The　development　in　nanobiotechnology　provides　an　in-depth　understanding　of　cell-surface　interactions　at　the　nanoscale　level.　Particularly,　several　surface　features　have　shown　the　ability　to　interrogate　the　bacterial　behavior　and　fate.　In　the　past　decade,　the　mechanical　and　physical　sterilization　has　attracted　considerable　attention,　as　paradigms　of　such　do　not　rely　on　chemical　substances　to　damage　or　kill　bacteria,　whereas　it　is　associated　with　natural　living　organisms　or　synthetic　materials.　Of　note,　such　antibacterial　scenario　does　not　cause　bacterial　resistance,　as　the　morphology　of　nanometer　can　directly　cause　bacterial　death　through　physical　and　mechanical　interactions.　In　this　review,　we　provide　an　overview　of　recently　developed　technologies　of　leveraging　topographical　nanofeatures　for　physical　sterilization.　We　mainly　discuss　the　development　of　various　morphologic　nanostructures,　and　colloidal　nanostructures　show　casing　the　capacity　of　＂mechanical　sterilization.＂　Mechanically　sterilized　nanostructures　can　penetrate　or　cut　through　bacterial　membranes.　In　addition,　surface　morphology,　such　as　mechanical　bactericidal　nanoparticles　and　nanoneedles,　can　cause　damage　to　the　membrane　of　microorganisms,　leading　to　cell　lysis　and　death.　Although　the　research　in　the　field　of　mechanical　sterilization　is　still　in　infancy,　the　effect　of　these　nanostructure　morphologies　on　sterilization　has　shown　remarkable　antibacterial　potential,　which　could　provide　a　new　toolkit　for　anti-infection　and　antifouling　applications.