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Abstract ：
N-acetylglucosamine (GlcNAc), a key component of fungal cell walls and insect cuticles, is an important signal to activate fungal response during entomopathogen-insect interactions. Research on Ngs1, the only identified GlcNAc sensor and transducer, has been primarily restricted to Candida species. Although our previous work identified an Ngs1 homology in Beauveria bassiana, its physiological functions in entomopathogenic fungi remain largely unexplored. In this study, we unveiled the sub-localization of Ngs1 in the nucleolus. Further transcriptomic analysis revealed that Ngs1 plays a crucial role in vegetative growth, fungal development, and cellwall construction by acting as a transcriptional mediator, particularly influencing carbon metabolism in response to insect cuticle stimulation. The absence of Ngs1 compromised vegetative growth across various carbon sources by downregulating expressions of key catalytic enzymes. Conversely, Ngs1 deficiency enhanced transcription levels of oxidative phosphorylation, leading to increased ATP and reactive oxygen species (ROS) production. Despite higher ATP levels, Ngs1-deletion mutants exhibited reduced asexual development and hyphal germination, primarily due to the function of Ngs1 in the central developmental pathway and Brg1/Nrg1dependent pathway. Additionally, the downregulation of N-glycan biosynthesis in Delta Ngs1 diminished cell wall components, resulting in decreased cell wall resistance to lysis and impaired fungal development. These findings advance our understanding of the regulatory role of Ngs1 in B. bassiana during host interactions and provide a theoretical foundation for engineering fungi to maintain or even enhance pesticidal activity.
Keyword ：
Beauveria bassiana Beauveria bassiana Biocontrol traits Biocontrol traits Entomopathogenic fungi Entomopathogenic fungi N -acetylglucosamine sensor N -acetylglucosamine sensor Transcriptome Transcriptome
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| GB/T 7714 | Cui, Zhou , Yang, Wu-Wei-Jie , Yang, Zhi-Hao et al. N-acetylglucosamine sensor, Ngs1 contributes to Beauveria bassiana vegetative growth, oxidative phosphorylation, fungal development, and cell wall integrity during entomopathogen-insect interaction [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 208 . |
| MLA | Cui, Zhou et al. "N-acetylglucosamine sensor, Ngs1 contributes to Beauveria bassiana vegetative growth, oxidative phosphorylation, fungal development, and cell wall integrity during entomopathogen-insect interaction" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 208 (2025) . |
| APA | Cui, Zhou , Yang, Wu-Wei-Jie , Yang, Zhi-Hao , Zhang, Long-Bin , Guan, Yi . N-acetylglucosamine sensor, Ngs1 contributes to Beauveria bassiana vegetative growth, oxidative phosphorylation, fungal development, and cell wall integrity during entomopathogen-insect interaction . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 208 . |
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The white rot fungus Cerrena unicolor 87613 has been previously shown to be a promising resource in laccase production, an enzyme with significant biotechnological applications. Conventional methods face technical challenges in improving laccase activity. Attempts are still being made to develop novel approaches for further enhancing laccase activity. This study aimed to understand the regulation of laccase activity in C. unicolor 87613 for a better exploration of the novel approach. Transcriptomic and metabolomic analyses were performed to identify key genes and metabolites involved in extracellular laccase activity. The findings indicated a strong correlation between the glutathione metabolism pathway and laccase activity. Subsequently, experimental verifications were conducted by manipulating the pathway using chemical approaches. The additive reduced glutathione (GSH) dose-dependently repressed laccase activity, while the GSH inhibitors (APR-246) and reactive oxygen species (ROS) inducer (H2O2) enhanced laccase activity. Changes in GSH levels could determine the intracellular redox homeostasis in interaction with ROS and partially affect the expression level of laccase genes in C. unicolor 87613 in turn. In addition, GSH synthetase was found to mediate GSH abundance in a feedback loop. This study suggests that laccase activity is negatively influenced by GSH metabolism and provides a theoretical basis for a novel strategy to enhance laccase activity by reprogramming glutathione metabolism at a specific cultivation stage.
