Exploring　and　understanding　the　interaction　of　changes　in　the　activities　of　various　enzymes,　such　as　proteases,　phosphatases,　and　oxidoreductases　with　tumor　invasion,　proliferation,　and　metastasis　is　of　great　significance　for　early　cancer　diagnosis.　To　detect　the　activity　of　tumor-related　enzymes,　various　molecular　probes　have　been　developed　with　different　imaging　methods,　including　optical　imaging,　photoacoustic　imaging　(PAI),　magnetic　resonance　imaging,　positron　emission　tomography,　and　so　on.　In　this　review,　we　first　describe　the　biological　functions　of　various　enzymes　and　the　selectively　recognized　chemical　linkers　or　groups.　Subsequently,　we　systematically　summarize　the　design　mechanism　of　imaging　probes　and　different　imaging　methods.　Finally,　we　explore　the　challenges　and　development　prospects　in　the　field　of　enzyme　activity　detection.　This　comprehensive　review　will　provide　more　insight　into　the　design　and　development　of　enzyme　activated　molecular　probes.