The　compounds　that　promote　calcium　absorption　and　osteogenesis　may　have　great　potential　in　controlling　adipogenesis　and　obesity.　In　this　study,　the　glycosylated　peptides-calcium　chelate　prepared　from　Crimson　Snapper　scale　protein　hydrolysates　(CSPHs)　and　xylooligosaccharide　(XOS)　via　transglutaminase　pathway　with　calcium　absorption　promoting　and　osteogenic　activities,　named　XOS-CSPHs-Ca-TG,　were　used　to　explore　the　potential　prebiotic　effect　and　lipid-lowering　activity　on　3T3-L1　cells.　The　presence　of　XOS-CSPHs-Ca-TG　in　pure　cultures　could　significantly　promote　the　proliferation　of　probiotics　(Bifidobacterium　adolescentis　and　Lactobacillus　acidophilus),　enabling　them　to　enter　the　logarithmic　growth　phase　2-4　h　earlier.　Results　of　in　vitro　fermentation　of　mice　intestinal　flora　further　indicated　that　XOS-CSPHs-Ca-TG　increased　the　number　of　beneficial　bacteria　such　as　Bifidobacterium　and　Lactobacillus　by　11.57%　and　7.63%,　respectively,　and　boosted　the　production　of　short-chain　fatty　acids,　thereby　improving　the　colonization　ability　of　the　intestinal　microbiota.　Results　of　quantitative　real-time　polymerase　chain　reaction　confirmed　that　XOS-CSPHs-Ca-TG　achieved　lipid-lowering　effects　by　accelerating　calcium　influx　and　activating　the　mitogen-activated　protein　kinase/extracellular　signal-regulated　kinases　signaling　pathway,　thereby　inhibiting　the　gene　expressions　of　the　downstream　peroxisome　proliferator-activated　receptors　gamma　and　CCAAT/enhancer-binding　protein　alpha,　and　further　attenuating　the　secretion　of　adipokines　and　controlling　the　expression　of　key　lipases.　These　results　suggest　that　glycosylated　peptides-calcium　chelates　are　potentially　functional　foods　in　lipid　metabolism-related　disease　prevention.　Glycosylated　peptide-calcium　chelate　prepared　through　transglutaminase　pathway　improved　the　intestinal　microbiota　structure　of　calcium-deficient　mice.　Glycosylated　peptides-calcium　chelates　inhibited　adipogenesis　by　attenuating　the　expression　of　PPAR　gamma　and　C/EBP　alpha,　reducing　the　secretion　of　adipokines,　and　controlling　the　expression　of　key　lipases.　Lipid-lowering　effect　of　XOS-CSPHs-Ca-TG　was　by　accelerating　calcium　influx　and　activating　the　MAPK/ERK　signaling　pathway.image