Keyword ：
Cerrena unicolor Cerrena unicolor glutathione metabolism glutathione metabolism laccase activity laccase activity metabolome metabolome transcriptome transcriptome
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| GB/T 7714 | Zhang, Long-Bin , Qiu, Ting-Ting , Qiu, Xiu-Gen et al. Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613 [J]. | MICROBIOLOGY SPECTRUM , 2024 , 12 (2) . |
| MLA | Zhang, Long-Bin et al. "Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613" . | MICROBIOLOGY SPECTRUM 12 . 2 (2024) . |
| APA | Zhang, Long-Bin , Qiu, Ting-Ting , Qiu, Xiu-Gen , Yang, Wu-Wei-Jie , Ye, Xiu-Yun , Meng, Chun . Transcriptomic and metabolomic analysis unveils a negative effect of glutathione metabolism on laccase activity in Cerrena unicolor 87613 . | MICROBIOLOGY SPECTRUM , 2024 , 12 (2) . |
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As N-acetylglucosamine (GlcNAc) ubiquitously exists in both insect cuticle and fungal cell walls, the GlcNAc sensor (Ngs1) potentially plays important roles in the interactions between entomopathogenic fungi and their insect hosts. However, the roles of the Ngs1 derived from the entomopathogens in response to the host's cuticle remain completely unexplored. In this study, a putative Ngs1 homolog was identified in the entomopathogenic fungus Beauveria bassiana. Deletion of Ngs1 significantly reduced virulence towards Galleria mellonella larvae either through cuticle infection (by 23%) or by bypassing the cuticle (by 44%). To investigate the role of Ngs1 in fungal virulence, an analysis of the transcriptome induced by Locusta migratoria exoskeleton was conducted, highlighting the regulatory mechanism of Ngs1 in carbohydrate metabolic process, particularly chitin metabolism and GlcNAc metabolism. Consistent with the transcriptomic data, Ngs1-deletion mutants showed reduced activities of both secreted chitinase (17% reduction) and Pr1 protease (35% reduction). Loss of Ngs1 down-regulated the transcript levels of GlcNAc-catabolism genes, resulting in a 17% decrease in fungal growth on GlcNAc-supported media. Furthermore, Ngs1 deficiency attenuated the fungal response to GlcNAc, leading to the alteration of fungal resistance to diverse stress cues. All of these changes contribute to the reduction in virulence in Ngs1-deficient B. bassiana. These findings support that Ngs1 plays a critical role in responding to insect-derived GlcNAc, affecting the production of cuticle-degrading enzymes to penetrate insect epidermis, GlcNAc-induced changes of stress resistance, and contribute to the fungal virulence against insects.
Keyword ：
Entomopathogenic fungi Entomopathogenic fungi N -acetylglucosamine response N -acetylglucosamine response N -acetylglucosamine sensor N -acetylglucosamine sensor Pathogenic traits Pathogenic traits Transcriptome Transcriptome
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| GB/T 7714 | Zhang, Long-Bin , Yang, Zhi-Hao , Yang, Wu-Wei-Jie et al. A novel fungal sensor (Ngs1) of N-acetylglucosamine (GlcNAc) mediates the fungal response to GlcNAc in the interaction between entomopathogenic Beauveria bassiana and insect host [J]. | JOURNAL OF INVERTEBRATE PATHOLOGY , 2024 , 207 . |
| MLA | Zhang, Long-Bin et al. "A novel fungal sensor (Ngs1) of N-acetylglucosamine (GlcNAc) mediates the fungal response to GlcNAc in the interaction between entomopathogenic Beauveria bassiana and insect host" . | JOURNAL OF INVERTEBRATE PATHOLOGY 207 (2024) . |
| APA | Zhang, Long-Bin , Yang, Zhi-Hao , Yang, Wu-Wei-Jie , Guan, Yi . A novel fungal sensor (Ngs1) of N-acetylglucosamine (GlcNAc) mediates the fungal response to GlcNAc in the interaction between entomopathogenic Beauveria bassiana and insect host . | JOURNAL OF INVERTEBRATE PATHOLOGY , 2024 , 207 . |
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Rad6 functions as a ubiquitin-conjugating protein that regulates cellular processes in many fungal species. However, its role in filamentous entomopathogenic fungi remains poorly understood. This study characterizes Rad6 in Beauveria bassiana, a filamentous fungus widely employed as a critical fungicide globally. The results demonstrate a significant association between Rad6 and conidial properties, heat shock response, and UV-B tolerance. Concurrently, the mutant strain exhibited heightened sensitivity to oxidative stress, cell wall interfering agents, DNA damage stress, and prolonged heat shock. Furthermore, the absence of Rad6 significantly extended the median lethal time (LT50) of Galleria mellonella infected by B. bassiana. This delay could be attributed to reduced Pr1 proteases and extracellular cuticle-degrading enzymes, diminished dimorphic transition rates, and dysregulated antioxidant enzymes. Additionally, the absence of Rad6 had a more pronounced effect on genetic information processing, metabolism, and cellular processes under normal conditions. However, its impact was limited to metabolism in oxidative stress. This study offers a comprehensive understanding of the pivotal roles of Rad6 in conidial and hyphal stress tolerance, environmental adaptation, and the pathogenesis of Beauveria bassiana.
