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学者姓名：潘剑茹
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Abstract ：
Reactive oxygen species (ROS) are major contributors to radiation therapy-induced side effects in cancer patients. A fusion antioxidant enzyme comprising glutathione S-transferase (GST), superoxide dismutase 1 (SOD1), and a transmembrane peptide has been shown to effectively mitigate ROS-induced damage. To enhance its targeting capability, the fusion protein was further modified by incorporating a matrix metalloproteinase-2/9 substrate peptide (X) and the transmembrane peptide R9, yielding the antioxidant enzyme GST-SOD1-X-R9 (GS1XR). This modification reduced its transmembrane ability in tumor cells, thereby selectively protecting normal cells from oxidative stress. However, the use of non-human GST poses potential immunogenicity risks. In this study, we employed seamless cloning technology to construct an expression vector containing the human GST gene to replace the non-human GST gene, and then expressed and purified novel fusion antioxidant enzymes GS1R and GS1XR. The protective effects of newly constructed GS1R and GS1XR against hydrogen peroxide (H2O2)-induced oxidative damage in L-02 cells were then evaluated using GS1 as a control. Enzymatic activity assays revealed that the specific activity of GST in GS1XR remained unchanged compared to the unmodified protein, while SOD activity was enhanced. Exposure to 200 μmol/L H2O2 transiently activated the nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway; however, this activation diminished after 24 h, reducing cell viability to 48.4%. Both GS1R and GS1XR effectively scavenged intracellular ROS, directly counteracting oxidative stress and promoting Nrf2 nuclear translocation, thereby activating antioxidant pathways and restoring cell viability to normal levels. The two enzymes showed comparable efficacy. In contrast, GS1, lacking transmembrane capability, was restricted to scavenging extracellular ROS and provided only limited protection. In conclusion, both novel fusion antioxidant enzymes demonstrated significant potential in safeguarding normal cells from ROS-mediated oxidative damage. The findings provide a foundation for further investigation in related field. ©2025 Chin J Biotech, All rights reserved.
Keyword ：
Biological radiation effects Biological radiation effects Clone cells Clone cells Peptides Peptides Radiation damage Radiation damage Transcription Transcription
