This　study　developed　a　new　“capture　and　killing”　antibacterial　approach　for　efficient　elimination　of　foodborne　pathogens.　Fe3O4-Chitosan　(CS)/polyvinyl　alcohol　(PVA)　nanofibrous　films　with　improved　antibacterial　and　mechanical　properties　were　fabricated　by　a　simple,　environmentally　friendly,　and　cost-effective　electrospinning　technique.　The　relationship　between　the　physical　properties　(viscosity,　surface　tension,　and　conductivity)　and　spinnability　of　CS/PVA　as　fiber　forming　matrix　was　explored.　Electrospun　Fe3O4-CS/PVA　films　(0.03–0.12　mm)　with　smooth　and　bead-free　nanofibrous　structures　(145–169　nm)　were　successfully　obtained.　Compared　with　the　film　electrospun　from　neat　CS/PVA,　incorporating　Fe3O4　nanoparticles　(NPs)　(1.25–5　wt%)　in　CS/PVA　nanofibrous　film　promoted　bacterial　attachment　and　increased　the　final　inactivated　efficiency,　showing　a　difference　with　Fe3O4　loading　and　bacterial　strain,　which　had　the　highest　value　against　Escherichia　coli　(E.　coli)　and　Staphyloccus　aureus　(S.　aureus)　being　90　%　and　66.30　%,　respectively.　The　tensile　strength　and　elongation　at　break　of　Fe3O4-CS/PVA　films　enhanced　by　46–192　%　and　92–141　%,　respectively.　Results　of　the　cytotoxicity　test　indicated　that　the　resulting　films　had　high　biocompatibility.　These　promising　findings　provide　a　novel　strategy　for　effective　foodborne　pathogens　elimination,　which　could　apply　to　sterilizing　and　food　packaging　to　extend　the　shelf　life　of　liquid　food.　©　2023