Keyword ：
Beauveria bassiana Beauveria bassiana DNA damaging DNA damaging filamentous fungi filamentous fungi Rad6 Rad6 stress tolerance stress tolerance virulence virulence
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| GB/T 7714 | Guan, Yi , He, Haomin , Guo, Yuhan et al. Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana [J]. | VIRULENCE , 2024 , 15 (1) . |
| MLA | Guan, Yi et al. "Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana" . | VIRULENCE 15 . 1 (2024) . |
| APA | Guan, Yi , He, Haomin , Guo, Yuhan , Zhang, Longbin . Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana . | VIRULENCE , 2024 , 15 (1) . |
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Rad6 is a canonical ubiquitin-conjugating enzyme known for its role in regulating chromosome-related cellular processes in yeast and has been proven to have multiple functions in Beauveria bassiana, including insect-pathogenic lifestyle, UV damage repair, and conidiation. However, previous studies have only reported the key role of Rad6 in regulating conidial production in a nutrient-rich medium, without any deep mechanism analyses. In this study, we found that the disruption of Rad6 leads to a profound reduction in conidial production, irrespective of whether the fungus is cultivated in nutrient-rich or nutrient-poor environments. The absence of rad6 exerts a suppressive effect on the transcription of essential genes in the central developmental pathway, namely, brlA, abaA, and wetA, resulting in a direct downregulation of conidiation capacity. Additionally, mutant strains exhibited a more pronounced decline in both conidial generation and hyphal development when cultured in nutrient-rich conditions. This observation correlates with the downregulation of the central developmental pathway (CDP) downstream gene vosA and the upregulation of flaA in nutrient-rich cultures. Moreover, single-transcriptomics analyses indicated that irregularities in biotin metabolism, DNA repair, and tryptophan metabolism are the underlying factors contributing to the reduced conidial production. Comprehensive dual transcriptomics analyses pinpointed abnormal biotin metabolism as the primary cause of conidial production decline. Subsequently, we successfully restored conidial production in the Rad6 mutant strain through the supplementation of biotin, further confirming the transcriptomic evidence. Altogether, our findings underscore the pivotal role of Rad6 in influencing biotin metabolism, subsequently impacting the expression of CDP genes and ultimately shaping the asexual life cycle of B. bassiana.