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| GB/T 7714 | Pan, Jianru , Zhang, Ziyi , Chu, Jinnan et al. Construction of novel transmembrane fusion antioxidant enzymes and their protective effect against hydrogen peroxide-mediated cellular oxidative damage [J]. | Chinese Journal of Biotechnology , 2025 , 41 (4) : 1547-1558 . |
| MLA | Pan, Jianru et al. "Construction of novel transmembrane fusion antioxidant enzymes and their protective effect against hydrogen peroxide-mediated cellular oxidative damage" . | Chinese Journal of Biotechnology 41 . 4 (2025) : 1547-1558 . |
| APA | Pan, Jianru , Zhang, Ziyi , Chu, Jinnan , Han, Yanan , Zheng, Xueying , Cai, Shirong et al. Construction of novel transmembrane fusion antioxidant enzymes and their protective effect against hydrogen peroxide-mediated cellular oxidative damage . | Chinese Journal of Biotechnology , 2025 , 41 (4) , 1547-1558 . |
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目的:本研究旨在探讨融合抗氧化酶GST-SOD1-X-R9(GS1XR)对正常鼻咽上皮细胞NP69的放射防护作用及其可能的机制.方法:培养NP69细胞,分为空白对照(Untr)组、EGFP-GS1组、EGFP-GS1R组和EGFP-GS1XR组,检测0.5 mg/mL不同融合抗氧化酶的跨膜效应.用CCK-8法测定3种融合抗氧化酶在0～1 mg/mL质量浓度范围内的细胞毒性.以DCFH-DA荧光探针测定0～6 Gy X射线和不同剂量(0～1 mg/mL)GS1XR对NP69细胞内ROS水平的影响.进一步实验将NP69细胞分为Untr组、4 Gy X线单纯照射Ctrl组和照射前分别预处理GS1、GS1R、GS1XR及阿米福汀(AMFT,4μg/mL)组,检测细胞内ROS水平,流式细胞术检测细胞的凋亡率,用WB法检测Nrf2入核量、抗氧化基因GCLC以及抗凋亡因子Bcl-2和凋亡因子BAX的表达.结果:实验结果显示,EGFP-GS1不具备跨膜能力,而EGFP-GS1R和EGFP-GS1XR能够有效跨膜进入NP69细胞(P<0.0001).经24 h处理后,3种融合抗氧化酶均使细胞活力保持在80％以上,其中GS1XR处理组的细胞活力维持在100％以上.4 Gy X射线照射显著增加细胞内ROS水平(P<0.01),GS1XR以剂量依赖方式清除辐射诱导的ROS.与Ctrl组相比,GS1XR显著降低受照细胞内的ROS水平(P<0.05),促进Nrf2的入核(P<0.01),上调抗氧化基因GCLC(P<0.0001),降低细胞的凋亡率(P<0.0001)和抗凋亡因子Bcl-2(P<0.001)的表达,并下调促凋亡因子BAX的表达(P<0.05).GS1XR的整体保护作用与GS1R相似,且与阿米福汀效果相当.结论:融合抗氧化酶GS1XR对NP69细胞具有显著的放射防护效应,其机制可能与其可进入细胞清除受照细胞内ROS,激活Nrf2信号通路,并调节Bcl-2和BAX的表达有关,GS1XR有望成为一种新型的放射防护剂.
Keyword ：
放射防护 放射防护 融合抗氧化酶 融合抗氧化酶 鼻咽上皮细胞 鼻咽上皮细胞
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| GB/T 7714 | 何火聪 , 韩亚南 , 张紫怡 et al. 融合抗氧化酶GS1XR对鼻咽上皮细胞的放射防护效应 [J]. | 中国肿瘤生物治疗杂志 , 2024 , 31 (11) : 1116-1122 . |
| MLA | 何火聪 et al. "融合抗氧化酶GS1XR对鼻咽上皮细胞的放射防护效应" . | 中国肿瘤生物治疗杂志 31 . 11 (2024) : 1116-1122 . |
| APA | 何火聪 , 韩亚南 , 张紫怡 , 潘剑茹 . 融合抗氧化酶GS1XR对鼻咽上皮细胞的放射防护效应 . | 中国肿瘤生物治疗杂志 , 2024 , 31 (11) , 1116-1122 . |
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融合了跨膜肽的抗氧化酶可进入细胞，保护细胞免受放射损伤。然而跨膜肽的跨膜能力没有靶向性，其也可把抗氧化酶带入肿瘤细胞进而保护肿瘤细胞，降低放疗的效果。为此，根据多数肿瘤细胞微环境中存在活性基质金属蛋白酶(matrix metalloproteinase,MMP)-2或MMP-9的特点，在细胞跨膜肽R9与人铜、锌超氧化物歧化酶(superoxide dismutase 1,SOD1)和谷胱甘肽S-转移酶(glutathione S-transferase,GST)之间融合MMP-2/9的底物肽X，设计了融合蛋白GST-SOD1-X-R9。该蛋白在肿瘤微环境中可因MMP-2/9酶切底物肽X而失去跨...
