare to Res-loaded CS(0 %)-UBSA@SeNPs-PE. In pharmacokinetic studies, Resloaded CS(0.06 %)-UBSA@SeNPs-PE had an area under the curve (AUC) up to 3467.99 +/- 127.43 ng*h/mL. Furthermore, CS also improved the mucoadhesive nature of UBSA@SeNPs-PE, resulting in a gut-retention time of Res-loaded CS(0.06 %)-UBSA@SeNPs-PE that reached up to 60 h. In conclusion, CS-UBSA@SeNPs-PE can serve as an effective oral delivery system for improving the bioaccessibility of Res.
Keyword ：
Bioaccessibility Bioaccessibility Chitosan crosslinking Chitosan crosslinking Pickering emulsion Pickering emulsion Resveratrol Resveratrol Selenium nanoparticle Selenium nanoparticle
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| GB/T 7714 | Mei, Jingtao , Shi, Xianai , Chen, Mingmao et al. Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 289 . |
| MLA | Mei, Jingtao et al. "Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 289 (2024) . |
| APA | Mei, Jingtao , Shi, Xianai , Chen, Mingmao , Cui, Yanan , Fang, Chaoping , Yang, Licong . Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2024 , 289 . |
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