Keyword ：
Beauveria bassiana Beauveria bassiana biotin biotin conidiation conidiation entomopathogenic fungi entomopathogenic fungi Rad6 Rad6
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| GB/T 7714 | Guo, Yuhan , He, Haomin , Guan, Yi et al. Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana [J]. | JOURNAL OF FUNGI , 2024 , 10 (9) . |
| MLA | Guo, Yuhan et al. "Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana" . | JOURNAL OF FUNGI 10 . 9 (2024) . |
| APA | Guo, Yuhan , He, Haomin , Guan, Yi , Zhang, Longbin . Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana . | JOURNAL OF FUNGI , 2024 , 10 (9) . |
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Beauveria bassiana is a popular and eco-friendly biopesticide. During its pathogen-pest interaction, both N-acetylglucosamine (GlcNAc) catabolism and anabolism are crucial for nutrient supply and cell-wall construction. The initiation of GlcNAc metabolism relies on the catalysis of GlcNAc kinase, which has been extensively studied in the human pathogen Candida albicans. However, the physiological function of GlcNAc kinase remains poorly understood in entomopathogenic fungi. In the present study, a GlcNAc kinase homolog was identified and designated as BbHxk1 in B. bassiana. Deletion of BbHxk1 resulted in viable but reduced vegetative growth on various carbon sources. Delta BbHxk1 mutants displayed severe defects in cell wall integrity, making them more susceptible to cell wall stress cues. Furthermore, the absence of BbHxk1 resulted in an increase in conidial yield and blastospore production, and a faster rate of germination and filamentation, potentially attributed to higher intracellular ATP levels. BbHxk1 deficiency led to a reduction in the activities of cuticle-degrading enzymes, which might contribute to the attenuated pathogenicity specifically through cuticle penetration rather than hemocoel infection towards Galleria mellonella larvae. Being different from C. albicans Hxk1, which facultatively acts as a catalyzing enzyme and transcriptional regulator, BbHxk1 primarily acts as a catalyzing enzyme and metabolic regulator. The altered metabolomic profiling correlated with the phenotypic defects in Delta BbHxk1 mutants, further implicating a potential metabolism-dependent mechanism of BbHxk1 in mediating physiologies of B. bassiana. These findings not only unveil a novel role for GlcNAc kinase in B. bassiana, but also provide a solid theoretical basis to guide metabolic reprogramming in order to maintain or even enhance the efficiency of fungi for practical applications.
Keyword ：
Beauveria bassiana Beauveria bassiana Filamentous entomopathogenic fungus Filamentous entomopathogenic fungus Metabolomics Metabolomics N -acetylglucosamine kinase N -acetylglucosamine kinase Physiological phenotypes Physiological phenotypes
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| GB/T 7714 | Zhang, Long-Bin , Yang, Wu-Wei-Jie , Yang, Zhi-Hao et al. N-acetylglucosamine kinase (BbHxk1) has pleiotropic effects on vegetative growth, cell wall integrity, morphological transition, cuticle infection, and metabolic modulation in the biological pesticide Beauveria bassiana [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2024 , 203 . |
| MLA | Zhang, Long-Bin et al. "N-acetylglucosamine kinase (BbHxk1) has pleiotropic effects on vegetative growth, cell wall integrity, morphological transition, cuticle infection, and metabolic modulation in the biological pesticide Beauveria bassiana" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 203 (2024) . |
| APA | Zhang, Long-Bin , Yang, Wu-Wei-Jie , Yang, Zhi-Hao , Guan, Yi . N-acetylglucosamine kinase (BbHxk1) has pleiotropic effects on vegetative growth, cell wall integrity, morphological transition, cuticle infection, and metabolic modulation in the biological pesticide Beauveria bassiana . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2024 , 203 . |
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Rho4 is a member of the Rho-family small GTPases. In this study, we revealed the function of Rho4 and explored its mechanism involved in intracellular redox homeostasis in Beauveria bassiana, one of the most widely utilized filamentous entomopathogenic fungi. The disruption of Rho4 in B. bassiana resulted in significant phenotypic changes, such as fungal virulence, growth rate on different media, thermotolerance, germination, and conidiation. Integrated analysis of proteomic and transcriptomic data unveiled differential expression patterns of various redox-related genes and proteins in Delta rho4, including the down-regulation of GST shown in proteomic and transcriptomic data, and the down-regulated gene expression levels of NOX, SOD, CAT, and GR in the transcriptome. Based on the bi-omics analysis, we focused on the impact of Rho4 in maintaining intracellular redox homeostasis. A decreased ROS content observed in Delta rho4 might be attributed to the reduced NOX activity, which subsequently affects the GSH-producing/consuming metabolisms, with the attenuated activities of GR and GST. The imbalanced redox homeostasis also resulted in the reduced enzyme activities of SOD and CAT. Exogenous oxides could partially complement the ROS level and rescue the growth defect in Delta rho4 to a certain extent. Besides, BbGDI was initially identified as an interacting protein of Rho4 in entomopathogenic fungi. Our results provide a comprehensive understanding of the function and regulating mechanism of Rho4 in B. bassiana.