Keyword ：
MMP-2/9敏感性 MMP-2/9敏感性 可跨膜 可跨膜 大肠杆菌表达体系 大肠杆菌表达体系 抗氧化酶 抗氧化酶 稳定性 稳定性 纯化 纯化
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| GB/T 7714 | 何火聪 , 林丽香 , 李玲玲 et al. 基质金属蛋白酶-2/9敏感型可跨膜融合抗氧化酶的表达、纯化和表征（英文） [J]. | 生物工程学报 , 2022 , 38 (09) : 3515-3527 . |
| MLA | 何火聪 et al. "基质金属蛋白酶-2/9敏感型可跨膜融合抗氧化酶的表达、纯化和表征（英文）" . | 生物工程学报 38 . 09 (2022) : 3515-3527 . |
| APA | 何火聪 , 林丽香 , 李玲玲 , 吴伦巧 , 林海英 , 潘剑茹 . 基质金属蛋白酶-2/9敏感型可跨膜融合抗氧化酶的表达、纯化和表征（英文） . | 生物工程学报 , 2022 , 38 (09) , 3515-3527 . |
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融合了跨膜肽的抗氧化酶可进入细胞,保护细胞免受放射损伤.然而跨膜肽的跨膜能力没有靶向性,其也可把抗氧化酶带入肿瘤细胞进而保护肿瘤细胞,降低放疗的效果.为此,根据多数肿瘤细胞微环境中存在活性基质金属蛋白酶(matrix metalloproteinase,MMP)-2或MMP-9的特点,在细胞跨膜肽R9与人铜、锌超氧化物歧化酶(superoxide dismutase 1,SOD1)和谷胱甘肽S-转移酶(glutathione S-transferase,GST)之间融合 MMP-2/9的底物肽X,设计了融合蛋白GST-SOD1-X-R9.该蛋白在肿瘤微环境中可因MMP-2/9酶切底物肽X而失去跨膜肽,从而无法进入肿瘤细胞,进而只能进入正常细胞.全基因合成SOD1-X-R9序列,并将其插入原核表达载体pGEX-4T-1中,得到表达质粒,并实现了GST-SOD1-X-R9融合蛋白的可溶表达.GST-SOD1-X-R9经硫酸铵沉淀和GST亲和层析纯化,分子量约为47kDa,与理论值一致.纯化的融合蛋白的SOD活性和GST活性分别为2 954 U/mg和328 U/mgoGST-SOD1-X-R9的SOD活性或GST活性在生理条件下几乎没有变化.该融合蛋白在溶液中可被胶原酶Ⅳ部分水解.分别建立了2D和3D培养的HepG2细胞模型来检验肿瘤微环境中的MMP-2活力对该蛋白跨膜能力的影响.在2D培养模型中,HepG2的MMP-2活力极低,但在3D培养模型中,随着培养时间的增加,HepG2肿瘤球的体积变大,其胞外MMP-2活力也随之增强.GST-SOD1-X-R9在2D培养的HepG2细胞中具有和GST-SOD1-R9蛋白一样的跨膜效率,但在3D培养的HepG2细胞球中的跨膜能力大大降低.本研究为后续深入研究GST-SOD1-X-R9靶向防护正常细胞的氧化损伤效应奠定了基础.
Keyword ：
MMP-2/9敏感性 MMP-2/9敏感性 可跨膜 可跨膜 大肠杆菌表达体系 大肠杆菌表达体系 抗氧化酶 抗氧化酶 稳定性 稳定性 纯化 纯化
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| GB/T 7714 | 何火聪 , 林丽香 , 李玲玲 et al. 基质金属蛋白酶-2/9敏感型可跨膜融合抗氧化酶的表达、纯化和表征 [J]. | 生物工程学报 , 2022 , 38 (9) : 3515-3527 . |
| MLA | 何火聪 et al. "基质金属蛋白酶-2/9敏感型可跨膜融合抗氧化酶的表达、纯化和表征" . | 生物工程学报 38 . 9 (2022) : 3515-3527 . |
| APA | 何火聪 , 林丽香 , 李玲玲 , 吴伦巧 , 林海英 , 潘剑茹 . 基质金属蛋白酶-2/9敏感型可跨膜融合抗氧化酶的表达、纯化和表征 . | 生物工程学报 , 2022 , 38 (9) , 3515-3527 . |
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Annexin A2 (ANXA2) has been found to be involved in cancer proliferation, metastasis and prognosis; however, its exact role in nasopharyngeal carcinoma (NPC) radioresistance remains unknown. We found that ANXA2 expression was correlated with prognosis in NPC patients, and longer overall survival in NPC patients with low ANXA2 expression than those with high ANXA2 expression. ANXA2 knockdown increased the radiosensitivity in radioresistant NPC cells, and ANXA2 overexpression decreased the radiosensitivity in NPC cells. Knocking-down ANXA2 expression increased the irradiation-induced apoptosis of radioresistant NPC cells, and ANXA2 overexpression decreased the irradiation-induced apoptosis of NPC cells. ANXA2 knockdown induced G2/M phase arrest in NPC cells post-irradiation, and ANXA2 overexpression abrogated G2/M phase arrest in NPC cells post-irradiation. ANXA2 overexpression resulted in inhibition of the p38 MAPK-HSP27 pathway, while ANXA2 knockdown resulted in activation of the p38 MAPK-HSP27 pathway. In addition, ANXA2 knockdown increased the radiosensitivity of the xenografted tumors in nude mice. Our data demonstrate that knockdown of Annexin A2 enhanced radiosensitivity in NPC by increasing G2/M-phase arrest, apoptosis and activating the p38 MAPK-HSP27 pathway. ANXA2 may be a promising target used to overcome radioresistance in NPC.
