Beauveria　bassiana　is　a　critical　entomopathogenic　fungus　for　pest　biocontrol,　whose　efficiency　depends　on　fungal　development　and　stress　resistance.　Unlike　its　revealed　location　in　plasma　membrane　patches　in　other　organisms,　B.　bassiana　Sur7　specifically　localized　in　vacuoles.　This　vacuolar　Sur7　was　previously　demonstrated　to　affect　stress　tolerance,　hyphal　development　and　virulence.　There,　however,　remain　more　mechanistic　details　to　be　explored.　In　this　study,　transcriptomics　and　metabolomics　were　applied　to　investigate　the　mechanism　of　vacuolar　Sur7.　Analyses　of　transcriptomics　and　metabolomics　displayed　many　differentially　expressed　genes　and　abundant　metabolites　in　response　to　Sur7　loss,　respectively.　Together　with　genes　associated　with　vacuolar　biofunction　(including　transportation　and　hydrolysis),　the　altered　metabolites　contributed　to　cell　wall　construction　and　stress　resistance.　Particularly,　an　N-acetylglucosamine-associated　Brg1/Nrg1　pathway　was　enriched　and　partially　affected　by　Sur7.　Absence　of　Sur7　changed　the　expression　level　of　Brg1/Nrg1　pathway-related　transcript　factors,　which　interfered　with　downstream　phenotype　of　sporulation.　In　addition,　Sur7　was　involved　in　the　accumulation　of　sphingoid　bases,　which　may　affect　sphingolipid-related　signaling　pathway.　Although　experimental　evidence　is　further　required,　our　studies　provide　a　preliminary　framework　for　future　exploring　the　regulatory　mechanism　of　Sur7,　and　give　a　new　version　of　metabolic　agency　connecting　Sur7　and　downstream　signaling　pathway.