Keyword ：
Beauveria bassiana Beauveria bassiana Proteomic analysis Proteomic analysis Redox homeostasis Redox homeostasis Rho4 Rho4 Small GTPase Small GTPase Transcriptomic analysis Transcriptomic analysis
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| GB/T 7714 | Zou, Zhenyu , Chen, Xiaonuo , Weng, Xiaojun et al. Rho4 interacts with BbGDI and is essential for the biocontrol potential of Beauveria bassiana by maintaining intracellular redox homeostasis [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2024 , 205 . |
| MLA | Zou, Zhenyu et al. "Rho4 interacts with BbGDI and is essential for the biocontrol potential of Beauveria bassiana by maintaining intracellular redox homeostasis" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 205 (2024) . |
| APA | Zou, Zhenyu , Chen, Xiaonuo , Weng, Xiaojun , Guo, Yuhan , Guan, Yi , Zhang, Longbin . Rho4 interacts with BbGDI and is essential for the biocontrol potential of Beauveria bassiana by maintaining intracellular redox homeostasis . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2024 , 205 . |
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Phenolic compounds are widely distributed in nature and industrial environment, and their detoxifi-cation or bioactive enhancement is of great value to environmental protection and industrial develop-ment. Laccases are multicopper oxidases that catalyse the oligo-or polymerisation of phenolic compounds. Identifying new laccase producers and investigating their application potential are of great importance. In this study, a white-rot fungus, Trametes hirsuta EZ1, with significantly high laccase pro-ductivity was isolated. The optimum conditions were studied for the maximum fermentation of extra-cellular laccase, which was achieved at 150 U/mL with a medium containing 10% strain EZ1, 7% maltodextrin, 1.5% peptone, and 0.5 mM Cu2+, and incubation at initial pH 6.0, 32 degrees C, and 180 rpm for nine days. Subsequently, a 70-kDa laccase was purified that showed activity over a wide range of tem-perature and pH, sensitivity to many metal ions and sodium dodecyl sulphate, and high tolerance to organic solvents. Purified laccase showed a significant unreported effect by catalysing catechol or ferulic acid into dimers, trimers, and tetramers or caffeic acid into dimers, trimers, tetramers, and pentamers. The oligomeric mixtures exhibited increased antioxidative capacity compared to that of each parent monomer, except for caffeic acid derivatives. Our study offers a novel strain source for laccase production and broadens its application in the enhancement of bioactive compounds.(c) 2022 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Keyword ：
Caffeic acid Caffeic acid Catechol Catechol Ferulic acid Ferulic acid Laccase Laccase Oligomerisation Oligomerisation Trametes hirsuta Trametes hirsuta
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| GB/T 7714 | Zhang, Long-Bin , Deng, Zhi-Qiang , Qiu, Ting-Ting et al. Characterisation of a laccase isolated from Trametes hirsuta and its application in the oligomerisation of phenolic compounds [J]. | FUNGAL BIOLOGY , 2023 , 127 (1-2) : 872-880 . |
| MLA | Zhang, Long-Bin et al. "Characterisation of a laccase isolated from Trametes hirsuta and its application in the oligomerisation of phenolic compounds" . | FUNGAL BIOLOGY 127 . 1-2 (2023) : 872-880 . |
| APA | Zhang, Long-Bin , Deng, Zhi-Qiang , Qiu, Ting-Ting , Yang, Wu-Wei-Jie , Zhu, Fan , Ye, Xiu-Yun . Characterisation of a laccase isolated from Trametes hirsuta and its application in the oligomerisation of phenolic compounds . | FUNGAL BIOLOGY , 2023 , 127 (1-2) , 872-880 . |
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Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority. Cerrena species have been shown to be promising basidiomycete candidates for laccase production. Although two sets of Cerrena genome data have been publicly published, no comprehensive bioinformatics study of laccase gene family in C. unicolor has been reported, particularly concerning the analysis of their three-dimensional (3D) structures and molecular docking to substrates, like ABTS and aflatoxin B1 (AFB1). Results In this study, we conducted a comprehensive genome-wide analysis of laccase gene family in C. unicolor 87613. We identified eighteen laccase genes (CuLacs) and classified them into three clades using phylogenetic analysis. We characterized these laccases, including their location in contig 5,6,9,12,15,19,26,27, gene structures of different exon-intron arrangements, molecular weight ranging from 47.89 to 141.41 kDa, acidic pI value, 5-15 conserved protein motifs, signaling peptide of extracellular secretion (harbored by 13 CuLacs) and others. In addition, the analysis of cis-acting element in laccase promoters indicated that the transcription response of CuLac gene family was regulatable and complex under different environmental cues. Furthermore, analysis of transcription pattern revealed that CuLac8, 12 and CuLac2, 13 were the predominant laccases in response to copper ions or oxidative stress, respectively. Finally, we focused on the 3D structure analysis of CuLac proteins. Seven laccases with extra transmembrane domains or special sequences were particularly interesting. Predicted structures of each CuLac protein with or without these extra sequences showed altered interacting amino acid residues and binding sites, leading to varied affinities to both ABTS and AFB1. As far as we know, it is the first time to discuss the influence of the extra sequence on laccase's affinity to substrates. Conclusions Our findings provide robust genetic data for a better understanding of the laccase gene family in C. unicolor 87613, and create a foundation for the molecular redesign of CuLac proteins to enhance their industrial applications.