Keyword ：
annexin A2 annexin A2 apoptosis apoptosis cell-cycle arrest cell-cycle arrest nasopharyngeal carcinoma nasopharyngeal carcinoma radioresistance radioresistance
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| GB/T 7714 | He, Huocong , Lin, Keyu , Zou, Changyan et al. Knockdown of Annexin A2 Enhances Radiosensitivity by Increasing G2/M-Phase Arrest, Apoptosis and Activating the p38 MAPK-HSP27 Pathway in Nasopharyngeal Carcinoma [J]. | FRONTIERS IN ONCOLOGY , 2022 , 12 . |
| MLA | He, Huocong et al. "Knockdown of Annexin A2 Enhances Radiosensitivity by Increasing G2/M-Phase Arrest, Apoptosis and Activating the p38 MAPK-HSP27 Pathway in Nasopharyngeal Carcinoma" . | FRONTIERS IN ONCOLOGY 12 (2022) . |
| APA | He, Huocong , Lin, Keyu , Zou, Changyan , Pan, Jianru , Fu, Wankai , Zhou, Yan et al. Knockdown of Annexin A2 Enhances Radiosensitivity by Increasing G2/M-Phase Arrest, Apoptosis and Activating the p38 MAPK-HSP27 Pathway in Nasopharyngeal Carcinoma . | FRONTIERS IN ONCOLOGY , 2022 , 12 . |
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Background: Paraspeckle component 1 (PSPC1) is overexpressed in various cancer and correlated with poor survival in the patients. However, little is known about its expression and role in the progression of nasopharyngeal carcinomas (NPC). The purpose of this study is to examine PSPC1 expression in NPC and explore its role in clinical prognosis of radiation therapy. Methods: The association of PSPC1 expression with clinicopathological features of 109 NPC patients was examined using partial correlation analysis. Cancer tissues were obtained prior to clinical treatment. All cases were diagnosed and pathologically confirmed to be poorly differentiated or undifferentiated NPC without distant metastasis. The patients were then treated with radiation and followed-up. Survival analysis was performed. Results: Partial correlation analysis revealed that the PSPC1 expression in NPC was correlated with N classification, recurrence, prognosis and radiosensitivity in NPC patients, but not with the gender, age, pathohistological pattern, clinical stage, and T classification. The overexpression of PSPC1 was detected in 64 samples (58.72%). Kaplan-Meier survival analysis revealed that the overall survival (OS) was longer in NPC patients with PSPC1 low expression than that in those with PSPC1 high expression. Moreover, patients with the overexpression of PSPC1 had a low progression-free survival and distant metastasis-free survival rate, compared to those who had a low expression of PSPC1. Although not statistically significant, patients with high expression of PSPC1 had a lower locoregional recurrence-free survival rate than those with low expression, and the curves between the two groups was well separated. Conclusion: PSPC1 overexpression was associated with poor prognosis for NPC, which might be a novel useful biomarker to predict the response of NPC to radiation therapy and its clinical outcome.
