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利用嗜硫色谱和POROSHQ20离子交换色谱从蕲蛇蛇毒中纯化得到一种新型P-Ⅲ型蛇毒金属蛋白酶(snake venom metalloproteases SVMP)AA-ACA-I.该酶经SDS-PAGE测得分子量为47.6kD,含有链内二硫键,加DTT处理后,分子量为50.8kD,中性糖含量为3.5%,具有出血毒性和抗凝血活性.在70℃和pH9.0条件下保温60min,该酶会自降解成分子量30kD左右的新蛋白,降解得到的新蛋白抗凝血活性高于原蛋白.
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中国生物化学与分子生物学报
ISSN: 1007-7626
CN: 11-3870/Q
Year: 2009
Issue: 09
Volume: 25
Page: 811-817
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 4
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