Keyword ：
3D structure 3D structure Cerrena unicolor Cerrena unicolor Gene family Gene family Genome Genome Laccase Laccase Molecular docking Molecular docking Transcriptional response Transcriptional response
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| GB/T 7714 | Zhang, Long-Bin , Yang, Wu-Wei-Jie , Qiu, Ting-Ting . Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation [J]. | BMC GENOMICS , 2023 , 24 (1) . |
| MLA | Zhang, Long-Bin et al. "Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation" . | BMC GENOMICS 24 . 1 (2023) . |
| APA | Zhang, Long-Bin , Yang, Wu-Wei-Jie , Qiu, Ting-Ting . Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation . | BMC GENOMICS , 2023 , 24 (1) . |
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为阐明BbRho5对球孢白僵菌生防潜能的作用,构建了Bbrho5单基因敲除菌株ΔBbrho5,以野生型菌株WT作为对照,在不同培养基上测定菌落生长速率,并测定了菌株对多菌灵胁迫耐受性及对大蜡螟幼虫体壁侵染能力.进一步获取和分析了ΔBbrho5和WT细胞内基因转录组数据.结果表明,BbRho5蛋白功能缺陷显著抑制球孢白僵菌菌丝生长速率,同时微弱影响其多菌灵胁迫抗逆性及生防能力.相较于WT,ΔBbrho5中具有770个差异表达基因(DEGs),其中上调基因395个,下调基因375个.GO分析显示,ΔBbrho5 VS WT中DEGs主要富集于氧化还原酶活力(oxidoreductase activity)和单加氧酶活力(monooxygenase activity)功能.KEGG通路富集结果显示,DEGs主要富集于氮代谢及多种氨基酸代谢通路.在氮代谢通路中富集到7个功能基因,其中有5个上调,2个下调,说明敲除菌株可能采用增强氮源利用及谷氨酸合成以应对Bbrho5缺陷引起的生长迟缓.以上研究结果揭示了球孢白僵菌中小GTP酶BbRho5对球孢白僵菌生长速率具有重要影响,且氮代谢和氨基酸代谢可能为其重要的响应代谢通路.
Keyword ：
BbRho5 BbRho5 小GTP酶 小GTP酶 球孢白僵菌 球孢白僵菌 生长速率 生长速率 转录组 转录组
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| GB/T 7714 | 关怡 , 李新 , 王定一 et al. BbRho5对球孢白僵菌生长速率的作用研究 [J]. | 生物技术通报 , 2022 , 38 (2) : 132-140 . |
| MLA | 关怡 et al. "BbRho5对球孢白僵菌生长速率的作用研究" . | 生物技术通报 38 . 2 (2022) : 132-140 . |
| APA | 关怡 , 李新 , 王定一 , 杜茜 , 张龙斌 , 叶秀云 . BbRho5对球孢白僵菌生长速率的作用研究 . | 生物技术通报 , 2022 , 38 (2) , 132-140 . |
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