Keyword ：
clinical prognosis clinical prognosis nasopharyngeal carcinoma nasopharyngeal carcinoma overexpression overexpression paraspeckle component 1 paraspeckle component 1 PSPC1 PSPC1 radiation radiation
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| GB/T 7714 | He, Huocong , Zhang, Lurong , Lin, Keyu et al. The Prognosis Value of PSPC1 Expression in Nasopharyngeal Cancer [J]. | CANCER MANAGEMENT AND RESEARCH , 2021 , 13 : 3281-3291 . |
| MLA | He, Huocong et al. "The Prognosis Value of PSPC1 Expression in Nasopharyngeal Cancer" . | CANCER MANAGEMENT AND RESEARCH 13 (2021) : 3281-3291 . |
| APA | He, Huocong , Zhang, Lurong , Lin, Keyu , Huang, Zhengrong , Zhou, Yan , Lin, Shaojun et al. The Prognosis Value of PSPC1 Expression in Nasopharyngeal Cancer . | CANCER MANAGEMENT AND RESEARCH , 2021 , 13 , 3281-3291 . |
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为了进一步研究当归(Angelica sinensis)生药中的蛋白质及其功能,通过80％硫酸铵沉淀、Sephadex G-50凝胶过滤层析、DEAE-Sepharose阴离子交换层析,首次从当归生药中纯化出两种分子量相近的蛋白(命名为ASPR-C-1和ASPR-C-2).ASPR-C-1和ASPR-C-2在SDS-PAGE上的分子量分别为17.33 kDa和17.18 kDa,在溶液中主要以单体形式存在,但会部分形成二聚体,二者均为糖蛋白,糖基含量分别为2.6％和8.2％.经基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-TOFTM)鉴定发现ASPR-C-1和ASPR-C-2均为病程相关(Pathogenesis-related 10,PR-10)家族蛋白,且具有核糖核酸酶活性,比活分别为73.60 U/mg和146.76 U/mg.两种蛋白的最适pH相近,均为5.6左右,但最适温度不同,ASPR-C-1的为50℃,ASPR-C-2的为60℃.二者虽然在60℃下都表现出最大的酶活力稳定性,但在更高的处理温度(80-100℃)下,ASPR-C-1迅速失活,最终仅余20％左右活力,ASPR-C-2则表现出良好的热稳定性,最终仍有80％左右活力.此外,Fe2+对二者的酶活性具有激活作用,而Ca2+、Mg2+、Zn2+、Mn2+、Ag+、Cu2+、EDTA、DTT和SDS则会不同程度地抑制二者的酶活性.研究结果为深入研究来自当归生药的PR-10蛋白的生物学功能奠定了基础.
Keyword ：
PR-10 PR-10 当归生药 当归生药 核糖核酸酶 核糖核酸酶 糖蛋白 糖蛋白 纯化 纯化
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| GB/T 7714 | 王香玲 , 李娴 , 何火聪 et al. 当归生药中两种PR-10蛋白亚型的纯化与表征 [J]. | 生物工程学报 , 2019 , 35 (1) : 159-168 . |
| MLA | 王香玲 et al. "当归生药中两种PR-10蛋白亚型的纯化与表征" . | 生物工程学报 35 . 1 (2019) : 159-168 . |
| APA | 王香玲 , 李娴 , 何火聪 , 李玲玲 , 吕迪 , 陈翠煌 et al. 当归生药中两种PR-10蛋白亚型的纯化与表征 . | 生物工程学报 , 2019 , 35 (1) , 159-168 . |
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In this study, two pathogenesis-related (PR) class 10 protein isoforms, ASPR-1 and ASPR-2, were purified from fresh roots of the Chinese medicinal plant Angelica sinensis (A. sinensis) using 80% ammonium sulfate precipitation, Sephadex G50 gel filtration chromatography, and DEAE-Sepharose ion-exchange chromatography. The molecular masses of ASPR-1 and ASPR-2 were estimated to be 16.66 kDa and 16.46 kDa, respectively, using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The isoforms are both glycoproteins containing glycosyl contents of 1.8% (ASPR-1) and 3.4% (ASPR-2). The two isoforms were predominantly present as monomers, but they partially dimerized in solution. The 15 N-terminal amino acids of ASPR-1 were determined to be GIQKTEVEAPSTVSA, with significant sequence homology to certain PR-10 proteins. ASPR-2 was also identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) analysis to be a PR-10 protein. The isoforms both exhibited ribonuclease (RNase) activity, with ASPR-2 having higher specific activity (128.85 U mg(-1)) than ASPR-1 (68.67 U mg(-1)). The isoforms had the same optimal temperature of 50 degrees C but different optimal pH values of 5.0 (ASPR-1) and 6.0 (ASPR-2). The RNase activities of the isoforms were both stable for 30 min at 50 degrees C, rapidly decreasing at higher or lower processing temperatures. However, ASPR-1 retained higher residual activity (89.4%-80.9%) than ASPR-2 (74.3%-67.9%) at temperatures from 40 degrees C to 60 degrees C. These results provide additional information to enrich the current knowledge of poorly annotated A. sinensis proteins.
