Purpose　In　this　study,　a　simulator　for　training　lateral　ventricular　puncture　(LVP)　was　developed　using　three-dimensional　(3D)　printing　technology,　and　its　function　of　improving　the　skills　of　LVP　in　young　interns　was　evaluated.　Methods　A　virtual　3D　craniocerebral　simulator　of　a　51-year-old　female　patient　with　hydrocephalus　was　reconstructed　with　3D　printing　technology.　The　anatomical　and　practical　validity　were　assessed　by　all　interns　on　a　13-item　Likert　scale.　The　usefulness　of　this　simulator　was　evaluated　once　a　week　by　two　neurosurgeons,　based　on　the　performance　of　the　interns,　using　the　objective　structured　assessment　of　technical　skills　(OSATS)　scale.　Results　The　Likert　scale　showed　that　all　participants　agreed　with　the　overall　appearance　of　the　simulator.　Also,　the　authenticity　of　the　skull　was　the　best,　followed　by　the　lateral　ventricles,　analog　generation　system　of　intraventricular　pressure,　cerebrum,　and　the　scalp.　This　simulator　could　help　the　participants＇　learning　about　the　anatomy　of　the　lateral　ventricle,　effective　training,　and　repeating　the　steps　of　LVP.　During　training,　the　interns＇　ratio　of　success　in　LVP　elevated　gradually.　At　each　evaluation　stage,　all　mean　performance　scores　for　each　measure　based　on　the　OSATS　scale　were　higher　than　the　previous.　Conclusions　The　3D　printed　simulator　for　LVP　training　provided　both　anatomical　and　practical　validity,　and　enabled　young　doctors　to　master　the　LVP　procedures　and　skills.