Keyword ：
Angelica sinensis Angelica sinensis Glycoprotein Glycoprotein Isoform Isoform Pathogenesis-related (PR) class 10 protein Pathogenesis-related (PR) class 10 protein Purification Purification Ribonuclease activity Ribonuclease activity
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| GB/T 7714 | Pan, Jianru , Wang, Xiangling , Li, Lingling et al. Purification and characterization of two pathogenesis-related class 10 protein isoforms with ribonuclease activity from the fresh Angelica sinensis roots [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2018 , 128 : 66-71 . |
| MLA | Pan, Jianru et al. "Purification and characterization of two pathogenesis-related class 10 protein isoforms with ribonuclease activity from the fresh Angelica sinensis roots" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 128 (2018) : 66-71 . |
| APA | Pan, Jianru , Wang, Xiangling , Li, Lingling , Li, Xian , Ye, Xiaoqiang , Lv, Di et al. Purification and characterization of two pathogenesis-related class 10 protein isoforms with ribonuclease activity from the fresh Angelica sinensis roots . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2018 , 128 , 66-71 . |
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目的探讨葡萄糖调节蛋白GRP78蛋白表达与鼻咽癌临床特征及预后的关系。方法随机选取2012年2月至2012年7月福建省肿瘤医院初诊鼻咽癌患者的治疗前鼻咽肿瘤活检组织标本107例,中位年龄47岁,全部病例鼻咽部组织均经病理学确诊且无远处转移。免疫组织化学法检测GRP78蛋白在鼻咽癌肿瘤组织的表达水平。根据鼻咽癌患者的性别、年龄、病理类型、肿瘤分期、无远处转移生存时间和总生存时间等临床资料,分析GRP78蛋白表达与临床分期、肿瘤远处转移和总生存等的关系。结果全部鼻咽肿瘤活检组织标本中GRP78蛋白的阳性表达率为72.9%(78/107)。GRP78蛋白的表达水平与患者的性别、年龄、病理类型、临床分...
Keyword ：
GRP78 GRP78 临床分期 临床分期 临床意义 临床意义 低表达 低表达 活检组织 活检组织 高表达 高表达 鼻咽癌患者 鼻咽癌患者 鼻咽肿瘤 鼻咽肿瘤
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| GB/T 7714 | 何火聪 , 苏颖 , 林可焴 et al. GRP78在鼻咽癌组织的表达及其临床意义 [C] //2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛论文集 . 2018 . |
| MLA | 何火聪 et al. "GRP78在鼻咽癌组织的表达及其临床意义" 2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛论文集 . (2018) . |
| APA | 何火聪 , 苏颖 , 林可焴 , 陈超 , 邹长棪 , 潘剑茹 . GRP78在鼻咽癌组织的表达及其临床意义 2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛论文集 . (2018) . |
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Abstract ：
本发明涉及天然中药蛋白领域，更具体地涉及一种当归蛋白在制备防护急性肝损伤药物中的应用，所述当归蛋白N端序列为GIQKTEVEAPSTVSA。所述当归蛋白对正常肝细胞L‑02具有显著的促增殖作用，在0.5 mg/mL的剂量下，可使L‑02细胞的增殖率达到163.14%。所述当归蛋白用于制备预防肝损伤药物，可显著提高损伤鼠肝脏CAT、GST和T‑AOC活力极显著地减轻肝脏MDA水平，显著改善损伤鼠肝脏的肝细胞坏死状况，进而显著降低损伤鼠血液ALT、AST活力及肝脏指数，使之基本恢复至正常组水平，从而显著减轻CCl4对小鼠肝脏的损伤。
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| GB/T 7714 | 潘剑茹 , 王香玲 . 一种当归蛋白在制备防护肝损伤药物中的应用 : CN201810421947.8[P]. | 2018/5/4 . |
| MLA | 潘剑茹 et al. "一种当归蛋白在制备防护肝损伤药物中的应用" : CN201810421947.8. | 2018/5/4 . |
| APA | 潘剑茹 , 王香玲 . 一种当归蛋白在制备防护肝损伤药物中的应用 : CN201810421947.8. | 2018/5/4 . |
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